Experiment: KB with Oxedrine 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1901 and Psyr_1902 are separated by 42 nucleotides Psyr_1902 and Psyr_1903 are separated by 41 nucleotides Psyr_1903 and Psyr_1904 are separated by 102 nucleotides
Psyr_1901: Psyr_1901 - FAD-dependent pyridine nucleotide-disulfide oxidoreductase:Pyridine nucleotide-disulfide oxidoreductase dimerization region, at 2,176,378 to 2,177,811
_1901
Psyr_1902: Psyr_1902 - Glycerophosphoryl diester phosphodiesterase, at 2,177,854 to 2,178,576
_1902
Psyr_1903: Psyr_1903 - conserved hypothetical protein, at 2,178,618 to 2,179,205
_1903
Psyr_1904: Psyr_1904 - Protein of unknown function DUF214, at 2,179,308 to 2,180,558
_1904
Position (kb)
2178
2179
2180 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2178.356 kb on + strand, within Psyr_1902 at 2178.357 kb on - strand, within Psyr_1902 at 2178.357 kb on - strand, within Psyr_1902 at 2178.357 kb on - strand, within Psyr_1902 at 2178.563 kb on + strand at 2178.567 kb on + strand at 2178.568 kb on - strand at 2178.580 kb on + strand at 2178.580 kb on + strand at 2178.581 kb on - strand at 2178.597 kb on - strand at 2178.697 kb on - strand, within Psyr_1903 at 2178.742 kb on + strand, within Psyr_1903 at 2178.742 kb on + strand, within Psyr_1903 at 2178.742 kb on + strand, within Psyr_1903 at 2178.742 kb on + strand, within Psyr_1903 at 2178.743 kb on - strand, within Psyr_1903 at 2179.194 kb on + strand at 2179.194 kb on + strand at 2179.194 kb on + strand at 2179.194 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Oxedrine 10 mM remove 2,178,356 + Psyr_1902 0.69 +0.1 2,178,357 - Psyr_1902 0.70 +1.4 2,178,357 - Psyr_1902 0.70 +1.0 2,178,357 - Psyr_1902 0.70 +3.0 2,178,563 + +0.9 2,178,567 + +0.8 2,178,568 - +1.9 2,178,580 + +0.4 2,178,580 + +0.6 2,178,581 - -0.4 2,178,597 - -0.2 2,178,697 - Psyr_1903 0.13 +2.4 2,178,742 + Psyr_1903 0.21 -0.2 2,178,742 + Psyr_1903 0.21 +0.4 2,178,742 + Psyr_1903 0.21 -0.4 2,178,742 + Psyr_1903 0.21 -0.1 2,178,743 - Psyr_1903 0.21 -0.1 2,179,194 + -0.6 2,179,194 + -1.8 2,179,194 + +0.3 2,179,194 + -0.8
Or see this region's nucleotide sequence