Strain Fitness in Pseudomonas syringae pv. syringae B728a ΔmexB around Psyr_0451

Experiment: KB with Oxedrine 10 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsyr_0449 and Psyr_0450 are separated by 1 nucleotidesPsyr_0450 and Psyr_0451 are separated by 1 nucleotidesPsyr_0451 and Psyr_0452 are separated by 122 nucleotidesPsyr_0452 and Psyr_0453 are separated by 106 nucleotides Psyr_0449: Psyr_0449 - Malonate transporter MadL subunit, at 485,743 to 486,159 _0449 Psyr_0450: Psyr_0450 - Malonate/sodium symporter MadM subunit, at 486,161 to 486,925 _0450 Psyr_0451: Psyr_0451 - regulatory protein, LysR:LysR, substrate-binding protein, at 486,927 to 487,853 _0451 Psyr_0452: Psyr_0452 - HylII, at 487,976 to 488,590 _0452 Psyr_0453: Psyr_0453 - 16S rRNA m(3)U-1498 methyltransferase, at 488,697 to 489,416 _0453 Position (kb) 486 487 488Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 485.953 kb on + strand, within Psyr_0449at 485.953 kb on + strand, within Psyr_0449at 485.954 kb on - strand, within Psyr_0449at 485.954 kb on - strand, within Psyr_0449at 486.160 kb on + strandat 486.160 kb on + strandat 486.160 kb on + strandat 486.161 kb on - strandat 486.161 kb on - strandat 486.161 kb on - strandat 486.487 kb on - strand, within Psyr_0450at 486.602 kb on + strand, within Psyr_0450at 486.602 kb on + strand, within Psyr_0450at 486.602 kb on + strand, within Psyr_0450at 486.602 kb on + strand, within Psyr_0450at 486.602 kb on + strand, within Psyr_0450at 486.602 kb on + strand, within Psyr_0450at 486.602 kb on + strand, within Psyr_0450at 486.602 kb on + strand, within Psyr_0450at 486.602 kb on + strand, within Psyr_0450at 486.602 kb on + strand, within Psyr_0450at 486.603 kb on - strand, within Psyr_0450at 486.603 kb on - strand, within Psyr_0450at 486.603 kb on - strand, within Psyr_0450at 486.603 kb on - strand, within Psyr_0450at 486.603 kb on - strand, within Psyr_0450at 486.603 kb on - strand, within Psyr_0450at 486.603 kb on - strand, within Psyr_0450at 486.651 kb on + strand, within Psyr_0450at 486.651 kb on + strand, within Psyr_0450at 486.651 kb on + strand, within Psyr_0450at 486.651 kb on + strand, within Psyr_0450at 486.651 kb on + strand, within Psyr_0450at 486.652 kb on - strand, within Psyr_0450at 486.834 kb on - strand, within Psyr_0450at 486.896 kb on + strandat 486.896 kb on + strandat 486.897 kb on - strandat 486.897 kb on - strandat 486.897 kb on - strandat 486.923 kb on + strandat 486.923 kb on + strandat 486.958 kb on + strandat 486.958 kb on + strandat 486.959 kb on - strandat 486.959 kb on - strandat 486.959 kb on - strandat 486.959 kb on - strandat 487.087 kb on + strand, within Psyr_0451at 487.210 kb on - strand, within Psyr_0451at 487.435 kb on + strand, within Psyr_0451at 487.435 kb on + strand, within Psyr_0451at 487.435 kb on + strand, within Psyr_0451at 487.435 kb on + strand, within Psyr_0451at 487.435 kb on + strand, within Psyr_0451at 487.436 kb on - strand, within Psyr_0451at 487.436 kb on - strand, within Psyr_0451at 487.436 kb on - strand, within Psyr_0451at 487.436 kb on - strand, within Psyr_0451at 487.436 kb on - strand, within Psyr_0451at 487.472 kb on + strand, within Psyr_0451at 487.541 kb on - strand, within Psyr_0451at 487.651 kb on + strand, within Psyr_0451at 487.651 kb on + strand, within Psyr_0451at 487.651 kb on + strand, within Psyr_0451at 487.651 kb on + strand, within Psyr_0451at 487.652 kb on - strand, within Psyr_0451at 487.652 kb on - strand, within Psyr_0451at 487.813 kb on - strandat 487.894 kb on + strandat 487.904 kb on + strandat 487.904 kb on + strandat 487.904 kb on + strandat 487.904 kb on + strandat 487.904 kb on + strandat 487.905 kb on - strandat 487.905 kb on - strandat 487.905 kb on - strandat 487.905 kb on - strandat 487.905 kb on - strandat 487.905 kb on - strandat 487.905 kb on - strandat 487.905 kb on - strandat 487.905 kb on - strandat 487.905 kb on - strandat 487.905 kb on - strandat 487.947 kb on + strandat 487.979 kb on + strandat 487.979 kb on + strandat 487.979 kb on + strandat 487.979 kb on + strandat 487.979 kb on + strandat 487.980 kb on - strandat 487.980 kb on - strandat 487.980 kb on - strandat 487.980 kb on - strandat 487.980 kb on - strandat 487.983 kb on - strandat 488.108 kb on + strand, within Psyr_0452at 488.108 kb on + strand, within Psyr_0452at 488.108 kb on + strand, within Psyr_0452at 488.108 kb on + strand, within Psyr_0452at 488.108 kb on + strand, within Psyr_0452at 488.108 kb on + strand, within Psyr_0452at 488.108 kb on + strand, within Psyr_0452at 488.108 kb on + strand, within Psyr_0452at 488.108 kb on + strand, within Psyr_0452at 488.108 kb on + strand, within Psyr_0452at 488.108 kb on + strand, within Psyr_0452at 488.108 kb on + strand, within Psyr_0452at 488.108 kb on + strand, within Psyr_0452at 488.108 kb on + strand, within Psyr_0452at 488.108 kb on + strand, within Psyr_0452at 488.109 kb on - strand, within Psyr_0452at 488.109 kb on - strand, within Psyr_0452at 488.109 kb on - strand, within Psyr_0452at 488.109 kb on - strand, within Psyr_0452at 488.109 kb on - strand, within Psyr_0452at 488.109 kb on - strand, within Psyr_0452at 488.109 kb on - strand, within Psyr_0452at 488.109 kb on - strand, within Psyr_0452at 488.109 kb on - strand, within Psyr_0452at 488.109 kb on - strand, within Psyr_0452at 488.109 kb on - strand, within Psyr_0452at 488.109 kb on - strand, within Psyr_0452at 488.109 kb on - strand, within Psyr_0452at 488.132 kb on + strand, within Psyr_0452at 488.133 kb on - strand, within Psyr_0452at 488.150 kb on + strand, within Psyr_0452at 488.151 kb on - strand, within Psyr_0452at 488.210 kb on + strand, within Psyr_0452at 488.210 kb on + strand, within Psyr_0452at 488.211 kb on - strand, within Psyr_0452at 488.211 kb on - strand, within Psyr_0452at 488.232 kb on - strand, within Psyr_0452at 488.232 kb on - strand, within Psyr_0452at 488.328 kb on + strand, within Psyr_0452at 488.328 kb on + strand, within Psyr_0452at 488.328 kb on + strand, within Psyr_0452at 488.329 kb on - strand, within Psyr_0452at 488.469 kb on - strand, within Psyr_0452at 488.469 kb on - strand, within Psyr_0452at 488.469 kb on - strand, within Psyr_0452at 488.579 kb on + strandat 488.579 kb on + strandat 488.580 kb on - strandat 488.622 kb on + strandat 488.622 kb on + strandat 488.622 kb on + strandat 488.622 kb on + strandat 488.622 kb on + strandat 488.623 kb on - strandat 488.623 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction KB with Oxedrine 10 mM
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485,953 + Psyr_0449 0.50 +0.1
485,953 + Psyr_0449 0.50 -0.7
485,954 - Psyr_0449 0.51 +1.1
485,954 - Psyr_0449 0.51 +0.8
486,160 + -0.1
486,160 + -0.1
486,160 + +0.5
486,161 - -0.3
486,161 - +1.8
486,161 - -0.4
486,487 - Psyr_0450 0.43 +0.3
486,602 + Psyr_0450 0.58 -1.2
486,602 + Psyr_0450 0.58 -0.8
486,602 + Psyr_0450 0.58 +0.8
486,602 + Psyr_0450 0.58 +0.6
486,602 + Psyr_0450 0.58 -0.2
486,602 + Psyr_0450 0.58 -0.5
486,602 + Psyr_0450 0.58 -0.4
486,602 + Psyr_0450 0.58 +0.0
486,602 + Psyr_0450 0.58 -0.8
486,602 + Psyr_0450 0.58 +0.6
486,603 - Psyr_0450 0.58 -0.4
486,603 - Psyr_0450 0.58 -0.4
486,603 - Psyr_0450 0.58 -0.8
486,603 - Psyr_0450 0.58 +0.3
486,603 - Psyr_0450 0.58 -0.1
486,603 - Psyr_0450 0.58 +0.1
486,603 - Psyr_0450 0.58 -2.5
486,651 + Psyr_0450 0.64 +1.0
486,651 + Psyr_0450 0.64 +0.0
486,651 + Psyr_0450 0.64 +1.5
486,651 + Psyr_0450 0.64 +0.9
486,651 + Psyr_0450 0.64 +0.5
486,652 - Psyr_0450 0.64 +0.0
486,834 - Psyr_0450 0.88 -0.3
486,896 + +1.3
486,896 + +0.9
486,897 - -0.7
486,897 - -1.0
486,897 - +1.1
486,923 + -0.7
486,923 + -0.1
486,958 + -0.7
486,958 + -1.3
486,959 - +0.1
486,959 - -0.8
486,959 - +0.8
486,959 - -1.5
487,087 + Psyr_0451 0.17 +1.0
487,210 - Psyr_0451 0.31 -0.2
487,435 + Psyr_0451 0.55 +0.9
487,435 + Psyr_0451 0.55 +0.9
487,435 + Psyr_0451 0.55 +0.8
487,435 + Psyr_0451 0.55 -1.7
487,435 + Psyr_0451 0.55 +0.5
487,436 - Psyr_0451 0.55 +0.8
487,436 - Psyr_0451 0.55 +1.0
487,436 - Psyr_0451 0.55 -0.3
487,436 - Psyr_0451 0.55 +0.1
487,436 - Psyr_0451 0.55 -0.6
487,472 + Psyr_0451 0.59 -1.2
487,541 - Psyr_0451 0.66 -0.3
487,651 + Psyr_0451 0.78 -0.3
487,651 + Psyr_0451 0.78 +0.4
487,651 + Psyr_0451 0.78 +0.2
487,651 + Psyr_0451 0.78 +0.2
487,652 - Psyr_0451 0.78 +0.0
487,652 - Psyr_0451 0.78 +1.3
487,813 - -0.7
487,894 + +0.7
487,904 + +0.3
487,904 + +0.2
487,904 + -1.2
487,904 + +0.9
487,904 + -0.2
487,905 - -1.1
487,905 - -0.4
487,905 - +0.1
487,905 - +1.0
487,905 - +0.4
487,905 - +0.6
487,905 - -1.5
487,905 - +0.9
487,905 - -0.8
487,905 - +0.3
487,905 - -0.1
487,947 + -1.2
487,979 + +0.3
487,979 + -0.3
487,979 + +0.1
487,979 + +0.6
487,979 + +0.2
487,980 - -0.4
487,980 - -0.2
487,980 - -0.6
487,980 - -0.4
487,980 - +0.6
487,983 - -1.8
488,108 + Psyr_0452 0.21 -0.3
488,108 + Psyr_0452 0.21 -0.3
488,108 + Psyr_0452 0.21 +0.1
488,108 + Psyr_0452 0.21 -0.1
488,108 + Psyr_0452 0.21 +0.8
488,108 + Psyr_0452 0.21 +0.5
488,108 + Psyr_0452 0.21 +0.9
488,108 + Psyr_0452 0.21 +0.2
488,108 + Psyr_0452 0.21 -1.1
488,108 + Psyr_0452 0.21 -1.0
488,108 + Psyr_0452 0.21 -1.0
488,108 + Psyr_0452 0.21 -1.4
488,108 + Psyr_0452 0.21 +0.0
488,108 + Psyr_0452 0.21 -1.0
488,108 + Psyr_0452 0.21 -0.3
488,109 - Psyr_0452 0.22 +0.4
488,109 - Psyr_0452 0.22 -0.8
488,109 - Psyr_0452 0.22 -0.3
488,109 - Psyr_0452 0.22 +1.1
488,109 - Psyr_0452 0.22 +0.7
488,109 - Psyr_0452 0.22 -1.0
488,109 - Psyr_0452 0.22 +0.1
488,109 - Psyr_0452 0.22 -2.8
488,109 - Psyr_0452 0.22 +0.0
488,109 - Psyr_0452 0.22 +0.1
488,109 - Psyr_0452 0.22 +0.5
488,109 - Psyr_0452 0.22 -0.2
488,109 - Psyr_0452 0.22 +1.5
488,132 + Psyr_0452 0.25 +0.2
488,133 - Psyr_0452 0.26 -1.6
488,150 + Psyr_0452 0.28 +0.1
488,151 - Psyr_0452 0.28 -0.3
488,210 + Psyr_0452 0.38 +0.2
488,210 + Psyr_0452 0.38 -0.3
488,211 - Psyr_0452 0.38 -0.7
488,211 - Psyr_0452 0.38 +0.4
488,232 - Psyr_0452 0.42 +0.3
488,232 - Psyr_0452 0.42 -1.8
488,328 + Psyr_0452 0.57 -2.3
488,328 + Psyr_0452 0.57 +0.6
488,328 + Psyr_0452 0.57 +0.9
488,329 - Psyr_0452 0.57 -0.9
488,469 - Psyr_0452 0.80 -1.0
488,469 - Psyr_0452 0.80 +0.0
488,469 - Psyr_0452 0.80 +0.7
488,579 + +0.3
488,579 + -0.6
488,580 - +1.5
488,622 + -1.1
488,622 + +0.7
488,622 + +0.2
488,622 + +0.3
488,622 + -0.1
488,623 - +1.3
488,623 - -0.5

Or see this region's nucleotide sequence