Experiment: Acanthamoeba sp., muxed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF913 and GFF914 overlap by 4 nucleotides GFF914 and GFF915 are separated by 16 nucleotides GFF915 and GFF916 are separated by 77 nucleotides GFF916 and GFF917 overlap by 11 nucleotides GFF917 and GFF918 are separated by 3 nucleotides
GFF913 - D-Glucosaminate-6-phosphate ammonia-lyase (EC 4.3.1.-), at 90,937 to 92,055
GFF913
GFF914 - 2-dehydro-3-deoxyphosphogluconate aldolase (EC 4.1.2.14) in D-glucosaminate utilization operon, at 92,052 to 92,792
GFF914
GFF915 - Putative BglB-family transcriptional antiterminator, at 92,809 to 94,722
GFF915
GFF916 - RelB/StbD replicon stabilization protein (antitoxin to RelE/StbE), at 94,800 to 95,042
GFF916
GFF917 - RelE/StbE replicon stabilization toxin, at 95,032 to 95,316
GFF917
GFF918 - Ribonucleotide reductase of class III (anaerobic), activating protein (EC 1.97.1.4), at 95,320 to 95,784
GFF918
Position (kb) 92
93
94
95 Strain fitness (log2 ratio) -2
-1
0
1 at 91.811 kb on + strand, within GFF913 at 91.921 kb on + strand, within GFF913 at 91.930 kb on + strand, within GFF913 at 91.931 kb on - strand, within GFF913 at 92.001 kb on - strand at 92.220 kb on + strand, within GFF914 at 92.232 kb on - strand, within GFF914 at 92.311 kb on + strand, within GFF914 at 92.482 kb on + strand, within GFF914 at 92.568 kb on + strand, within GFF914 at 92.569 kb on - strand, within GFF914 at 92.570 kb on + strand, within GFF914 at 92.571 kb on - strand, within GFF914 at 92.578 kb on + strand, within GFF914 at 92.681 kb on - strand, within GFF914 at 92.703 kb on + strand, within GFF914 at 92.706 kb on - strand, within GFF914 at 92.706 kb on - strand, within GFF914 at 92.720 kb on + strand at 92.720 kb on + strand at 92.761 kb on + strand at 92.761 kb on + strand at 93.090 kb on - strand, within GFF915 at 93.094 kb on + strand, within GFF915 at 93.095 kb on - strand, within GFF915 at 93.308 kb on + strand, within GFF915 at 93.308 kb on + strand, within GFF915 at 93.309 kb on - strand, within GFF915 at 93.351 kb on + strand, within GFF915 at 93.352 kb on - strand, within GFF915 at 93.573 kb on - strand, within GFF915 at 93.575 kb on + strand, within GFF915 at 93.614 kb on + strand, within GFF915 at 93.634 kb on - strand, within GFF915 at 93.634 kb on - strand, within GFF915 at 93.922 kb on + strand, within GFF915 at 93.923 kb on - strand, within GFF915 at 93.925 kb on - strand, within GFF915 at 93.989 kb on - strand, within GFF915 at 94.019 kb on + strand, within GFF915 at 94.119 kb on - strand, within GFF915 at 94.229 kb on - strand, within GFF915 at 94.334 kb on + strand, within GFF915 at 94.441 kb on - strand, within GFF915 at 94.849 kb on - strand, within GFF916 at 94.898 kb on - strand, within GFF916 at 95.038 kb on + strand at 95.038 kb on + strand at 95.038 kb on + strand at 95.038 kb on + strand at 95.079 kb on + strand, within GFF917 at 95.080 kb on - strand, within GFF917 at 95.132 kb on + strand, within GFF917 at 95.132 kb on + strand, within GFF917 at 95.196 kb on + strand, within GFF917 at 95.315 kb on - strand at 95.485 kb on - strand, within GFF918 at 95.519 kb on - strand, within GFF918 at 95.519 kb on - strand, within GFF918 at 95.625 kb on + strand, within GFF918 at 95.626 kb on - strand, within GFF918 at 95.643 kb on + strand, within GFF918 at 95.684 kb on + strand, within GFF918
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2 remove 91,811 + GFF913 0.78 +0.2 91,921 + GFF913 0.88 +0.5 91,930 + GFF913 0.89 +0.3 91,931 - GFF913 0.89 +0.7 92,001 - -0.9 92,220 + GFF914 0.23 -0.2 92,232 - GFF914 0.24 +0.1 92,311 + GFF914 0.35 +0.7 92,482 + GFF914 0.58 -0.1 92,568 + GFF914 0.70 -1.0 92,569 - GFF914 0.70 +0.1 92,570 + GFF914 0.70 +0.4 92,571 - GFF914 0.70 -0.2 92,578 + GFF914 0.71 +0.3 92,681 - GFF914 0.85 -0.2 92,703 + GFF914 0.88 -0.8 92,706 - GFF914 0.88 -1.1 92,706 - GFF914 0.88 +0.3 92,720 + -0.2 92,720 + +0.6 92,761 + +0.0 92,761 + +0.3 93,090 - GFF915 0.15 -2.1 93,094 + GFF915 0.15 -0.3 93,095 - GFF915 0.15 +0.4 93,308 + GFF915 0.26 -0.6 93,308 + GFF915 0.26 -0.8 93,309 - GFF915 0.26 -0.7 93,351 + GFF915 0.28 -0.2 93,352 - GFF915 0.28 +0.3 93,573 - GFF915 0.40 -0.3 93,575 + GFF915 0.40 -0.7 93,614 + GFF915 0.42 -0.4 93,634 - GFF915 0.43 +0.4 93,634 - GFF915 0.43 -0.6 93,922 + GFF915 0.58 -0.0 93,923 - GFF915 0.58 +0.1 93,925 - GFF915 0.58 +0.0 93,989 - GFF915 0.62 -1.0 94,019 + GFF915 0.63 -0.7 94,119 - GFF915 0.68 -0.1 94,229 - GFF915 0.74 +1.1 94,334 + GFF915 0.80 +0.3 94,441 - GFF915 0.85 +0.2 94,849 - GFF916 0.20 +0.3 94,898 - GFF916 0.40 -0.2 95,038 + -0.4 95,038 + +0.4 95,038 + -0.1 95,038 + +0.2 95,079 + GFF917 0.16 -0.4 95,080 - GFF917 0.17 -0.6 95,132 + GFF917 0.35 -0.2 95,132 + GFF917 0.35 +1.1 95,196 + GFF917 0.58 +0.0 95,315 - -1.2 95,485 - GFF918 0.35 -0.1 95,519 - GFF918 0.43 -0.2 95,519 - GFF918 0.43 -0.2 95,625 + GFF918 0.66 +0.3 95,626 - GFF918 0.66 -0.6 95,643 + GFF918 0.69 +0.0 95,684 + GFF918 0.78 -0.0
Or see this region's nucleotide sequence