Experiment: Acanthamoeba sp., muxed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF904 and GFF905 are separated by 98 nucleotides GFF905 and GFF906 are separated by 3 nucleotides GFF906 and GFF907 are separated by 160 nucleotides GFF907 and GFF908 are separated by 10 nucleotides GFF908 and GFF909 overlap by 1 nucleotides
GFF904 - TldE protein, part of TldE/TldD proteolytic complex, at 85,190 to 86,530
GFF904
GFF905 - Soluble cytochrome b562, at 86,629 to 87,015
GFF905
GFF906 - FIG01047835: hypothetical protein, at 87,019 to 87,141
GFF906
GFF907 - FIG01045571: hypothetical protein, at 87,302 to 87,631
GFF907
GFF908 - FIG00628496: hypothetical protein, at 87,642 to 88,004
GFF908
GFF909 - FIG074102: hypothetical protein, at 88,004 to 88,306
GFF909
Position (kb)
87
88 Strain fitness (log2 ratio)
-1
0
1
2 at 86.171 kb on + strand, within GFF904 at 86.171 kb on + strand, within GFF904 at 86.339 kb on + strand, within GFF904 at 86.340 kb on - strand, within GFF904 at 86.405 kb on - strand at 86.440 kb on + strand at 86.479 kb on + strand at 86.481 kb on + strand at 86.481 kb on + strand at 86.599 kb on + strand at 86.645 kb on + strand at 86.709 kb on + strand, within GFF905 at 86.713 kb on - strand, within GFF905 at 86.991 kb on + strand at 86.991 kb on + strand at 86.992 kb on - strand at 87.000 kb on + strand at 87.001 kb on - strand at 87.001 kb on - strand at 87.001 kb on - strand at 87.007 kb on + strand at 87.070 kb on - strand, within GFF906 at 87.202 kb on - strand at 87.273 kb on - strand at 87.366 kb on + strand, within GFF907 at 87.469 kb on + strand, within GFF907 at 87.629 kb on + strand at 87.653 kb on + strand at 87.834 kb on - strand, within GFF908 at 87.859 kb on - strand, within GFF908 at 87.948 kb on - strand, within GFF908 at 88.038 kb on + strand, within GFF909 at 88.038 kb on + strand, within GFF909 at 88.123 kb on + strand, within GFF909 at 88.128 kb on + strand, within GFF909 at 88.128 kb on + strand, within GFF909 at 88.136 kb on - strand, within GFF909
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2 remove 86,171 + GFF904 0.73 -0.4 86,171 + GFF904 0.73 -0.4 86,339 + GFF904 0.86 -0.7 86,340 - GFF904 0.86 +0.1 86,405 - +0.8 86,440 + +2.0 86,479 + -0.1 86,481 + +0.0 86,481 + -0.5 86,599 + +0.2 86,645 + -0.0 86,709 + GFF905 0.21 +0.1 86,713 - GFF905 0.22 -0.2 86,991 + +0.2 86,991 + -1.5 86,992 - +0.3 87,000 + -0.0 87,001 - +0.0 87,001 - +0.1 87,001 - -0.0 87,007 + +0.7 87,070 - GFF906 0.41 -1.0 87,202 - +0.6 87,273 - +0.1 87,366 + GFF907 0.19 -0.5 87,469 + GFF907 0.51 -0.8 87,629 + -0.1 87,653 + -0.8 87,834 - GFF908 0.53 -0.3 87,859 - GFF908 0.60 +0.1 87,948 - GFF908 0.84 -0.4 88,038 + GFF909 0.11 -0.1 88,038 + GFF909 0.11 -0.1 88,123 + GFF909 0.39 -0.0 88,128 + GFF909 0.41 +0.6 88,128 + GFF909 0.41 +0.2 88,136 - GFF909 0.44 -0.0
Or see this region's nucleotide sequence