Experiment: Acanthamoeba sp., muxed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF78 and GFF79 overlap by 4 nucleotides GFF79 and GFF80 overlap by 1 nucleotides GFF80 and GFF81 are separated by 48 nucleotides
GFF78 - Ferric hydroxamate ABC transporter (TC 3.A.1.14.3), permease component FhuB, at 59,372 to 61,429
GFF78
GFF79 - Ferric hydroxamate ABC transporter (TC 3.A.1.14.3), periplasmic substrate binding protein FhuD, at 61,426 to 62,316
GFF79
GFF80 - Ferric hydroxamate ABC transporter (TC 3.A.1.14.3), ATP-binding protein FhuC, at 62,316 to 63,113
GFF80
GFF81 - Ferric hydroxamate outer membrane receptor FhuA, at 63,162 to 65,273
GFF81
Position (kb) 61
62
63 Strain fitness (log2 ratio) -1
0
1
2 at 60.473 kb on - strand, within GFF78 at 60.866 kb on + strand, within GFF78 at 61.110 kb on - strand, within GFF78 at 61.235 kb on - strand at 61.374 kb on - strand at 61.374 kb on - strand at 61.449 kb on + strand at 61.493 kb on + strand at 61.493 kb on + strand at 61.494 kb on - strand at 61.626 kb on + strand, within GFF79 at 61.627 kb on - strand, within GFF79 at 61.628 kb on + strand, within GFF79 at 61.629 kb on - strand, within GFF79 at 61.755 kb on + strand, within GFF79 at 61.756 kb on - strand, within GFF79 at 61.845 kb on - strand, within GFF79 at 61.845 kb on - strand, within GFF79 at 61.945 kb on - strand, within GFF79 at 62.123 kb on + strand, within GFF79 at 62.124 kb on - strand, within GFF79 at 62.281 kb on + strand at 62.295 kb on + strand at 62.304 kb on - strand at 62.317 kb on + strand at 62.318 kb on - strand at 62.318 kb on - strand at 62.320 kb on + strand at 62.320 kb on + strand at 62.381 kb on - strand at 62.464 kb on + strand, within GFF80 at 62.704 kb on - strand, within GFF80 at 62.730 kb on + strand, within GFF80 at 62.848 kb on + strand, within GFF80 at 62.966 kb on + strand, within GFF80 at 62.987 kb on + strand, within GFF80 at 62.988 kb on - strand, within GFF80 at 63.087 kb on - strand at 63.220 kb on + strand at 63.263 kb on + strand at 63.316 kb on + strand at 63.316 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2 remove 60,473 - GFF78 0.53 +0.2 60,866 + GFF78 0.73 +0.5 61,110 - GFF78 0.84 +0.2 61,235 - +0.3 61,374 - -0.7 61,374 - +0.3 61,449 + +0.6 61,493 + +0.7 61,493 + +0.3 61,494 - +2.2 61,626 + GFF79 0.22 +0.5 61,627 - GFF79 0.23 -0.2 61,628 + GFF79 0.23 +0.0 61,629 - GFF79 0.23 +0.0 61,755 + GFF79 0.37 +0.2 61,756 - GFF79 0.37 +1.5 61,845 - GFF79 0.47 +0.3 61,845 - GFF79 0.47 +0.7 61,945 - GFF79 0.58 +0.1 62,123 + GFF79 0.78 +0.1 62,124 - GFF79 0.78 +0.2 62,281 + +0.5 62,295 + +0.3 62,304 - -0.8 62,317 + +0.2 62,318 - -0.3 62,318 - +0.1 62,320 + -1.1 62,320 + +0.4 62,381 - +0.5 62,464 + GFF80 0.19 +0.4 62,704 - GFF80 0.49 -0.2 62,730 + GFF80 0.52 -0.3 62,848 + GFF80 0.67 +0.2 62,966 + GFF80 0.81 +0.3 62,987 + GFF80 0.84 +1.6 62,988 - GFF80 0.84 -1.4 63,087 - +0.4 63,220 + -0.2 63,263 + +0.9 63,316 + +0.0 63,316 + +0.0
Or see this region's nucleotide sequence