Experiment: Acanthamoeba sp., muxed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF722 and GFF723 are separated by 5 nucleotides GFF723 and GFF724 are separated by 277 nucleotides
GFF722 - Pyruvate formate-lyase (EC 2.3.1.54), at 78,411 to 80,843
GFF722
GFF723 - Pyruvate formate-lyase activating enzyme (EC 1.97.1.4), at 80,849 to 81,748
GFF723
GFF724 - Molybdopterin biosynthesis protein MoeB, at 82,026 to 82,775
GFF724
Position (kb) 80
81
82 Strain fitness (log2 ratio) -2
-1
0
1 at 79.866 kb on + strand, within GFF722 at 79.866 kb on + strand, within GFF722 at 80.038 kb on + strand, within GFF722 at 80.039 kb on - strand, within GFF722 at 80.039 kb on - strand, within GFF722 at 80.111 kb on + strand, within GFF722 at 80.112 kb on - strand, within GFF722 at 80.115 kb on + strand, within GFF722 at 80.115 kb on + strand, within GFF722 at 80.115 kb on + strand, within GFF722 at 80.115 kb on + strand, within GFF722 at 80.116 kb on - strand, within GFF722 at 80.116 kb on - strand, within GFF722 at 80.116 kb on - strand, within GFF722 at 80.124 kb on - strand, within GFF722 at 80.435 kb on - strand, within GFF722 at 80.490 kb on - strand, within GFF722 at 80.574 kb on - strand, within GFF722 at 80.651 kb on + strand at 80.652 kb on - strand at 80.725 kb on + strand at 80.737 kb on + strand at 80.778 kb on - strand at 80.847 kb on - strand at 80.847 kb on - strand at 80.890 kb on - strand at 80.909 kb on + strand at 80.946 kb on + strand, within GFF723 at 80.960 kb on + strand, within GFF723 at 80.971 kb on + strand, within GFF723 at 81.226 kb on + strand, within GFF723 at 81.227 kb on - strand, within GFF723 at 81.228 kb on + strand, within GFF723 at 81.228 kb on + strand, within GFF723 at 81.440 kb on + strand, within GFF723 at 81.615 kb on - strand, within GFF723 at 81.692 kb on + strand at 81.693 kb on - strand at 81.727 kb on - strand at 81.857 kb on - strand at 81.892 kb on - strand at 81.969 kb on - strand at 81.979 kb on - strand at 82.006 kb on + strand at 82.099 kb on + strand at 82.100 kb on - strand at 82.100 kb on - strand at 82.100 kb on - strand at 82.149 kb on - strand, within GFF724 at 82.283 kb on + strand, within GFF724 at 82.283 kb on + strand, within GFF724 at 82.330 kb on - strand, within GFF724 at 82.346 kb on + strand, within GFF724 at 82.349 kb on - strand, within GFF724 at 82.374 kb on + strand, within GFF724 at 82.374 kb on + strand, within GFF724 at 82.375 kb on - strand, within GFF724 at 82.434 kb on - strand, within GFF724 at 82.467 kb on - strand, within GFF724 at 82.475 kb on + strand, within GFF724 at 82.475 kb on + strand, within GFF724 at 82.515 kb on + strand, within GFF724 at 82.727 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2 remove 79,866 + GFF722 0.60 -0.3 79,866 + GFF722 0.60 -0.1 80,038 + GFF722 0.67 -0.2 80,039 - GFF722 0.67 +0.4 80,039 - GFF722 0.67 -0.0 80,111 + GFF722 0.70 +0.0 80,112 - GFF722 0.70 +0.1 80,115 + GFF722 0.70 +0.1 80,115 + GFF722 0.70 -0.0 80,115 + GFF722 0.70 +0.0 80,115 + GFF722 0.70 +0.3 80,116 - GFF722 0.70 -0.6 80,116 - GFF722 0.70 +1.6 80,116 - GFF722 0.70 +0.2 80,124 - GFF722 0.70 -0.2 80,435 - GFF722 0.83 +0.6 80,490 - GFF722 0.85 +0.2 80,574 - GFF722 0.89 +0.0 80,651 + -0.1 80,652 - -1.0 80,725 + -0.5 80,737 + -0.3 80,778 - +0.9 80,847 - +0.9 80,847 - -0.0 80,890 - -0.5 80,909 + +0.2 80,946 + GFF723 0.11 +1.0 80,960 + GFF723 0.12 +0.2 80,971 + GFF723 0.14 -0.6 81,226 + GFF723 0.42 +0.8 81,227 - GFF723 0.42 -0.0 81,228 + GFF723 0.42 +0.3 81,228 + GFF723 0.42 -0.3 81,440 + GFF723 0.66 +1.7 81,615 - GFF723 0.85 +0.5 81,692 + +0.6 81,693 - -0.1 81,727 - +0.3 81,857 - +0.2 81,892 - +0.9 81,969 - +0.1 81,979 - +0.7 82,006 + +0.1 82,099 + -0.1 82,100 - +0.0 82,100 - +0.1 82,100 - -0.7 82,149 - GFF724 0.16 -0.3 82,283 + GFF724 0.34 -0.7 82,283 + GFF724 0.34 -0.3 82,330 - GFF724 0.41 -0.3 82,346 + GFF724 0.43 -0.0 82,349 - GFF724 0.43 -1.7 82,374 + GFF724 0.46 -2.2 82,374 + GFF724 0.46 +1.0 82,375 - GFF724 0.47 -0.2 82,434 - GFF724 0.54 -0.3 82,467 - GFF724 0.59 -1.6 82,475 + GFF724 0.60 +1.7 82,475 + GFF724 0.60 -1.4 82,515 + GFF724 0.65 -0.7 82,727 + -1.9
Or see this region's nucleotide sequence