Experiment: Acanthamoeba sp., muxed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF678 and GFF679 are separated by 21 nucleotides GFF679 and GFF680 are separated by 2 nucleotides GFF680 and GFF681 overlap by 8 nucleotides GFF681 and GFF682 are separated by 1 nucleotides GFF682 and GFF683 are separated by 47 nucleotides
GFF678 - Molybdenum cofactor biosynthesis protein MoaA, at 37,702 to 38,691
GFF678
GFF679 - Molybdenum cofactor biosynthesis protein MoaB, at 38,713 to 39,225
GFF679
GFF680 - Molybdenum cofactor biosynthesis protein MoaC, at 39,228 to 39,713
GFF680
GFF681 - Molybdenum cofactor biosynthesis protein MoaD, at 39,706 to 39,951
GFF681
GFF682 - Molybdenum cofactor biosynthesis protein MoaE, at 39,953 to 40,405
GFF682
GFF683 - Putative membrane protein, at 40,453 to 41,157
GFF683
Position (kb)
39
40 Strain fitness (log2 ratio)
-1
0
1 at 38.322 kb on + strand, within GFF678 at 38.337 kb on - strand, within GFF678 at 38.519 kb on + strand, within GFF678 at 38.581 kb on + strand, within GFF678 at 38.581 kb on + strand, within GFF678 at 38.581 kb on + strand, within GFF678 at 38.582 kb on - strand, within GFF678 at 38.659 kb on - strand at 38.678 kb on - strand at 39.247 kb on + strand at 39.510 kb on - strand, within GFF680 at 39.635 kb on + strand, within GFF680 at 39.640 kb on + strand, within GFF680 at 39.641 kb on - strand, within GFF680 at 39.712 kb on - strand at 39.745 kb on - strand, within GFF681 at 40.403 kb on + strand at 40.403 kb on + strand at 40.510 kb on + strand at 40.510 kb on + strand at 40.510 kb on + strand at 40.511 kb on - strand at 40.526 kb on - strand, within GFF683 at 40.550 kb on + strand, within GFF683 at 40.585 kb on - strand, within GFF683 at 40.687 kb on - strand, within GFF683
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2 remove 38,322 + GFF678 0.63 -0.3 38,337 - GFF678 0.64 -0.5 38,519 + GFF678 0.83 -0.3 38,581 + GFF678 0.89 -0.4 38,581 + GFF678 0.89 -0.1 38,581 + GFF678 0.89 -0.3 38,582 - GFF678 0.89 +0.4 38,659 - -0.2 38,678 - -0.4 39,247 + +0.3 39,510 - GFF680 0.58 -0.1 39,635 + GFF680 0.84 -0.5 39,640 + GFF680 0.85 -1.0 39,641 - GFF680 0.85 -0.4 39,712 - -0.1 39,745 - GFF681 0.16 +0.2 40,403 + -0.4 40,403 + +0.0 40,510 + +0.2 40,510 + -0.6 40,510 + +0.2 40,511 - -0.2 40,526 - GFF683 0.10 -1.0 40,550 + GFF683 0.14 +0.0 40,585 - GFF683 0.19 -0.2 40,687 - GFF683 0.33 +1.0
Or see this region's nucleotide sequence