Experiment: Acanthamoeba sp., muxed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF671 and GFF672 overlap by 4 nucleotides GFF672 and GFF673 overlap by 17 nucleotides GFF673 and GFF674 overlap by 8 nucleotides
GFF671 - Biotin synthase (EC 2.8.1.6), at 27,234 to 28,274
GFF671
GFF672 - 8-amino-7-oxononanoate synthase (EC 2.3.1.47), at 28,271 to 29,428
GFF672
GFF673 - Biotin synthesis protein BioC, at 29,412 to 30,167
GFF673
GFF674 - Dethiobiotin synthetase (EC 6.3.3.3), at 30,160 to 30,846
GFF674
Position (kb) 28
29
30 Strain fitness (log2 ratio) -2
-1
0
1 at 27.353 kb on - strand, within GFF671 at 27.362 kb on + strand, within GFF671 at 27.363 kb on - strand, within GFF671 at 27.404 kb on + strand, within GFF671 at 27.405 kb on - strand, within GFF671 at 27.467 kb on - strand, within GFF671 at 27.476 kb on - strand, within GFF671 at 27.681 kb on + strand, within GFF671 at 27.681 kb on + strand, within GFF671 at 27.723 kb on - strand, within GFF671 at 27.738 kb on - strand, within GFF671 at 27.741 kb on + strand, within GFF671 at 27.741 kb on + strand, within GFF671 at 27.742 kb on - strand, within GFF671 at 27.788 kb on + strand, within GFF671 at 27.789 kb on - strand, within GFF671 at 27.789 kb on - strand, within GFF671 at 28.005 kb on + strand, within GFF671 at 28.005 kb on + strand, within GFF671 at 28.151 kb on + strand, within GFF671 at 28.254 kb on + strand at 28.255 kb on - strand at 28.324 kb on + strand at 28.341 kb on - strand at 28.341 kb on - strand at 28.391 kb on + strand, within GFF672 at 28.391 kb on + strand, within GFF672 at 28.392 kb on - strand, within GFF672 at 28.397 kb on - strand, within GFF672 at 28.425 kb on + strand, within GFF672 at 28.585 kb on + strand, within GFF672 at 28.802 kb on + strand, within GFF672 at 28.802 kb on + strand, within GFF672 at 28.802 kb on + strand, within GFF672 at 28.802 kb on + strand, within GFF672 at 28.803 kb on - strand, within GFF672 at 28.803 kb on - strand, within GFF672 at 28.803 kb on - strand, within GFF672 at 29.426 kb on + strand at 29.426 kb on + strand at 29.577 kb on - strand, within GFF673 at 29.577 kb on - strand, within GFF673 at 29.577 kb on - strand, within GFF673 at 29.790 kb on + strand, within GFF673 at 29.791 kb on - strand, within GFF673 at 30.080 kb on + strand, within GFF673 at 30.197 kb on - strand at 30.249 kb on - strand, within GFF674 at 30.249 kb on - strand, within GFF674 at 30.264 kb on - strand, within GFF674 at 30.264 kb on - strand, within GFF674 at 30.268 kb on + strand, within GFF674 at 30.268 kb on + strand, within GFF674 at 30.271 kb on - strand, within GFF674 at 30.384 kb on + strand, within GFF674 at 30.399 kb on + strand, within GFF674 at 30.399 kb on + strand, within GFF674
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2 remove 27,353 - GFF671 0.11 +1.0 27,362 + GFF671 0.12 +0.4 27,363 - GFF671 0.12 -0.0 27,404 + GFF671 0.16 +0.6 27,405 - GFF671 0.16 +0.2 27,467 - GFF671 0.22 +1.6 27,476 - GFF671 0.23 +0.5 27,681 + GFF671 0.43 -0.6 27,681 + GFF671 0.43 +0.3 27,723 - GFF671 0.47 -0.3 27,738 - GFF671 0.48 +0.2 27,741 + GFF671 0.49 +0.4 27,741 + GFF671 0.49 +0.5 27,742 - GFF671 0.49 -0.2 27,788 + GFF671 0.53 +0.4 27,789 - GFF671 0.53 -1.0 27,789 - GFF671 0.53 -0.3 28,005 + GFF671 0.74 -0.0 28,005 + GFF671 0.74 +0.1 28,151 + GFF671 0.88 -0.5 28,254 + +0.2 28,255 - +1.1 28,324 + -0.7 28,341 - +0.2 28,341 - -0.4 28,391 + GFF672 0.10 -0.2 28,391 + GFF672 0.10 +1.4 28,392 - GFF672 0.10 +0.4 28,397 - GFF672 0.11 +0.4 28,425 + GFF672 0.13 +0.3 28,585 + GFF672 0.27 -2.2 28,802 + GFF672 0.46 -0.7 28,802 + GFF672 0.46 -0.0 28,802 + GFF672 0.46 +1.2 28,802 + GFF672 0.46 -0.4 28,803 - GFF672 0.46 -0.6 28,803 - GFF672 0.46 -0.1 28,803 - GFF672 0.46 -0.1 29,426 + -1.2 29,426 + +0.8 29,577 - GFF673 0.22 +0.4 29,577 - GFF673 0.22 +0.3 29,577 - GFF673 0.22 -0.1 29,790 + GFF673 0.50 -1.8 29,791 - GFF673 0.50 +0.4 30,080 + GFF673 0.88 -0.0 30,197 - -0.3 30,249 - GFF674 0.13 +0.3 30,249 - GFF674 0.13 +0.5 30,264 - GFF674 0.15 -0.3 30,264 - GFF674 0.15 +0.3 30,268 + GFF674 0.16 +0.4 30,268 + GFF674 0.16 +0.6 30,271 - GFF674 0.16 +0.5 30,384 + GFF674 0.33 +0.2 30,399 + GFF674 0.35 +0.5 30,399 + GFF674 0.35 +0.1
Or see this region's nucleotide sequence