Experiment: Acanthamoeba sp., muxed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF59 and GFF60 are separated by 152 nucleotides GFF60 and GFF61 are separated by 129 nucleotides
GFF59 - Sugar diacid utilization regulator SdaR, at 41,860 to 43,017
GFF59
GFF60 - HtrA protease/chaperone protein, at 43,170 to 44,597
GFF60
GFF61 - Deoxyguanosinetriphosphate triphosphohydrolase (EC 3.1.5.1), subgroup 1, at 44,727 to 46,244
GFF61
Position (kb)
43
44
45 Strain fitness (log2 ratio)
-1
0
1
2 at 42.240 kb on + strand, within GFF59 at 42.260 kb on + strand, within GFF59 at 42.690 kb on + strand, within GFF59 at 42.690 kb on + strand, within GFF59 at 42.712 kb on - strand, within GFF59 at 42.712 kb on - strand, within GFF59 at 42.772 kb on + strand, within GFF59 at 42.888 kb on + strand, within GFF59 at 42.935 kb on - strand at 42.964 kb on - strand at 42.979 kb on - strand at 42.979 kb on - strand at 43.047 kb on + strand at 43.098 kb on + strand at 43.098 kb on + strand at 43.098 kb on + strand at 43.099 kb on - strand at 43.102 kb on + strand at 43.171 kb on + strand at 43.190 kb on + strand at 43.197 kb on - strand at 43.197 kb on - strand at 43.526 kb on + strand, within GFF60 at 43.785 kb on - strand, within GFF60 at 43.942 kb on - strand, within GFF60 at 44.027 kb on + strand, within GFF60 at 44.028 kb on - strand, within GFF60 at 44.028 kb on - strand, within GFF60 at 44.029 kb on + strand, within GFF60 at 44.029 kb on + strand, within GFF60 at 44.030 kb on - strand, within GFF60 at 44.172 kb on + strand, within GFF60 at 44.402 kb on + strand, within GFF60 at 44.403 kb on - strand, within GFF60 at 44.418 kb on - strand, within GFF60 at 44.418 kb on - strand, within GFF60 at 44.430 kb on - strand, within GFF60 at 44.581 kb on - strand at 44.640 kb on - strand at 44.992 kb on - strand, within GFF61 at 45.005 kb on - strand, within GFF61 at 45.012 kb on + strand, within GFF61 at 45.012 kb on + strand, within GFF61 at 45.188 kb on + strand, within GFF61 at 45.227 kb on + strand, within GFF61 at 45.279 kb on - strand, within GFF61 at 45.504 kb on - strand, within GFF61 at 45.504 kb on - strand, within GFF61 at 45.519 kb on - strand, within GFF61 at 45.538 kb on + strand, within GFF61
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2 remove 42,240 + GFF59 0.33 +0.6 42,260 + GFF59 0.35 -0.5 42,690 + GFF59 0.72 -0.4 42,690 + GFF59 0.72 +0.6 42,712 - GFF59 0.74 +0.4 42,712 - GFF59 0.74 -0.2 42,772 + GFF59 0.79 -0.0 42,888 + GFF59 0.89 -0.3 42,935 - -0.2 42,964 - +0.1 42,979 - -0.3 42,979 - +0.0 43,047 + +0.8 43,098 + -0.0 43,098 + -0.2 43,098 + +0.4 43,099 - -0.4 43,102 + -0.2 43,171 + -0.0 43,190 + -0.9 43,197 - +0.6 43,197 - -0.5 43,526 + GFF60 0.25 +0.5 43,785 - GFF60 0.43 +0.9 43,942 - GFF60 0.54 -0.3 44,027 + GFF60 0.60 +0.8 44,028 - GFF60 0.60 +0.8 44,028 - GFF60 0.60 +1.8 44,029 + GFF60 0.60 +0.9 44,029 + GFF60 0.60 -0.3 44,030 - GFF60 0.60 -0.1 44,172 + GFF60 0.70 -0.2 44,402 + GFF60 0.86 -0.4 44,403 - GFF60 0.86 +0.2 44,418 - GFF60 0.87 +0.7 44,418 - GFF60 0.87 -0.1 44,430 - GFF60 0.88 +0.8 44,581 - -0.1 44,640 - -0.1 44,992 - GFF61 0.17 -0.1 45,005 - GFF61 0.18 +0.3 45,012 + GFF61 0.19 -0.5 45,012 + GFF61 0.19 +0.3 45,188 + GFF61 0.30 +0.0 45,227 + GFF61 0.33 -0.1 45,279 - GFF61 0.36 +0.2 45,504 - GFF61 0.51 +0.7 45,504 - GFF61 0.51 +0.1 45,519 - GFF61 0.52 +0.3 45,538 + GFF61 0.53 +0.3
Or see this region's nucleotide sequence