Experiment: Acanthamoeba sp., muxed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4696 and GFF4697 overlap by 4 nucleotides GFF4697 and GFF4698 are separated by 11 nucleotides GFF4698 and GFF4699 are separated by 11 nucleotides GFF4699 and GFF4700 are separated by 75 nucleotides
GFF4696 - PTS system, glucitol/sorbitol-specific IIC component (EC 2.7.1.69), at 8,088 to 8,651
GFF4696
GFF4697 - PTS system, glucitol/sorbitol-specific IIB component and second of two IIC components (EC 2.7.1.69), at 8,648 to 9,619
GFF4697
GFF4698 - PTS system, glucitol/sorbitol-specific IIA component (EC 2.7.1.69), at 9,631 to 9,993
GFF4698
GFF4699 - Sorbitol-6-phosphate 2-dehydrogenase (EC 1.1.1.140), at 10,005 to 10,784
GFF4699
GFF4700 - Glucitol operon activator protein, at 10,860 to 11,219
GFF4700
Position (kb)
9
10 Strain fitness (log2 ratio)
-1
0
1
2 at 8.711 kb on - strand at 8.732 kb on + strand at 8.732 kb on + strand at 8.734 kb on + strand at 8.734 kb on + strand at 8.735 kb on - strand at 8.735 kb on - strand at 8.781 kb on + strand, within GFF4697 at 8.951 kb on + strand, within GFF4697 at 8.954 kb on - strand, within GFF4697 at 8.970 kb on - strand, within GFF4697 at 8.998 kb on + strand, within GFF4697 at 9.142 kb on - strand, within GFF4697 at 9.233 kb on - strand, within GFF4697 at 9.233 kb on - strand, within GFF4697 at 9.579 kb on + strand at 9.580 kb on - strand at 9.608 kb on - strand at 9.618 kb on - strand at 9.709 kb on - strand, within GFF4698 at 9.709 kb on - strand, within GFF4698 at 9.880 kb on - strand, within GFF4698 at 9.929 kb on + strand, within GFF4698 at 10.104 kb on - strand, within GFF4699 at 10.264 kb on - strand, within GFF4699 at 10.428 kb on - strand, within GFF4699 at 10.540 kb on + strand, within GFF4699 at 10.645 kb on - strand, within GFF4699 at 10.647 kb on + strand, within GFF4699 at 10.647 kb on + strand, within GFF4699 at 10.648 kb on - strand, within GFF4699 at 10.648 kb on - strand, within GFF4699 at 10.714 kb on - strand at 10.859 kb on + strand at 10.860 kb on - strand at 10.961 kb on + strand, within GFF4700
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2 remove 8,711 - +1.8 8,732 + +0.1 8,732 + +0.2 8,734 + -0.5 8,734 + -0.7 8,735 - +0.3 8,735 - +0.8 8,781 + GFF4697 0.14 -0.5 8,951 + GFF4697 0.31 +0.3 8,954 - GFF4697 0.31 -0.5 8,970 - GFF4697 0.33 -0.2 8,998 + GFF4697 0.36 +1.0 9,142 - GFF4697 0.51 -0.8 9,233 - GFF4697 0.60 -0.4 9,233 - GFF4697 0.60 +0.1 9,579 + -0.4 9,580 - +0.2 9,608 - +0.3 9,618 - -0.1 9,709 - GFF4698 0.21 +0.4 9,709 - GFF4698 0.21 +0.4 9,880 - GFF4698 0.69 +0.8 9,929 + GFF4698 0.82 +0.3 10,104 - GFF4699 0.13 -0.7 10,264 - GFF4699 0.33 -0.9 10,428 - GFF4699 0.54 -0.5 10,540 + GFF4699 0.69 +0.4 10,645 - GFF4699 0.82 +0.9 10,647 + GFF4699 0.82 -0.3 10,647 + GFF4699 0.82 +0.5 10,648 - GFF4699 0.82 -0.2 10,648 - GFF4699 0.82 -0.7 10,714 - +1.1 10,859 + +0.9 10,860 - -0.5 10,961 + GFF4700 0.28 +1.5
Or see this region's nucleotide sequence