Experiment: Acanthamoeba sp., muxed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF456 and GFF457 are separated by 149 nucleotides GFF457 and GFF458 are separated by 49 nucleotides GFF458 and GFF459 are separated by 218 nucleotides GFF459 and GFF460 are separated by 51 nucleotides
GFF456 - hypothetical protein, at 177,376 to 177,507
GFF456
GFF457 - probable exported protein YPO4070, at 177,657 to 178,367
GFF457
GFF458 - 2-ketoaldonate reductase, broad specificity (EC 1.1.1.215) (EC 1.1.1.-), at 178,417 to 179,391
GFF458
GFF459 - Outer membrane protein A precursor, at 179,610 to 180,272
GFF459
GFF460 - hypothetical protein, at 180,324 to 180,440
GFF460
Position (kb) 178
179
180 Strain fitness (log2 ratio) -1
0
1 at 177.434 kb on + strand, within GFF456 at 177.529 kb on + strand at 177.653 kb on - strand at 177.710 kb on - strand at 177.710 kb on - strand at 177.710 kb on - strand at 177.719 kb on + strand at 177.864 kb on + strand, within GFF457 at 177.876 kb on + strand, within GFF457 at 177.876 kb on + strand, within GFF457 at 177.876 kb on + strand, within GFF457 at 177.876 kb on + strand, within GFF457 at 177.877 kb on - strand, within GFF457 at 177.877 kb on - strand, within GFF457 at 177.967 kb on - strand, within GFF457 at 178.206 kb on + strand, within GFF457 at 178.206 kb on + strand, within GFF457 at 178.284 kb on + strand, within GFF457 at 178.284 kb on + strand, within GFF457 at 178.329 kb on - strand at 178.352 kb on - strand at 178.483 kb on - strand at 178.509 kb on - strand at 178.558 kb on - strand, within GFF458 at 178.628 kb on - strand, within GFF458 at 178.628 kb on - strand, within GFF458 at 178.695 kb on + strand, within GFF458 at 178.714 kb on - strand, within GFF458 at 178.806 kb on + strand, within GFF458 at 178.806 kb on + strand, within GFF458 at 178.821 kb on - strand, within GFF458 at 178.886 kb on + strand, within GFF458 at 178.888 kb on - strand, within GFF458 at 179.136 kb on + strand, within GFF458 at 179.157 kb on + strand, within GFF458 at 179.446 kb on + strand at 179.504 kb on - strand at 179.511 kb on + strand at 179.566 kb on - strand at 179.606 kb on + strand at 179.611 kb on + strand at 179.611 kb on + strand at 179.657 kb on + strand at 179.657 kb on + strand at 179.936 kb on + strand, within GFF459 at 179.957 kb on + strand, within GFF459 at 179.957 kb on + strand, within GFF459 at 179.958 kb on - strand, within GFF459 at 179.958 kb on - strand, within GFF459 at 180.034 kb on + strand, within GFF459 at 180.034 kb on + strand, within GFF459 at 180.034 kb on + strand, within GFF459 at 180.034 kb on + strand, within GFF459 at 180.035 kb on - strand, within GFF459 at 180.035 kb on - strand, within GFF459 at 180.092 kb on + strand, within GFF459 at 180.174 kb on - strand, within GFF459 at 180.219 kb on + strand at 180.220 kb on - strand at 180.259 kb on - strand at 180.259 kb on - strand at 180.284 kb on - strand at 180.378 kb on - strand, within GFF460
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2 remove 177,434 + GFF456 0.44 +1.5 177,529 + +0.3 177,653 - -0.6 177,710 - +0.2 177,710 - +0.2 177,710 - +0.2 177,719 + +0.6 177,864 + GFF457 0.29 -1.3 177,876 + GFF457 0.31 -0.2 177,876 + GFF457 0.31 -0.1 177,876 + GFF457 0.31 +0.1 177,876 + GFF457 0.31 -0.2 177,877 - GFF457 0.31 -0.0 177,877 - GFF457 0.31 +0.2 177,967 - GFF457 0.44 -0.0 178,206 + GFF457 0.77 -0.7 178,206 + GFF457 0.77 +0.2 178,284 + GFF457 0.88 -0.2 178,284 + GFF457 0.88 -0.5 178,329 - +0.3 178,352 - -0.1 178,483 - -0.2 178,509 - -0.2 178,558 - GFF458 0.14 +0.3 178,628 - GFF458 0.22 +0.0 178,628 - GFF458 0.22 -0.3 178,695 + GFF458 0.29 -1.1 178,714 - GFF458 0.30 +0.7 178,806 + GFF458 0.40 -0.7 178,806 + GFF458 0.40 +0.8 178,821 - GFF458 0.41 +0.3 178,886 + GFF458 0.48 -0.4 178,888 - GFF458 0.48 -0.1 179,136 + GFF458 0.74 +0.0 179,157 + GFF458 0.76 -0.3 179,446 + +0.4 179,504 - +0.0 179,511 + +0.2 179,566 - +1.7 179,606 + +0.1 179,611 + -0.3 179,611 + +0.4 179,657 + -0.2 179,657 + +0.1 179,936 + GFF459 0.49 -0.1 179,957 + GFF459 0.52 -0.4 179,957 + GFF459 0.52 +0.1 179,958 - GFF459 0.52 -1.2 179,958 - GFF459 0.52 +0.0 180,034 + GFF459 0.64 +1.1 180,034 + GFF459 0.64 +0.0 180,034 + GFF459 0.64 +0.2 180,034 + GFF459 0.64 +0.2 180,035 - GFF459 0.64 +0.4 180,035 - GFF459 0.64 +0.3 180,092 + GFF459 0.73 -0.0 180,174 - GFF459 0.85 -0.7 180,219 + +0.4 180,220 - -0.1 180,259 - -0.2 180,259 - +0.3 180,284 - -0.3 180,378 - GFF460 0.46 +0.2
Or see this region's nucleotide sequence