Experiment: Acanthamoeba sp., muxed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4501 and GFF4502 are separated by 13 nucleotides GFF4502 and GFF4503 overlap by 1 nucleotides GFF4503 and GFF4504 are separated by 2 nucleotides
GFF4501 - 2,3-dihydroxybenzoate-AMP ligase (EC 2.7.7.58) [enterobactin] siderophore, at 287,654 to 289,264
GFF4501
GFF4502 - Isochorismatase (EC 3.3.2.1) of siderophore biosynthesis, at 289,278 to 290,135
GFF4502
GFF4503 - 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase (EC 1.3.1.28) of siderophore biosynthesis, at 290,135 to 290,890
GFF4503
GFF4504 - Proofreading thioesterase in enterobactin biosynthesis EntH, at 290,893 to 291,306
GFF4504
Position (kb)
289
290
291 Strain fitness (log2 ratio)
-2
-1
0
1 at 288.278 kb on + strand, within GFF4501 at 288.356 kb on - strand, within GFF4501 at 288.359 kb on + strand, within GFF4501 at 288.360 kb on - strand, within GFF4501 at 288.360 kb on - strand, within GFF4501 at 288.514 kb on - strand, within GFF4501 at 288.994 kb on - strand, within GFF4501 at 289.083 kb on - strand, within GFF4501 at 289.093 kb on - strand, within GFF4501 at 289.093 kb on - strand, within GFF4501 at 289.120 kb on - strand at 289.302 kb on + strand at 289.388 kb on + strand, within GFF4502 at 289.389 kb on - strand, within GFF4502 at 289.478 kb on + strand, within GFF4502 at 289.479 kb on - strand, within GFF4502 at 289.503 kb on + strand, within GFF4502 at 289.556 kb on + strand, within GFF4502 at 289.556 kb on + strand, within GFF4502 at 289.556 kb on + strand, within GFF4502 at 289.557 kb on - strand, within GFF4502 at 289.604 kb on - strand, within GFF4502 at 289.654 kb on - strand, within GFF4502 at 289.739 kb on + strand, within GFF4502 at 289.852 kb on - strand, within GFF4502 at 289.852 kb on - strand, within GFF4502 at 290.015 kb on - strand, within GFF4502 at 290.051 kb on - strand at 290.133 kb on + strand at 290.386 kb on + strand, within GFF4503 at 290.548 kb on + strand, within GFF4503 at 290.563 kb on + strand, within GFF4503 at 290.584 kb on + strand, within GFF4503 at 290.651 kb on - strand, within GFF4503 at 290.842 kb on + strand at 290.842 kb on + strand at 290.843 kb on - strand at 290.974 kb on + strand, within GFF4504 at 290.975 kb on - strand, within GFF4504 at 291.103 kb on + strand, within GFF4504
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2 remove 288,278 + GFF4501 0.39 +0.4 288,356 - GFF4501 0.44 -0.0 288,359 + GFF4501 0.44 +0.3 288,360 - GFF4501 0.44 +0.1 288,360 - GFF4501 0.44 +0.3 288,514 - GFF4501 0.53 +0.2 288,994 - GFF4501 0.83 +0.2 289,083 - GFF4501 0.89 -0.1 289,093 - GFF4501 0.89 -0.6 289,093 - GFF4501 0.89 -0.2 289,120 - -0.3 289,302 + +0.5 289,388 + GFF4502 0.13 +0.0 289,389 - GFF4502 0.13 -0.4 289,478 + GFF4502 0.23 -0.5 289,479 - GFF4502 0.23 -0.7 289,503 + GFF4502 0.26 -0.3 289,556 + GFF4502 0.32 -0.5 289,556 + GFF4502 0.32 -0.9 289,556 + GFF4502 0.32 -0.4 289,557 - GFF4502 0.33 +0.5 289,604 - GFF4502 0.38 -2.0 289,654 - GFF4502 0.44 +0.4 289,739 + GFF4502 0.54 -0.0 289,852 - GFF4502 0.67 -0.2 289,852 - GFF4502 0.67 +0.9 290,015 - GFF4502 0.86 +0.7 290,051 - -2.3 290,133 + -0.1 290,386 + GFF4503 0.33 +0.1 290,548 + GFF4503 0.55 +0.4 290,563 + GFF4503 0.57 -0.1 290,584 + GFF4503 0.59 +0.2 290,651 - GFF4503 0.68 +0.3 290,842 + -0.1 290,842 + +0.1 290,843 - +0.6 290,974 + GFF4504 0.20 -0.0 290,975 - GFF4504 0.20 +0.2 291,103 + GFF4504 0.51 +0.3
Or see this region's nucleotide sequence