Experiment: Acanthamoeba sp., muxed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4416 and GFF4417 are separated by 36 nucleotides GFF4417 and GFF4418 overlap by 8 nucleotides GFF4418 and GFF4419 are separated by 38 nucleotides
GFF4416 - Methionine ABC transporter substrate-binding protein, at 201,326 to 202,156
GFF4416
GFF4417 - Methionine ABC transporter ATP-binding protein, at 202,193 to 203,209
GFF4417
GFF4418 - Methionine ABC transporter permease protein, at 203,202 to 203,861
GFF4418
GFF4419 - Selenophosphate-dependent tRNA 2-selenouridine synthase, at 203,900 to 204,994
GFF4419
Position (kb)
202
203
204 Strain fitness (log2 ratio)
-1
0
1 at 201.312 kb on - strand at 201.423 kb on + strand, within GFF4416 at 201.483 kb on + strand, within GFF4416 at 201.483 kb on + strand, within GFF4416 at 201.499 kb on + strand, within GFF4416 at 201.550 kb on - strand, within GFF4416 at 201.550 kb on - strand, within GFF4416 at 201.671 kb on + strand, within GFF4416 at 201.785 kb on - strand, within GFF4416 at 201.793 kb on + strand, within GFF4416 at 201.802 kb on + strand, within GFF4416 at 201.803 kb on - strand, within GFF4416 at 202.113 kb on - strand at 202.473 kb on + strand, within GFF4417 at 202.474 kb on - strand, within GFF4417 at 203.083 kb on + strand, within GFF4417 at 203.134 kb on + strand at 203.410 kb on + strand, within GFF4418 at 203.410 kb on + strand, within GFF4418 at 203.423 kb on - strand, within GFF4418 at 203.454 kb on - strand, within GFF4418 at 203.594 kb on + strand, within GFF4418 at 203.595 kb on - strand, within GFF4418 at 203.595 kb on - strand, within GFF4418 at 203.597 kb on + strand, within GFF4418 at 203.597 kb on + strand, within GFF4418 at 203.598 kb on - strand, within GFF4418 at 203.609 kb on - strand, within GFF4418 at 203.668 kb on + strand, within GFF4418 at 203.742 kb on + strand, within GFF4418 at 203.874 kb on + strand at 203.896 kb on + strand at 203.897 kb on - strand at 203.902 kb on - strand at 203.985 kb on + strand at 203.986 kb on - strand at 204.007 kb on + strand at 204.056 kb on + strand, within GFF4419 at 204.162 kb on + strand, within GFF4419
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2 remove 201,312 - +0.2 201,423 + GFF4416 0.12 -0.7 201,483 + GFF4416 0.19 +0.1 201,483 + GFF4416 0.19 +0.7 201,499 + GFF4416 0.21 +0.3 201,550 - GFF4416 0.27 -0.6 201,550 - GFF4416 0.27 -1.3 201,671 + GFF4416 0.42 -0.9 201,785 - GFF4416 0.55 -0.3 201,793 + GFF4416 0.56 +0.3 201,802 + GFF4416 0.57 +0.0 201,803 - GFF4416 0.57 +0.3 202,113 - -0.2 202,473 + GFF4417 0.28 -0.4 202,474 - GFF4417 0.28 -0.6 203,083 + GFF4417 0.88 -1.6 203,134 + +0.1 203,410 + GFF4418 0.32 +0.4 203,410 + GFF4418 0.32 +0.4 203,423 - GFF4418 0.33 -0.0 203,454 - GFF4418 0.38 -0.1 203,594 + GFF4418 0.59 +0.3 203,595 - GFF4418 0.60 -0.2 203,595 - GFF4418 0.60 +1.5 203,597 + GFF4418 0.60 -0.1 203,597 + GFF4418 0.60 +0.6 203,598 - GFF4418 0.60 -0.5 203,609 - GFF4418 0.62 -0.8 203,668 + GFF4418 0.71 -0.0 203,742 + GFF4418 0.82 +0.6 203,874 + -0.9 203,896 + +0.2 203,897 - -0.1 203,902 - -0.2 203,985 + -0.3 203,986 - +0.1 204,007 + -0.4 204,056 + GFF4419 0.14 +1.5 204,162 + GFF4419 0.24 -0.2
Or see this region's nucleotide sequence