Experiment: Acanthamoeba sp., muxed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4333 and GFF4334 are separated by 9 nucleotides GFF4334 and GFF4335 are separated by 96 nucleotides GFF4335 and GFF4336 overlap by 38 nucleotides GFF4336 and GFF4337 are separated by 218 nucleotides
GFF4333 - 2-aminoethylphosphonate:pyruvate aminotransferase (EC 2.6.1.37), at 114,420 to 115,523
GFF4333
GFF4334 - Phosphonoacetaldehyde hydrolase (EC 3.11.1.1), at 115,533 to 116,342
GFF4334
GFF4335 - DJ-1/YajL/PfpI superfamily, includes chaperone protein YajL (former ThiJ), parkinsonism-associated protein DJ-1, peptidases PfpI, Hsp31, at 116,439 to 117,029
GFF4335
GFF4336 - 2-dehydropantoate 2-reductase (EC 1.1.1.169), at 116,992 to 117,810
GFF4336
GFF4337 - FIG001943: hypothetical protein YajQ, at 118,029 to 118,520
GFF4337
Position (kb)
116
117
118 Strain fitness (log2 ratio)
-1
0
1 at 115.459 kb on + strand at 115.459 kb on + strand at 115.459 kb on + strand at 115.459 kb on + strand at 115.460 kb on - strand at 115.509 kb on + strand at 115.521 kb on + strand at 115.553 kb on + strand at 115.718 kb on - strand, within GFF4334 at 115.788 kb on + strand, within GFF4334 at 115.841 kb on + strand, within GFF4334 at 115.895 kb on - strand, within GFF4334 at 116.262 kb on + strand at 116.306 kb on + strand at 116.449 kb on + strand at 116.450 kb on - strand at 116.454 kb on - strand at 116.454 kb on - strand at 116.575 kb on - strand, within GFF4335 at 116.679 kb on - strand, within GFF4335 at 116.679 kb on - strand, within GFF4335 at 116.994 kb on - strand at 116.994 kb on - strand at 117.117 kb on - strand, within GFF4336 at 117.216 kb on - strand, within GFF4336 at 117.303 kb on + strand, within GFF4336 at 117.304 kb on - strand, within GFF4336 at 117.427 kb on + strand, within GFF4336 at 117.489 kb on + strand, within GFF4336 at 117.489 kb on + strand, within GFF4336 at 117.490 kb on - strand, within GFF4336 at 117.495 kb on + strand, within GFF4336 at 117.496 kb on - strand, within GFF4336 at 117.496 kb on - strand, within GFF4336 at 117.526 kb on - strand, within GFF4336 at 117.553 kb on - strand, within GFF4336 at 117.580 kb on - strand, within GFF4336 at 117.612 kb on + strand, within GFF4336 at 117.704 kb on - strand, within GFF4336 at 117.798 kb on - strand at 117.826 kb on + strand at 117.826 kb on + strand at 117.950 kb on + strand at 117.991 kb on + strand at 117.992 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2 remove 115,459 + +0.1 115,459 + +0.1 115,459 + -0.3 115,459 + -0.1 115,460 - +0.1 115,509 + -0.4 115,521 + +0.2 115,553 + +0.2 115,718 - GFF4334 0.23 -0.0 115,788 + GFF4334 0.31 -0.1 115,841 + GFF4334 0.38 -0.1 115,895 - GFF4334 0.45 -0.2 116,262 + +0.3 116,306 + -0.4 116,449 + +0.3 116,450 - +0.4 116,454 - +0.9 116,454 - +0.3 116,575 - GFF4335 0.23 -0.1 116,679 - GFF4335 0.41 +0.5 116,679 - GFF4335 0.41 +0.8 116,994 - -1.0 116,994 - -0.1 117,117 - GFF4336 0.15 -0.4 117,216 - GFF4336 0.27 +0.3 117,303 + GFF4336 0.38 -1.6 117,304 - GFF4336 0.38 -0.4 117,427 + GFF4336 0.53 +0.9 117,489 + GFF4336 0.61 +1.5 117,489 + GFF4336 0.61 -0.2 117,490 - GFF4336 0.61 +0.2 117,495 + GFF4336 0.61 -1.2 117,496 - GFF4336 0.62 -0.5 117,496 - GFF4336 0.62 -0.2 117,526 - GFF4336 0.65 +0.3 117,553 - GFF4336 0.68 -0.0 117,580 - GFF4336 0.72 +0.4 117,612 + GFF4336 0.76 +0.0 117,704 - GFF4336 0.87 +0.1 117,798 - +0.0 117,826 + +0.1 117,826 + -0.6 117,950 + -0.3 117,991 + -0.8 117,992 - -0.2
Or see this region's nucleotide sequence