Experiment: Acanthamoeba sp., muxed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4198 and GFF4199 overlap by 1 nucleotides GFF4199 and GFF4200 overlap by 8 nucleotides GFF4200 and GFF4201 overlap by 20 nucleotides GFF4201 and GFF4202 are separated by 1 nucleotides
GFF4198 - Thiamin biosynthesis protein ThiC, at 12,199 to 14,094
GFF4198
GFF4199 - Thiamin-phosphate pyrophosphorylase (EC 2.5.1.3), at 14,094 to 14,729
GFF4199
GFF4200 - Sulfur carrier protein adenylyltransferase ThiF, at 14,722 to 15,480
GFF4200
GFF4201 - Sulfur carrier protein ThiS, at 15,461 to 15,661
GFF4201
GFF4202 - Thiazole biosynthesis protein ThiG, at 15,663 to 16,433
GFF4202
Position (kb) 14
15 Strain fitness (log2 ratio) -1
0
1 at 13.129 kb on - strand, within GFF4198 at 13.155 kb on - strand, within GFF4198 at 13.216 kb on + strand, within GFF4198 at 13.440 kb on + strand, within GFF4198 at 13.440 kb on + strand, within GFF4198 at 13.521 kb on + strand, within GFF4198 at 13.521 kb on + strand, within GFF4198 at 13.576 kb on + strand, within GFF4198 at 13.682 kb on - strand, within GFF4198 at 14.014 kb on - strand at 14.097 kb on + strand at 14.097 kb on + strand at 14.098 kb on - strand at 14.098 kb on - strand at 14.321 kb on + strand, within GFF4199 at 14.321 kb on + strand, within GFF4199 at 14.322 kb on - strand, within GFF4199 at 14.430 kb on - strand, within GFF4199 at 14.466 kb on + strand, within GFF4199 at 14.467 kb on - strand, within GFF4199 at 14.467 kb on - strand, within GFF4199 at 14.467 kb on - strand, within GFF4199 at 14.549 kb on + strand, within GFF4199 at 14.660 kb on + strand, within GFF4199 at 14.813 kb on + strand, within GFF4200 at 14.813 kb on + strand, within GFF4200 at 14.814 kb on - strand, within GFF4200 at 15.194 kb on + strand, within GFF4200 at 15.194 kb on + strand, within GFF4200 at 15.195 kb on - strand, within GFF4200 at 15.662 kb on + strand at 15.662 kb on + strand at 15.667 kb on + strand at 15.667 kb on + strand at 15.667 kb on + strand at 15.668 kb on - strand at 15.671 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2 remove 13,129 - GFF4198 0.49 -0.5 13,155 - GFF4198 0.50 -0.2 13,216 + GFF4198 0.54 -0.1 13,440 + GFF4198 0.65 +1.7 13,440 + GFF4198 0.65 +0.4 13,521 + GFF4198 0.70 +0.1 13,521 + GFF4198 0.70 -0.4 13,576 + GFF4198 0.73 +0.5 13,682 - GFF4198 0.78 -1.0 14,014 - -0.6 14,097 + +1.7 14,097 + -0.4 14,098 - -0.4 14,098 - -0.3 14,321 + GFF4199 0.36 -0.7 14,321 + GFF4199 0.36 -0.6 14,322 - GFF4199 0.36 -0.2 14,430 - GFF4199 0.53 -0.9 14,466 + GFF4199 0.58 -0.8 14,467 - GFF4199 0.59 -0.1 14,467 - GFF4199 0.59 -1.2 14,467 - GFF4199 0.59 +0.4 14,549 + GFF4199 0.72 -0.6 14,660 + GFF4199 0.89 -0.9 14,813 + GFF4200 0.12 +0.6 14,813 + GFF4200 0.12 +0.1 14,814 - GFF4200 0.12 +0.2 15,194 + GFF4200 0.62 +0.7 15,194 + GFF4200 0.62 +0.0 15,195 - GFF4200 0.62 +0.3 15,662 + -0.3 15,662 + +0.1 15,667 + -0.0 15,667 + -0.1 15,667 + -0.1 15,668 - +0.0 15,671 + -1.0
Or see this region's nucleotide sequence