Experiment: Acanthamoeba sp., muxed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4176 and GFF4177 are separated by 9 nucleotides GFF4177 and GFF4178 are separated by 57 nucleotides GFF4178 and GFF4179 overlap by 40 nucleotides
GFF4176 - 2-keto-3-deoxy-D-arabino-heptulosonate-7- phosphate synthase I alpha (EC 2.5.1.54), at 64,952 to 66,022
GFF4176
GFF4177 - Chorismate mutase I (EC 5.4.99.5) / Cyclohexadienyl dehydrogenase (EC 1.3.1.12)(EC 1.3.1.43), at 66,032 to 67,153
GFF4177
GFF4178 - FIG074102: hypothetical protein, at 67,211 to 68,119
GFF4178
GFF4179 - Chorismate mutase I (EC 5.4.99.5) / Prephenate dehydratase (EC 4.2.1.51), at 68,080 to 69,240
GFF4179
Position (kb) 66
67
68 Strain fitness (log2 ratio) -1
0
1
2 at 65.062 kb on - strand, within GFF4176 at 65.124 kb on + strand, within GFF4176 at 65.124 kb on + strand, within GFF4176 at 65.168 kb on + strand, within GFF4176 at 65.169 kb on - strand, within GFF4176 at 65.216 kb on + strand, within GFF4176 at 65.676 kb on - strand, within GFF4176 at 65.676 kb on - strand, within GFF4176 at 65.728 kb on - strand, within GFF4176 at 65.745 kb on + strand, within GFF4176 at 65.745 kb on + strand, within GFF4176 at 65.821 kb on + strand, within GFF4176 at 65.863 kb on + strand, within GFF4176 at 65.863 kb on + strand, within GFF4176 at 65.863 kb on + strand, within GFF4176 at 65.944 kb on + strand at 65.945 kb on - strand at 65.971 kb on - strand at 66.066 kb on + strand at 66.187 kb on + strand, within GFF4177 at 66.188 kb on - strand, within GFF4177 at 66.188 kb on - strand, within GFF4177 at 66.278 kb on + strand, within GFF4177 at 66.539 kb on - strand, within GFF4177 at 66.938 kb on + strand, within GFF4177 at 66.939 kb on - strand, within GFF4177 at 66.939 kb on - strand, within GFF4177 at 66.949 kb on + strand, within GFF4177 at 66.950 kb on - strand, within GFF4177 at 66.952 kb on + strand, within GFF4177 at 66.992 kb on + strand, within GFF4177 at 66.992 kb on + strand, within GFF4177 at 66.992 kb on + strand, within GFF4177 at 67.165 kb on - strand at 67.167 kb on - strand at 67.167 kb on - strand at 67.170 kb on - strand at 67.216 kb on + strand at 67.230 kb on + strand at 67.292 kb on + strand at 67.351 kb on + strand, within GFF4178 at 67.352 kb on - strand, within GFF4178 at 67.432 kb on + strand, within GFF4178 at 67.432 kb on + strand, within GFF4178 at 67.447 kb on + strand, within GFF4178 at 67.458 kb on + strand, within GFF4178 at 67.492 kb on + strand, within GFF4178 at 67.574 kb on + strand, within GFF4178 at 67.575 kb on - strand, within GFF4178 at 67.686 kb on - strand, within GFF4178 at 67.686 kb on - strand, within GFF4178 at 67.686 kb on - strand, within GFF4178 at 67.687 kb on + strand, within GFF4178 at 67.713 kb on + strand, within GFF4178 at 67.715 kb on + strand, within GFF4178 at 67.715 kb on + strand, within GFF4178 at 67.716 kb on - strand, within GFF4178 at 67.716 kb on - strand, within GFF4178 at 67.826 kb on + strand, within GFF4178 at 67.960 kb on + strand, within GFF4178 at 67.969 kb on + strand, within GFF4178 at 67.969 kb on + strand, within GFF4178
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2 remove 65,062 - GFF4176 0.10 +0.0 65,124 + GFF4176 0.16 +0.2 65,124 + GFF4176 0.16 -1.1 65,168 + GFF4176 0.20 -1.0 65,169 - GFF4176 0.20 +0.1 65,216 + GFF4176 0.25 +1.6 65,676 - GFF4176 0.68 -0.1 65,676 - GFF4176 0.68 -0.6 65,728 - GFF4176 0.72 -0.5 65,745 + GFF4176 0.74 +0.2 65,745 + GFF4176 0.74 -0.1 65,821 + GFF4176 0.81 -0.3 65,863 + GFF4176 0.85 -0.3 65,863 + GFF4176 0.85 -0.4 65,863 + GFF4176 0.85 -0.6 65,944 + -0.6 65,945 - -0.6 65,971 - +0.1 66,066 + -0.0 66,187 + GFF4177 0.14 +0.4 66,188 - GFF4177 0.14 +0.0 66,188 - GFF4177 0.14 +2.0 66,278 + GFF4177 0.22 +0.3 66,539 - GFF4177 0.45 +0.3 66,938 + GFF4177 0.81 -0.0 66,939 - GFF4177 0.81 +1.1 66,939 - GFF4177 0.81 -0.1 66,949 + GFF4177 0.82 +0.5 66,950 - GFF4177 0.82 -0.5 66,952 + GFF4177 0.82 -0.1 66,992 + GFF4177 0.86 +0.2 66,992 + GFF4177 0.86 -0.0 66,992 + GFF4177 0.86 -0.2 67,165 - +0.1 67,167 - -0.1 67,167 - -1.4 67,170 - -0.6 67,216 + +0.0 67,230 + -0.2 67,292 + -0.1 67,351 + GFF4178 0.15 +0.1 67,352 - GFF4178 0.16 -0.2 67,432 + GFF4178 0.24 +0.2 67,432 + GFF4178 0.24 -0.2 67,447 + GFF4178 0.26 +0.3 67,458 + GFF4178 0.27 +0.7 67,492 + GFF4178 0.31 -0.4 67,574 + GFF4178 0.40 +0.2 67,575 - GFF4178 0.40 +0.1 67,686 - GFF4178 0.52 -0.0 67,686 - GFF4178 0.52 -0.1 67,686 - GFF4178 0.52 -0.3 67,687 + GFF4178 0.52 +0.1 67,713 + GFF4178 0.55 +0.3 67,715 + GFF4178 0.55 +0.1 67,715 + GFF4178 0.55 +0.5 67,716 - GFF4178 0.56 -0.1 67,716 - GFF4178 0.56 +1.2 67,826 + GFF4178 0.68 +0.3 67,960 + GFF4178 0.82 -0.0 67,969 + GFF4178 0.83 -0.0 67,969 + GFF4178 0.83 -0.4
Or see this region's nucleotide sequence