Experiment: Acanthamoeba sp., muxed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4175 and GFF4176 are separated by 439 nucleotides GFF4176 and GFF4177 are separated by 9 nucleotides
GFF4175 - Uncharacterized protein YfiR precursor, at 63,994 to 64,512
GFF4175
GFF4176 - 2-keto-3-deoxy-D-arabino-heptulosonate-7- phosphate synthase I alpha (EC 2.5.1.54), at 64,952 to 66,022
GFF4176
GFF4177 - Chorismate mutase I (EC 5.4.99.5) / Cyclohexadienyl dehydrogenase (EC 1.3.1.12)(EC 1.3.1.43), at 66,032 to 67,153
GFF4177
Position (kb) 64
65
66
67 Strain fitness (log2 ratio) -1
0
1
2 at 64.102 kb on - strand, within GFF4175 at 64.156 kb on + strand, within GFF4175 at 64.346 kb on - strand, within GFF4175 at 64.352 kb on - strand, within GFF4175 at 64.386 kb on + strand, within GFF4175 at 64.386 kb on + strand, within GFF4175 at 64.387 kb on - strand, within GFF4175 at 64.388 kb on + strand, within GFF4175 at 64.552 kb on + strand at 64.820 kb on + strand at 64.820 kb on + strand at 64.820 kb on + strand at 64.821 kb on - strand at 64.978 kb on - strand at 64.999 kb on - strand at 65.020 kb on + strand at 65.062 kb on - strand, within GFF4176 at 65.124 kb on + strand, within GFF4176 at 65.124 kb on + strand, within GFF4176 at 65.168 kb on + strand, within GFF4176 at 65.169 kb on - strand, within GFF4176 at 65.216 kb on + strand, within GFF4176 at 65.676 kb on - strand, within GFF4176 at 65.676 kb on - strand, within GFF4176 at 65.728 kb on - strand, within GFF4176 at 65.745 kb on + strand, within GFF4176 at 65.745 kb on + strand, within GFF4176 at 65.821 kb on + strand, within GFF4176 at 65.863 kb on + strand, within GFF4176 at 65.863 kb on + strand, within GFF4176 at 65.863 kb on + strand, within GFF4176 at 65.944 kb on + strand at 65.945 kb on - strand at 65.971 kb on - strand at 66.066 kb on + strand at 66.187 kb on + strand, within GFF4177 at 66.188 kb on - strand, within GFF4177 at 66.188 kb on - strand, within GFF4177 at 66.278 kb on + strand, within GFF4177 at 66.539 kb on - strand, within GFF4177 at 66.938 kb on + strand, within GFF4177 at 66.939 kb on - strand, within GFF4177 at 66.939 kb on - strand, within GFF4177 at 66.949 kb on + strand, within GFF4177 at 66.950 kb on - strand, within GFF4177 at 66.952 kb on + strand, within GFF4177 at 66.992 kb on + strand, within GFF4177 at 66.992 kb on + strand, within GFF4177 at 66.992 kb on + strand, within GFF4177
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2 remove 64,102 - GFF4175 0.21 +0.1 64,156 + GFF4175 0.31 -1.3 64,346 - GFF4175 0.68 +0.2 64,352 - GFF4175 0.69 -0.4 64,386 + GFF4175 0.76 +0.6 64,386 + GFF4175 0.76 -0.2 64,387 - GFF4175 0.76 +0.3 64,388 + GFF4175 0.76 +0.1 64,552 + -0.5 64,820 + +0.0 64,820 + +0.1 64,820 + +0.4 64,821 - +0.1 64,978 - -0.7 64,999 - -0.9 65,020 + +0.1 65,062 - GFF4176 0.10 +0.0 65,124 + GFF4176 0.16 +0.2 65,124 + GFF4176 0.16 -1.1 65,168 + GFF4176 0.20 -1.0 65,169 - GFF4176 0.20 +0.1 65,216 + GFF4176 0.25 +1.6 65,676 - GFF4176 0.68 -0.1 65,676 - GFF4176 0.68 -0.6 65,728 - GFF4176 0.72 -0.5 65,745 + GFF4176 0.74 +0.2 65,745 + GFF4176 0.74 -0.1 65,821 + GFF4176 0.81 -0.3 65,863 + GFF4176 0.85 -0.3 65,863 + GFF4176 0.85 -0.4 65,863 + GFF4176 0.85 -0.6 65,944 + -0.6 65,945 - -0.6 65,971 - +0.1 66,066 + -0.0 66,187 + GFF4177 0.14 +0.4 66,188 - GFF4177 0.14 +0.0 66,188 - GFF4177 0.14 +2.0 66,278 + GFF4177 0.22 +0.3 66,539 - GFF4177 0.45 +0.3 66,938 + GFF4177 0.81 -0.0 66,939 - GFF4177 0.81 +1.1 66,939 - GFF4177 0.81 -0.1 66,949 + GFF4177 0.82 +0.5 66,950 - GFF4177 0.82 -0.5 66,952 + GFF4177 0.82 -0.1 66,992 + GFF4177 0.86 +0.2 66,992 + GFF4177 0.86 -0.0 66,992 + GFF4177 0.86 -0.2
Or see this region's nucleotide sequence