Experiment: Acanthamoeba sp., muxed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4072 and GFF4073 are separated by 23 nucleotides GFF4073 and GFF4074 are separated by 18 nucleotides GFF4074 and GFF4075 overlap by 7 nucleotides GFF4075 and GFF4076 are separated by 192 nucleotides
GFF4072 - VgrG protein, at 35,283 to 37,472
GFF4072
GFF4073 - FIG074102: hypothetical protein, at 37,496 to 37,942
GFF4073
GFF4074 - ClpB protein, at 37,961 to 42,055
GFF4074
GFF4075 - FIG01047741: hypothetical protein, at 42,049 to 42,312
GFF4075
GFF4076 - ClpB protein, at 42,505 to 43,245
GFF4076
Position (kb)
37
38
39
40
41
42
43 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 37.009 kb on - strand, within GFF4072 at 37.318 kb on + strand at 37.318 kb on + strand at 37.363 kb on + strand at 37.363 kb on + strand at 37.508 kb on + strand at 37.613 kb on - strand, within GFF4073 at 37.655 kb on + strand, within GFF4073 at 37.655 kb on + strand, within GFF4073 at 37.802 kb on + strand, within GFF4073 at 37.965 kb on - strand at 37.994 kb on + strand at 38.472 kb on + strand, within GFF4074 at 38.473 kb on - strand, within GFF4074 at 38.555 kb on - strand, within GFF4074 at 38.901 kb on + strand, within GFF4074 at 38.902 kb on - strand, within GFF4074 at 38.903 kb on + strand, within GFF4074 at 38.930 kb on + strand, within GFF4074 at 38.930 kb on + strand, within GFF4074 at 38.943 kb on - strand, within GFF4074 at 38.957 kb on + strand, within GFF4074 at 38.996 kb on + strand, within GFF4074 at 38.997 kb on - strand, within GFF4074 at 39.182 kb on + strand, within GFF4074 at 39.386 kb on + strand, within GFF4074 at 39.449 kb on + strand, within GFF4074 at 39.450 kb on - strand, within GFF4074 at 39.464 kb on + strand, within GFF4074 at 39.465 kb on - strand, within GFF4074 at 39.465 kb on - strand, within GFF4074 at 39.466 kb on + strand, within GFF4074 at 39.512 kb on + strand, within GFF4074 at 39.576 kb on - strand, within GFF4074 at 39.761 kb on + strand, within GFF4074 at 39.887 kb on + strand, within GFF4074 at 39.888 kb on - strand, within GFF4074 at 39.888 kb on - strand, within GFF4074 at 39.945 kb on + strand, within GFF4074 at 39.956 kb on + strand, within GFF4074 at 39.957 kb on - strand, within GFF4074 at 40.014 kb on - strand, within GFF4074 at 40.014 kb on - strand, within GFF4074 at 40.016 kb on - strand, within GFF4074 at 40.016 kb on - strand, within GFF4074 at 40.016 kb on - strand, within GFF4074 at 40.016 kb on - strand, within GFF4074 at 40.016 kb on - strand, within GFF4074 at 40.085 kb on - strand, within GFF4074 at 40.261 kb on + strand, within GFF4074 at 40.287 kb on - strand, within GFF4074 at 40.406 kb on + strand, within GFF4074 at 40.551 kb on + strand, within GFF4074 at 40.551 kb on + strand, within GFF4074 at 40.551 kb on + strand, within GFF4074 at 40.551 kb on + strand, within GFF4074 at 40.551 kb on + strand, within GFF4074 at 40.551 kb on + strand, within GFF4074 at 40.551 kb on + strand, within GFF4074 at 40.551 kb on + strand, within GFF4074 at 40.551 kb on + strand, within GFF4074 at 40.552 kb on - strand, within GFF4074 at 40.552 kb on - strand, within GFF4074 at 40.552 kb on - strand, within GFF4074 at 40.552 kb on - strand, within GFF4074 at 40.552 kb on - strand, within GFF4074 at 40.552 kb on - strand, within GFF4074 at 40.552 kb on - strand, within GFF4074 at 40.552 kb on - strand, within GFF4074 at 40.552 kb on - strand, within GFF4074 at 40.628 kb on + strand, within GFF4074 at 40.711 kb on - strand, within GFF4074 at 40.739 kb on + strand, within GFF4074 at 40.856 kb on + strand, within GFF4074 at 40.856 kb on + strand, within GFF4074 at 40.920 kb on - strand, within GFF4074 at 40.920 kb on - strand, within GFF4074 at 40.926 kb on - strand, within GFF4074 at 40.967 kb on + strand, within GFF4074 at 40.985 kb on + strand, within GFF4074 at 41.080 kb on + strand, within GFF4074 at 41.471 kb on + strand, within GFF4074 at 41.693 kb on + strand at 41.694 kb on - strand at 41.826 kb on + strand at 41.860 kb on + strand at 41.861 kb on - strand at 41.861 kb on - strand at 41.865 kb on - strand at 41.865 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2 remove 37,009 - GFF4072 0.79 +0.2 37,318 + -0.5 37,318 + -0.2 37,363 + -0.4 37,363 + +0.5 37,508 + -0.5 37,613 - GFF4073 0.26 -0.2 37,655 + GFF4073 0.36 +0.7 37,655 + GFF4073 0.36 -0.0 37,802 + GFF4073 0.68 -0.3 37,965 - +0.0 37,994 + -0.0 38,472 + GFF4074 0.12 +0.5 38,473 - GFF4074 0.13 -0.8 38,555 - GFF4074 0.15 -0.1 38,901 + GFF4074 0.23 -0.1 38,902 - GFF4074 0.23 +0.4 38,903 + GFF4074 0.23 -0.4 38,930 + GFF4074 0.24 +0.0 38,930 + GFF4074 0.24 +0.1 38,943 - GFF4074 0.24 -1.8 38,957 + GFF4074 0.24 +0.4 38,996 + GFF4074 0.25 +0.4 38,997 - GFF4074 0.25 -0.5 39,182 + GFF4074 0.30 +0.0 39,386 + GFF4074 0.35 -0.2 39,449 + GFF4074 0.36 -0.4 39,450 - GFF4074 0.36 +0.9 39,464 + GFF4074 0.37 -0.2 39,465 - GFF4074 0.37 +0.1 39,465 - GFF4074 0.37 +0.9 39,466 + GFF4074 0.37 +0.4 39,512 + GFF4074 0.38 +0.4 39,576 - GFF4074 0.39 -0.4 39,761 + GFF4074 0.44 -0.2 39,887 + GFF4074 0.47 +0.1 39,888 - GFF4074 0.47 -1.1 39,888 - GFF4074 0.47 +1.6 39,945 + GFF4074 0.48 +0.0 39,956 + GFF4074 0.49 +0.6 39,957 - GFF4074 0.49 +0.3 40,014 - GFF4074 0.50 +0.8 40,014 - GFF4074 0.50 -0.6 40,016 - GFF4074 0.50 +0.1 40,016 - GFF4074 0.50 +0.2 40,016 - GFF4074 0.50 +0.3 40,016 - GFF4074 0.50 +0.1 40,016 - GFF4074 0.50 -1.6 40,085 - GFF4074 0.52 -0.2 40,261 + GFF4074 0.56 -0.1 40,287 - GFF4074 0.57 -0.1 40,406 + GFF4074 0.60 +0.5 40,551 + GFF4074 0.63 +0.6 40,551 + GFF4074 0.63 +0.0 40,551 + GFF4074 0.63 -0.1 40,551 + GFF4074 0.63 +2.1 40,551 + GFF4074 0.63 +0.1 40,551 + GFF4074 0.63 +0.6 40,551 + GFF4074 0.63 -2.3 40,551 + GFF4074 0.63 -0.1 40,551 + GFF4074 0.63 +0.2 40,552 - GFF4074 0.63 -0.5 40,552 - GFF4074 0.63 -1.0 40,552 - GFF4074 0.63 -0.1 40,552 - GFF4074 0.63 +0.8 40,552 - GFF4074 0.63 -0.3 40,552 - GFF4074 0.63 -0.4 40,552 - GFF4074 0.63 -0.3 40,552 - GFF4074 0.63 -0.4 40,552 - GFF4074 0.63 +0.5 40,628 + GFF4074 0.65 +0.4 40,711 - GFF4074 0.67 -0.2 40,739 + GFF4074 0.68 -1.4 40,856 + GFF4074 0.71 +0.5 40,856 + GFF4074 0.71 +0.3 40,920 - GFF4074 0.72 +1.0 40,920 - GFF4074 0.72 -1.2 40,926 - GFF4074 0.72 +0.1 40,967 + GFF4074 0.73 +0.6 40,985 + GFF4074 0.74 +0.2 41,080 + GFF4074 0.76 +0.8 41,471 + GFF4074 0.86 +0.0 41,693 + -0.1 41,694 - -0.3 41,826 + +0.0 41,860 + -0.1 41,861 - -0.1 41,861 - -0.1 41,865 - -0.6 41,865 - +0.3
Or see this region's nucleotide sequence