Experiment: Acanthamoeba sp., muxed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3782 and GFF3783 are separated by 4 nucleotides GFF3783 and GFF3784 overlap by 12 nucleotides GFF3784 and GFF3785 are separated by 329 nucleotides
GFF3782 - L-serine dehydratase, beta subunit (EC 4.3.1.17) / L-serine dehydratase, alpha subunit (EC 4.3.1.17), at 50,869 to 52,170
GFF3782
GFF3783 - Putative DNA-binding protein in cluster with Type I restriction-modification system, at 52,175 to 52,564
GFF3783
GFF3784 - S-formylglutathione hydrolase (EC 3.1.2.12), at 52,553 to 53,410
GFF3784
GFF3785 - GTP cyclohydrolase I (EC 3.5.4.16) type 1, at 53,740 to 54,408
GFF3785
Position (kb)
52
53
54 Strain fitness (log2 ratio)
-1
0
1 at 51.698 kb on - strand, within GFF3782 at 51.778 kb on - strand, within GFF3782 at 51.792 kb on + strand, within GFF3782 at 51.963 kb on - strand, within GFF3782 at 52.085 kb on - strand at 52.110 kb on + strand at 52.126 kb on + strand at 52.127 kb on - strand at 52.182 kb on - strand at 52.186 kb on + strand at 52.342 kb on + strand, within GFF3783 at 52.343 kb on - strand, within GFF3783 at 52.496 kb on - strand, within GFF3783 at 52.642 kb on + strand, within GFF3784 at 52.643 kb on - strand, within GFF3784 at 52.643 kb on - strand, within GFF3784 at 52.643 kb on - strand, within GFF3784 at 52.851 kb on + strand, within GFF3784 at 52.956 kb on - strand, within GFF3784 at 53.012 kb on - strand, within GFF3784 at 53.012 kb on - strand, within GFF3784 at 53.187 kb on - strand, within GFF3784 at 53.187 kb on - strand, within GFF3784 at 53.272 kb on - strand, within GFF3784 at 53.272 kb on - strand, within GFF3784 at 53.439 kb on + strand at 53.509 kb on + strand at 54.410 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2 remove 51,698 - GFF3782 0.64 +0.1 51,778 - GFF3782 0.70 -0.8 51,792 + GFF3782 0.71 -0.0 51,963 - GFF3782 0.84 +0.8 52,085 - +0.1 52,110 + -0.3 52,126 + +0.4 52,127 - -0.3 52,182 - -0.6 52,186 + +0.0 52,342 + GFF3783 0.43 +0.1 52,343 - GFF3783 0.43 -1.0 52,496 - GFF3783 0.82 +0.0 52,642 + GFF3784 0.10 +0.0 52,643 - GFF3784 0.10 -0.5 52,643 - GFF3784 0.10 -0.7 52,643 - GFF3784 0.10 -0.1 52,851 + GFF3784 0.35 -0.1 52,956 - GFF3784 0.47 -0.4 53,012 - GFF3784 0.53 +0.2 53,012 - GFF3784 0.53 -0.9 53,187 - GFF3784 0.74 -0.7 53,187 - GFF3784 0.74 +0.3 53,272 - GFF3784 0.84 -0.1 53,272 - GFF3784 0.84 +0.1 53,439 + -0.1 53,509 + +0.5 54,410 + -1.1
Or see this region's nucleotide sequence