Experiment: Acanthamoeba sp., muxed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3595 and GFF3596 are separated by 93 nucleotides GFF3596 and GFF3597 overlap by 23 nucleotides GFF3597 and GFF3598 overlap by 4 nucleotides GFF3598 and GFF3599 are separated by 56 nucleotides GFF3599 and GFF3600 are separated by 17 nucleotides GFF3600 and GFF3601 overlap by 4 nucleotides
GFF3595 - Secretion system effector SseG, at 350,909 to 351,496
GFF3595
GFF3596 - Type III secretion protein SsaG, at 351,590 to 351,805
GFF3596
GFF3597 - Type III secretion protein SsaH, at 351,783 to 352,073
GFF3597
GFF3598 - Type III secretion protein SsaI, at 352,070 to 352,333
GFF3598
GFF3599 - Type III secretion bridge between inner and outermembrane lipoprotein (YscJ,HrcJ,EscJ, PscJ), at 352,390 to 353,079
GFF3599
GFF3600 - FIG029138: Type III secretion protein, at 353,097 to 353,645
GFF3600
GFF3601 - Type III secretion protein SsaK, at 353,642 to 354,316
GFF3601
Position (kb) 352
353
354 Strain fitness (log2 ratio) -1
0
1
2 at 352.349 kb on + strand at 352.366 kb on - strand at 352.494 kb on - strand, within GFF3599 at 352.608 kb on - strand, within GFF3599 at 352.990 kb on + strand, within GFF3599 at 353.467 kb on + strand, within GFF3600 at 353.937 kb on + strand, within GFF3601 at 354.003 kb on + strand, within GFF3601 at 354.053 kb on - strand, within GFF3601
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2 remove 352,349 + +0.0 352,366 - +0.8 352,494 - GFF3599 0.15 +2.2 352,608 - GFF3599 0.32 +0.2 352,990 + GFF3599 0.87 +0.2 353,467 + GFF3600 0.67 +0.6 353,937 + GFF3601 0.44 +0.2 354,003 + GFF3601 0.53 +0.2 354,053 - GFF3601 0.61 -0.3
Or see this region's nucleotide sequence