Experiment: Acanthamoeba sp., muxed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3557 and GFF3558 are separated by 426 nucleotides GFF3558 and GFF3559 are separated by 8 nucleotides GFF3559 and GFF3560 are separated by 16 nucleotides GFF3560 and GFF3561 overlap by 26 nucleotides
GFF3557 - L-cystine uptake protein TcyP, at 312,595 to 313,941
GFF3557
GFF3558 - Iron binding protein SufA for iron-sulfur cluster assembly, at 314,368 to 314,736
GFF3558
GFF3559 - Iron-sulfur cluster assembly protein SufB, at 314,745 to 316,232
GFF3559
GFF3560 - Iron-sulfur cluster assembly ATPase protein SufC, at 316,249 to 316,995
GFF3560
GFF3561 - Iron-sulfur cluster assembly protein SufD, at 316,970 to 318,241
GFF3561
Position (kb) 314
315
316
317 Strain fitness (log2 ratio) -1
0
1
2 at 313.933 kb on - strand at 313.942 kb on - strand at 314.001 kb on - strand at 314.020 kb on - strand at 314.334 kb on + strand at 314.334 kb on + strand at 314.334 kb on + strand at 314.336 kb on + strand at 314.451 kb on - strand, within GFF3558 at 314.734 kb on + strand at 314.735 kb on - strand at 314.741 kb on + strand at 314.744 kb on + strand at 314.744 kb on + strand at 314.937 kb on - strand, within GFF3559 at 314.937 kb on - strand, within GFF3559 at 315.012 kb on + strand, within GFF3559 at 315.047 kb on + strand, within GFF3559 at 315.048 kb on - strand, within GFF3559 at 315.073 kb on + strand, within GFF3559 at 315.236 kb on + strand, within GFF3559 at 315.291 kb on + strand, within GFF3559 at 315.368 kb on + strand, within GFF3559 at 315.368 kb on + strand, within GFF3559 at 315.368 kb on + strand, within GFF3559 at 315.368 kb on + strand, within GFF3559 at 315.369 kb on - strand, within GFF3559 at 315.369 kb on - strand, within GFF3559 at 315.373 kb on - strand, within GFF3559 at 315.425 kb on + strand, within GFF3559 at 315.484 kb on - strand, within GFF3559 at 315.484 kb on - strand, within GFF3559 at 315.500 kb on - strand, within GFF3559 at 315.529 kb on - strand, within GFF3559 at 315.529 kb on - strand, within GFF3559 at 315.567 kb on - strand, within GFF3559 at 316.022 kb on + strand, within GFF3559 at 316.022 kb on + strand, within GFF3559 at 316.260 kb on + strand at 316.440 kb on + strand, within GFF3560 at 316.658 kb on + strand, within GFF3560 at 317.067 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2 remove 313,933 - +0.4 313,942 - +0.5 314,001 - +0.3 314,020 - +0.5 314,334 + -0.4 314,334 + +0.4 314,334 + +1.3 314,336 + -0.0 314,451 - GFF3558 0.22 +0.0 314,734 + +0.2 314,735 - -0.9 314,741 + -0.1 314,744 + -0.0 314,744 + +1.9 314,937 - GFF3559 0.13 +0.0 314,937 - GFF3559 0.13 +0.9 315,012 + GFF3559 0.18 -0.6 315,047 + GFF3559 0.20 +0.2 315,048 - GFF3559 0.20 +0.5 315,073 + GFF3559 0.22 -0.2 315,236 + GFF3559 0.33 +0.7 315,291 + GFF3559 0.37 -1.2 315,368 + GFF3559 0.42 +0.3 315,368 + GFF3559 0.42 -0.4 315,368 + GFF3559 0.42 -0.5 315,368 + GFF3559 0.42 +0.1 315,369 - GFF3559 0.42 -0.6 315,369 - GFF3559 0.42 +1.0 315,373 - GFF3559 0.42 -0.4 315,425 + GFF3559 0.46 -0.3 315,484 - GFF3559 0.50 -0.8 315,484 - GFF3559 0.50 +0.3 315,500 - GFF3559 0.51 +0.7 315,529 - GFF3559 0.53 -0.5 315,529 - GFF3559 0.53 +0.8 315,567 - GFF3559 0.55 +0.5 316,022 + GFF3559 0.86 +0.5 316,022 + GFF3559 0.86 -0.3 316,260 + -0.5 316,440 + GFF3560 0.26 +0.2 316,658 + GFF3560 0.55 +0.7 317,067 + +0.4
Or see this region's nucleotide sequence