Experiment: Acanthamoeba sp., muxed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3527 and GFF3528 are separated by 4 nucleotides GFF3528 and GFF3529 are separated by 202 nucleotides GFF3529 and GFF3530 are separated by 91 nucleotides GFF3530 and GFF3531 are separated by 11 nucleotides
GFF3527 - Phenylalanyl-tRNA synthetase beta chain (EC 6.1.1.20), at 278,730 to 281,117
GFF3527
GFF3528 - Integration host factor alpha subunit, at 281,122 to 281,421
GFF3528
GFF3529 - Vitamin B12 ABC transporter, permease component BtuC, at 281,624 to 282,604
GFF3529
GFF3530 - Glutathione peroxidase family protein, at 282,696 to 283,247
GFF3530
GFF3531 - Vitamin B12 ABC transporter, ATPase component BtuD, at 283,259 to 283,996
GFF3531
Position (kb) 281
282
283 Strain fitness (log2 ratio) -1
0
1 at 281.272 kb on - strand, within GFF3528 at 281.331 kb on + strand, within GFF3528 at 281.337 kb on + strand, within GFF3528 at 281.452 kb on + strand at 281.452 kb on + strand at 281.464 kb on + strand at 281.465 kb on - strand at 281.484 kb on - strand at 281.504 kb on + strand at 281.504 kb on + strand at 281.505 kb on - strand at 281.715 kb on + strand at 282.007 kb on + strand, within GFF3529 at 282.086 kb on - strand, within GFF3529 at 282.152 kb on + strand, within GFF3529 at 282.421 kb on - strand, within GFF3529 at 282.487 kb on - strand, within GFF3529 at 282.642 kb on - strand at 282.648 kb on - strand at 282.750 kb on - strand at 283.245 kb on + strand at 283.371 kb on + strand, within GFF3531 at 283.371 kb on + strand, within GFF3531 at 283.382 kb on - strand, within GFF3531
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2 remove 281,272 - GFF3528 0.50 -1.3 281,331 + GFF3528 0.70 -1.4 281,337 + GFF3528 0.72 -0.2 281,452 + +0.3 281,452 + +0.7 281,464 + +0.5 281,465 - +0.3 281,484 - +1.3 281,504 + +0.5 281,504 + +0.6 281,505 - -0.2 281,715 + -0.1 282,007 + GFF3529 0.39 +1.1 282,086 - GFF3529 0.47 +0.8 282,152 + GFF3529 0.54 -0.8 282,421 - GFF3529 0.81 -0.2 282,487 - GFF3529 0.88 -0.0 282,642 - -0.5 282,648 - +0.0 282,750 - -0.0 283,245 + +0.3 283,371 + GFF3531 0.15 +1.2 283,371 + GFF3531 0.15 -0.2 283,382 - GFF3531 0.17 -0.5
Or see this region's nucleotide sequence