Experiment: Acanthamoeba sp., muxed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3646 and GFF3312 are separated by 73 nucleotides GFF3312 and GFF3313 overlap by 2 nucleotides GFF3313 and GFF3314 are separated by 83 nucleotides GFF3314 and GFF3315 are separated by 23 nucleotides
GFF3646 - tRNA-Ser-GGA, at 86,793 to 86,880
GFF3646
GFF3312 - FIG01046704: hypothetical protein, at 86,954 to 87,118
GFF3312
GFF3313 - D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95), at 87,117 to 88,055
GFF3313
GFF3314 - Putative hydrolase YcdX (EC 3.1.-.-), at 88,139 to 88,876
GFF3314
GFF3315 - Putative oxidoreductase component of anaerobic dehydrogenases; Functional role page for Chaperone protein TorD, at 88,900 to 89,454
GFF3315
Position (kb)
87
88
89 Strain fitness (log2 ratio)
-1
0
1 at 86.788 kb on - strand at 87.222 kb on - strand, within GFF3313 at 87.286 kb on + strand, within GFF3313 at 87.337 kb on + strand, within GFF3313 at 87.420 kb on + strand, within GFF3313 at 87.420 kb on + strand, within GFF3313 at 87.421 kb on - strand, within GFF3313 at 87.421 kb on - strand, within GFF3313 at 87.440 kb on - strand, within GFF3313 at 87.652 kb on + strand, within GFF3313 at 87.743 kb on + strand, within GFF3313 at 87.851 kb on - strand, within GFF3313 at 88.056 kb on + strand at 88.056 kb on + strand at 88.057 kb on - strand at 88.057 kb on - strand at 88.100 kb on - strand at 88.160 kb on - strand at 88.166 kb on - strand at 88.188 kb on - strand at 88.193 kb on - strand at 88.205 kb on + strand at 88.207 kb on + strand at 88.521 kb on + strand, within GFF3314 at 88.521 kb on + strand, within GFF3314 at 88.522 kb on - strand, within GFF3314 at 88.522 kb on - strand, within GFF3314 at 88.526 kb on - strand, within GFF3314 at 88.526 kb on - strand, within GFF3314 at 88.562 kb on - strand, within GFF3314 at 88.874 kb on + strand at 88.875 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2 remove 86,788 - +0.7 87,222 - GFF3313 0.11 -1.3 87,286 + GFF3313 0.18 -1.7 87,337 + GFF3313 0.23 +0.2 87,420 + GFF3313 0.32 +0.0 87,420 + GFF3313 0.32 -0.4 87,421 - GFF3313 0.32 -0.1 87,421 - GFF3313 0.32 -0.3 87,440 - GFF3313 0.34 -0.5 87,652 + GFF3313 0.57 -0.0 87,743 + GFF3313 0.67 +0.1 87,851 - GFF3313 0.78 -0.3 88,056 + +0.3 88,056 + -0.8 88,057 - +0.3 88,057 - -0.0 88,100 - -0.3 88,160 - +0.0 88,166 - -0.3 88,188 - +0.2 88,193 - +1.0 88,205 + -0.1 88,207 + -1.0 88,521 + GFF3314 0.52 +0.7 88,521 + GFF3314 0.52 -0.0 88,522 - GFF3314 0.52 +0.3 88,522 - GFF3314 0.52 -1.2 88,526 - GFF3314 0.52 +0.6 88,526 - GFF3314 0.52 +0.2 88,562 - GFF3314 0.57 +0.4 88,874 + +1.0 88,875 - -0.5
Or see this region's nucleotide sequence