Experiment: Acanthamoeba sp., muxed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3179 and GFF3180 are separated by 234 nucleotides GFF3180 and GFF3181 overlap by 4 nucleotides GFF3181 and GFF3182 are separated by 387 nucleotides
GFF3179 - iron-chelator utilization protein, at 184,294 to 185,061
GFF3179
GFF3180 - Transcriptional regulator, PadR family, at 185,296 to 185,940
GFF3180
GFF3181 - Methyl-accepting chemotaxis protein I (serine chemoreceptor protein), at 185,937 to 187,502
GFF3181
GFF3182 - Aerotaxis sensor receptor protein, at 187,890 to 189,410
GFF3182
Position (kb) 185
186
187
188 Strain fitness (log2 ratio) -1
0
1 at 184.975 kb on - strand, within GFF3179 at 184.977 kb on + strand, within GFF3179 at 184.978 kb on - strand, within GFF3179 at 184.978 kb on - strand, within GFF3179 at 184.978 kb on - strand, within GFF3179 at 185.044 kb on + strand at 185.337 kb on + strand at 185.552 kb on - strand, within GFF3180 at 185.652 kb on + strand, within GFF3180 at 185.823 kb on + strand, within GFF3180 at 185.997 kb on + strand at 186.108 kb on - strand, within GFF3181 at 186.108 kb on - strand, within GFF3181 at 186.150 kb on + strand, within GFF3181 at 186.189 kb on - strand, within GFF3181 at 186.549 kb on - strand, within GFF3181 at 186.762 kb on + strand, within GFF3181 at 186.763 kb on - strand, within GFF3181 at 186.794 kb on + strand, within GFF3181 at 186.876 kb on - strand, within GFF3181 at 186.877 kb on + strand, within GFF3181 at 186.877 kb on + strand, within GFF3181 at 186.878 kb on - strand, within GFF3181 at 186.878 kb on - strand, within GFF3181 at 186.879 kb on - strand, within GFF3181 at 186.927 kb on + strand, within GFF3181 at 186.927 kb on + strand, within GFF3181 at 186.948 kb on - strand, within GFF3181 at 187.025 kb on + strand, within GFF3181 at 187.097 kb on + strand, within GFF3181 at 187.221 kb on - strand, within GFF3181 at 187.230 kb on + strand, within GFF3181 at 187.370 kb on + strand at 187.371 kb on - strand at 187.661 kb on - strand at 187.663 kb on + strand at 187.794 kb on + strand at 187.875 kb on + strand at 187.875 kb on + strand at 187.885 kb on + strand at 188.066 kb on + strand, within GFF3182 at 188.103 kb on + strand, within GFF3182 at 188.337 kb on - strand, within GFF3182 at 188.337 kb on - strand, within GFF3182 at 188.341 kb on - strand, within GFF3182 at 188.369 kb on + strand, within GFF3182
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2 remove 184,975 - GFF3179 0.89 -0.9 184,977 + GFF3179 0.89 +0.5 184,978 - GFF3179 0.89 +0.9 184,978 - GFF3179 0.89 +0.3 184,978 - GFF3179 0.89 +0.3 185,044 + +0.2 185,337 + +0.1 185,552 - GFF3180 0.40 -0.6 185,652 + GFF3180 0.55 +0.3 185,823 + GFF3180 0.82 -0.4 185,997 + +0.5 186,108 - GFF3181 0.11 +0.1 186,108 - GFF3181 0.11 +0.1 186,150 + GFF3181 0.14 -0.1 186,189 - GFF3181 0.16 +0.9 186,549 - GFF3181 0.39 -0.5 186,762 + GFF3181 0.53 +0.7 186,763 - GFF3181 0.53 +0.0 186,794 + GFF3181 0.55 -0.0 186,876 - GFF3181 0.60 +0.1 186,877 + GFF3181 0.60 -0.5 186,877 + GFF3181 0.60 +0.2 186,878 - GFF3181 0.60 -0.1 186,878 - GFF3181 0.60 +0.3 186,879 - GFF3181 0.60 +0.7 186,927 + GFF3181 0.63 +1.1 186,927 + GFF3181 0.63 -0.2 186,948 - GFF3181 0.65 -0.2 187,025 + GFF3181 0.69 +0.1 187,097 + GFF3181 0.74 +0.5 187,221 - GFF3181 0.82 -0.1 187,230 + GFF3181 0.83 +0.3 187,370 + -0.4 187,371 - -0.2 187,661 - -0.2 187,663 + -0.7 187,794 + +0.2 187,875 + -0.4 187,875 + +0.4 187,885 + -0.5 188,066 + GFF3182 0.12 +0.1 188,103 + GFF3182 0.14 +0.3 188,337 - GFF3182 0.29 +0.3 188,337 - GFF3182 0.29 +0.2 188,341 - GFF3182 0.30 +0.0 188,369 + GFF3182 0.31 +0.4
Or see this region's nucleotide sequence