Experiment: Acanthamoeba sp., muxed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3054 and GFF3055 are separated by 18 nucleotides GFF3055 and GFF3056 overlap by 4 nucleotides GFF3056 and GFF3057 are separated by 7 nucleotides GFF3057 and GFF3058 overlap by 8 nucleotides
GFF3054 - Hypothetical protein YggS, proline synthase co-transcribed bacterial homolog PROSC, at 66,079 to 66,783
GFF3054
GFF3055 - Integral membrane protein YggT, involved in response to extracytoplasmic stress (osmotic shock), at 66,802 to 67,368
GFF3055
GFF3056 - COG1872, at 67,365 to 67,655
GFF3056
GFF3057 - Nucleoside 5-triphosphatase RdgB (dHAPTP, dITP, XTP-specific) (EC 3.6.1.15), at 67,663 to 68,256
GFF3057
GFF3058 - Radical SAM family enzyme, similar to coproporphyrinogen III oxidase, oxygen-independent, clustered with nucleoside-triphosphatase RdgB, at 68,249 to 69,385
GFF3058
Position (kb) 67
68
69 Strain fitness (log2 ratio) -2
-1
0
1 at 66.677 kb on + strand, within GFF3054 at 66.678 kb on - strand, within GFF3054 at 66.687 kb on + strand, within GFF3054 at 66.723 kb on + strand at 66.844 kb on + strand at 66.844 kb on + strand at 66.844 kb on + strand at 66.846 kb on + strand at 66.857 kb on + strand at 67.017 kb on + strand, within GFF3055 at 67.089 kb on + strand, within GFF3055 at 67.089 kb on + strand, within GFF3055 at 67.098 kb on + strand, within GFF3055 at 67.183 kb on + strand, within GFF3055 at 67.183 kb on + strand, within GFF3055 at 67.184 kb on - strand, within GFF3055 at 67.187 kb on + strand, within GFF3055 at 67.187 kb on + strand, within GFF3055 at 67.188 kb on - strand, within GFF3055 at 67.288 kb on + strand, within GFF3055 at 67.289 kb on - strand, within GFF3055 at 67.289 kb on - strand, within GFF3055 at 67.300 kb on - strand, within GFF3055 at 67.319 kb on + strand at 67.319 kb on + strand at 67.439 kb on + strand, within GFF3056 at 67.653 kb on + strand at 67.657 kb on + strand at 67.912 kb on + strand, within GFF3057 at 67.913 kb on - strand, within GFF3057 at 67.924 kb on + strand, within GFF3057 at 67.980 kb on - strand, within GFF3057 at 68.148 kb on - strand, within GFF3057 at 68.255 kb on - strand at 68.278 kb on + strand at 68.278 kb on + strand at 68.285 kb on - strand at 68.806 kb on + strand, within GFF3058 at 68.929 kb on - strand, within GFF3058 at 68.931 kb on + strand, within GFF3058 at 69.008 kb on + strand, within GFF3058 at 69.118 kb on + strand, within GFF3058 at 69.119 kb on - strand, within GFF3058
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2 remove 66,677 + GFF3054 0.85 +0.4 66,678 - GFF3054 0.85 -0.1 66,687 + GFF3054 0.86 -1.0 66,723 + -0.3 66,844 + -0.5 66,844 + +1.0 66,844 + +0.6 66,846 + +0.2 66,857 + +0.4 67,017 + GFF3055 0.38 -0.2 67,089 + GFF3055 0.51 +0.4 67,089 + GFF3055 0.51 -0.6 67,098 + GFF3055 0.52 -0.1 67,183 + GFF3055 0.67 -0.0 67,183 + GFF3055 0.67 +0.6 67,184 - GFF3055 0.67 -0.6 67,187 + GFF3055 0.68 -0.5 67,187 + GFF3055 0.68 -0.0 67,188 - GFF3055 0.68 -0.6 67,288 + GFF3055 0.86 +0.5 67,289 - GFF3055 0.86 -1.0 67,289 - GFF3055 0.86 -2.1 67,300 - GFF3055 0.88 -1.0 67,319 + +0.3 67,319 + +0.4 67,439 + GFF3056 0.25 +0.2 67,653 + +0.4 67,657 + -0.6 67,912 + GFF3057 0.42 -0.4 67,913 - GFF3057 0.42 -0.8 67,924 + GFF3057 0.44 -0.9 67,980 - GFF3057 0.53 -0.9 68,148 - GFF3057 0.82 -0.7 68,255 - -0.0 68,278 + -0.4 68,278 + -0.6 68,285 - +0.3 68,806 + GFF3058 0.49 +1.3 68,929 - GFF3058 0.60 -0.1 68,931 + GFF3058 0.60 +0.7 69,008 + GFF3058 0.67 +0.0 69,118 + GFF3058 0.76 +0.3 69,119 - GFF3058 0.77 -0.3
Or see this region's nucleotide sequence