Experiment: Acanthamoeba sp., muxed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2989 and GFF2990 are separated by 5 nucleotides GFF2990 and GFF2991 are separated by 23 nucleotides
GFF2989 - Single-stranded-DNA-specific exonuclease RecJ (EC 3.1.-.-), at 7,083 to 8,816
GFF2989
GFF2990 - Thiol:disulfide interchange protein DsbC, at 8,822 to 9,535
GFF2990
GFF2991 - Tyrosine recombinase XerD, at 9,559 to 10,455
GFF2991
Position (kb)
8
9
10 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 7.919 kb on + strand, within GFF2989 at 8.024 kb on + strand, within GFF2989 at 8.081 kb on + strand, within GFF2989 at 8.258 kb on + strand, within GFF2989 at 8.467 kb on - strand, within GFF2989 at 8.504 kb on - strand, within GFF2989 at 8.730 kb on + strand at 8.742 kb on + strand at 8.742 kb on + strand at 8.742 kb on + strand at 8.824 kb on - strand at 8.824 kb on - strand at 8.824 kb on - strand at 8.901 kb on + strand, within GFF2990 at 8.902 kb on - strand, within GFF2990 at 8.916 kb on - strand, within GFF2990 at 8.961 kb on + strand, within GFF2990 at 9.007 kb on + strand, within GFF2990 at 9.058 kb on + strand, within GFF2990 at 9.068 kb on - strand, within GFF2990 at 9.114 kb on + strand, within GFF2990 at 9.139 kb on + strand, within GFF2990 at 9.161 kb on + strand, within GFF2990 at 9.161 kb on + strand, within GFF2990 at 9.162 kb on - strand, within GFF2990 at 9.162 kb on - strand, within GFF2990 at 9.231 kb on + strand, within GFF2990 at 9.337 kb on + strand, within GFF2990 at 9.337 kb on + strand, within GFF2990 at 9.338 kb on - strand, within GFF2990 at 9.361 kb on - strand, within GFF2990 at 9.718 kb on + strand, within GFF2991 at 9.719 kb on - strand, within GFF2991 at 9.719 kb on - strand, within GFF2991 at 9.767 kb on + strand, within GFF2991 at 9.856 kb on + strand, within GFF2991 at 10.028 kb on + strand, within GFF2991 at 10.044 kb on + strand, within GFF2991 at 10.045 kb on - strand, within GFF2991 at 10.491 kb on + strand at 10.492 kb on - strand at 10.492 kb on - strand at 10.492 kb on - strand at 10.492 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2 remove 7,919 + GFF2989 0.48 -0.6 8,024 + GFF2989 0.54 -0.3 8,081 + GFF2989 0.58 -2.1 8,258 + GFF2989 0.68 -1.5 8,467 - GFF2989 0.80 -0.6 8,504 - GFF2989 0.82 -0.9 8,730 + -0.2 8,742 + -0.1 8,742 + -0.5 8,742 + -0.6 8,824 - -0.3 8,824 - -0.1 8,824 - +0.2 8,901 + GFF2990 0.11 -2.2 8,902 - GFF2990 0.11 +0.5 8,916 - GFF2990 0.13 +0.5 8,961 + GFF2990 0.19 +0.7 9,007 + GFF2990 0.26 +0.4 9,058 + GFF2990 0.33 +1.7 9,068 - GFF2990 0.34 -0.1 9,114 + GFF2990 0.41 -0.5 9,139 + GFF2990 0.44 -0.0 9,161 + GFF2990 0.47 -0.1 9,161 + GFF2990 0.47 -1.4 9,162 - GFF2990 0.48 -0.9 9,162 - GFF2990 0.48 +0.0 9,231 + GFF2990 0.57 -1.6 9,337 + GFF2990 0.72 -0.1 9,337 + GFF2990 0.72 -0.1 9,338 - GFF2990 0.72 -0.1 9,361 - GFF2990 0.75 +0.2 9,718 + GFF2991 0.18 -2.2 9,719 - GFF2991 0.18 -2.9 9,719 - GFF2991 0.18 -2.0 9,767 + GFF2991 0.23 -2.1 9,856 + GFF2991 0.33 -0.3 10,028 + GFF2991 0.52 -0.3 10,044 + GFF2991 0.54 -2.6 10,045 - GFF2991 0.54 -1.3 10,491 + +0.2 10,492 - -0.1 10,492 - +0.2 10,492 - -1.6 10,492 - -0.2
Or see this region's nucleotide sequence