Experiment: Acanthamoeba sp., muxed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2955 and GFF2956 are separated by 671 nucleotides GFF2956 and GFF2957 are separated by 9 nucleotides
GFF2955 - COG1242: Predicted Fe-S oxidoreductase, at 34,398 to 35,327
GFF2955
GFF2956 - Glutamate synthase [NADPH] large chain (EC 1.4.1.13), at 35,999 to 40,459
GFF2956
GFF2957 - Glutamate synthase [NADPH] small chain (EC 1.4.1.13), at 40,469 to 41,887
GFF2957
Position (kb)
35
36
37
38
39
40
41 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 35.026 kb on + strand, within GFF2955 at 35.027 kb on - strand, within GFF2955 at 35.080 kb on + strand, within GFF2955 at 35.234 kb on + strand, within GFF2955 at 35.258 kb on + strand at 35.304 kb on - strand at 35.310 kb on + strand at 35.533 kb on + strand at 35.622 kb on + strand at 35.659 kb on - strand at 35.659 kb on - strand at 35.836 kb on - strand at 35.901 kb on + strand at 35.907 kb on + strand at 35.998 kb on + strand at 35.999 kb on - strand at 35.999 kb on - strand at 36.006 kb on - strand at 36.029 kb on - strand at 36.057 kb on + strand at 36.057 kb on + strand at 36.057 kb on + strand at 36.058 kb on - strand at 36.058 kb on - strand at 36.058 kb on - strand at 36.058 kb on - strand at 36.175 kb on - strand at 36.265 kb on + strand at 36.525 kb on + strand, within GFF2956 at 36.525 kb on + strand, within GFF2956 at 36.526 kb on - strand, within GFF2956 at 36.526 kb on - strand, within GFF2956 at 36.526 kb on - strand, within GFF2956 at 36.555 kb on - strand, within GFF2956 at 36.569 kb on - strand, within GFF2956 at 36.761 kb on - strand, within GFF2956 at 36.765 kb on - strand, within GFF2956 at 36.767 kb on - strand, within GFF2956 at 36.985 kb on + strand, within GFF2956 at 37.046 kb on - strand, within GFF2956 at 37.079 kb on + strand, within GFF2956 at 37.096 kb on + strand, within GFF2956 at 37.096 kb on + strand, within GFF2956 at 37.096 kb on + strand, within GFF2956 at 37.097 kb on - strand, within GFF2956 at 37.127 kb on - strand, within GFF2956 at 37.213 kb on + strand, within GFF2956 at 37.214 kb on - strand, within GFF2956 at 37.214 kb on - strand, within GFF2956 at 37.262 kb on + strand, within GFF2956 at 37.262 kb on + strand, within GFF2956 at 37.263 kb on - strand, within GFF2956 at 37.382 kb on - strand, within GFF2956 at 37.674 kb on - strand, within GFF2956 at 37.674 kb on - strand, within GFF2956 at 37.849 kb on + strand, within GFF2956 at 37.950 kb on + strand, within GFF2956 at 37.950 kb on + strand, within GFF2956 at 37.951 kb on - strand, within GFF2956 at 38.014 kb on + strand, within GFF2956 at 38.018 kb on + strand, within GFF2956 at 38.129 kb on + strand, within GFF2956 at 38.137 kb on + strand, within GFF2956 at 38.138 kb on - strand, within GFF2956 at 38.138 kb on - strand, within GFF2956 at 38.172 kb on - strand, within GFF2956 at 38.354 kb on + strand, within GFF2956 at 38.355 kb on - strand, within GFF2956 at 38.419 kb on - strand, within GFF2956 at 38.506 kb on - strand, within GFF2956 at 38.564 kb on - strand, within GFF2956 at 38.601 kb on + strand, within GFF2956 at 38.927 kb on - strand, within GFF2956 at 38.937 kb on + strand, within GFF2956 at 38.937 kb on + strand, within GFF2956 at 39.048 kb on - strand, within GFF2956 at 39.304 kb on + strand, within GFF2956 at 39.414 kb on - strand, within GFF2956 at 39.610 kb on + strand, within GFF2956 at 39.750 kb on - strand, within GFF2956 at 39.861 kb on + strand, within GFF2956 at 39.861 kb on + strand, within GFF2956 at 39.861 kb on + strand, within GFF2956 at 39.862 kb on - strand, within GFF2956 at 39.862 kb on - strand, within GFF2956 at 39.862 kb on - strand, within GFF2956 at 39.862 kb on - strand, within GFF2956 at 39.863 kb on + strand, within GFF2956 at 39.864 kb on - strand, within GFF2956 at 39.887 kb on + strand, within GFF2956 at 39.888 kb on - strand, within GFF2956 at 39.990 kb on - strand, within GFF2956 at 40.103 kb on + strand at 40.103 kb on + strand at 40.104 kb on - strand at 40.181 kb on + strand at 40.233 kb on + strand at 40.234 kb on - strand at 40.240 kb on - strand at 40.240 kb on - strand at 40.309 kb on + strand at 40.420 kb on + strand at 40.654 kb on + strand, within GFF2957 at 40.654 kb on + strand, within GFF2957 at 40.654 kb on + strand, within GFF2957 at 40.655 kb on - strand, within GFF2957 at 40.655 kb on - strand, within GFF2957 at 40.655 kb on - strand, within GFF2957 at 40.655 kb on - strand, within GFF2957 at 40.658 kb on + strand, within GFF2957 at 40.658 kb on + strand, within GFF2957 at 40.658 kb on + strand, within GFF2957 at 40.659 kb on - strand, within GFF2957 at 40.659 kb on - strand, within GFF2957 at 40.659 kb on - strand, within GFF2957 at 41.113 kb on + strand, within GFF2957 at 41.136 kb on + strand, within GFF2957 at 41.173 kb on - strand, within GFF2957 at 41.311 kb on + strand, within GFF2957 at 41.312 kb on + strand, within GFF2957 at 41.312 kb on + strand, within GFF2957 at 41.313 kb on - strand, within GFF2957 at 41.313 kb on - strand, within GFF2957 at 41.313 kb on - strand, within GFF2957 at 41.410 kb on - strand, within GFF2957 at 41.410 kb on - strand, within GFF2957
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2 remove 35,026 + GFF2955 0.68 +0.3 35,027 - GFF2955 0.68 +0.4 35,080 + GFF2955 0.73 +0.4 35,234 + GFF2955 0.90 -1.2 35,258 + -0.5 35,304 - -0.1 35,310 + +1.0 35,533 + +1.0 35,622 + +0.4 35,659 - +0.2 35,659 - +0.0 35,836 - -0.2 35,901 + -0.4 35,907 + +1.1 35,998 + -2.0 35,999 - +0.2 35,999 - +0.9 36,006 - +0.7 36,029 - -0.4 36,057 + +0.4 36,057 + +0.4 36,057 + -0.4 36,058 - -1.1 36,058 - +0.4 36,058 - -0.2 36,058 - +0.1 36,175 - -0.2 36,265 + -0.0 36,525 + GFF2956 0.12 -0.6 36,525 + GFF2956 0.12 +0.3 36,526 - GFF2956 0.12 -0.2 36,526 - GFF2956 0.12 +2.3 36,526 - GFF2956 0.12 +0.6 36,555 - GFF2956 0.12 +1.7 36,569 - GFF2956 0.13 +0.2 36,761 - GFF2956 0.17 -0.0 36,765 - GFF2956 0.17 +0.2 36,767 - GFF2956 0.17 -0.3 36,985 + GFF2956 0.22 -0.2 37,046 - GFF2956 0.23 +0.5 37,079 + GFF2956 0.24 +0.3 37,096 + GFF2956 0.25 +0.4 37,096 + GFF2956 0.25 +0.7 37,096 + GFF2956 0.25 -0.9 37,097 - GFF2956 0.25 -0.0 37,127 - GFF2956 0.25 +0.5 37,213 + GFF2956 0.27 -0.4 37,214 - GFF2956 0.27 +0.3 37,214 - GFF2956 0.27 +0.3 37,262 + GFF2956 0.28 +1.7 37,262 + GFF2956 0.28 -1.3 37,263 - GFF2956 0.28 +0.3 37,382 - GFF2956 0.31 -0.2 37,674 - GFF2956 0.38 +0.9 37,674 - GFF2956 0.38 -0.5 37,849 + GFF2956 0.41 +0.0 37,950 + GFF2956 0.44 -0.4 37,950 + GFF2956 0.44 -1.1 37,951 - GFF2956 0.44 +0.7 38,014 + GFF2956 0.45 +0.4 38,018 + GFF2956 0.45 -0.0 38,129 + GFF2956 0.48 +0.1 38,137 + GFF2956 0.48 +1.5 38,138 - GFF2956 0.48 -0.1 38,138 - GFF2956 0.48 -0.1 38,172 - GFF2956 0.49 -0.9 38,354 + GFF2956 0.53 -1.1 38,355 - GFF2956 0.53 -0.2 38,419 - GFF2956 0.54 -0.1 38,506 - GFF2956 0.56 +1.4 38,564 - GFF2956 0.57 -0.5 38,601 + GFF2956 0.58 +0.0 38,927 - GFF2956 0.66 +0.4 38,937 + GFF2956 0.66 +0.6 38,937 + GFF2956 0.66 -0.1 39,048 - GFF2956 0.68 +0.8 39,304 + GFF2956 0.74 +0.4 39,414 - GFF2956 0.77 -0.1 39,610 + GFF2956 0.81 -0.0 39,750 - GFF2956 0.84 +0.0 39,861 + GFF2956 0.87 +0.0 39,861 + GFF2956 0.87 +0.8 39,861 + GFF2956 0.87 -0.1 39,862 - GFF2956 0.87 -0.3 39,862 - GFF2956 0.87 +1.2 39,862 - GFF2956 0.87 +0.4 39,862 - GFF2956 0.87 +0.3 39,863 + GFF2956 0.87 -0.0 39,864 - GFF2956 0.87 +0.1 39,887 + GFF2956 0.87 -0.0 39,888 - GFF2956 0.87 +0.5 39,990 - GFF2956 0.89 +0.3 40,103 + +0.1 40,103 + -1.9 40,104 - +0.1 40,181 + -0.8 40,233 + +0.6 40,234 - +0.3 40,240 - +0.0 40,240 - +0.2 40,309 + -0.3 40,420 + -0.9 40,654 + GFF2957 0.13 -0.1 40,654 + GFF2957 0.13 +0.3 40,654 + GFF2957 0.13 +0.4 40,655 - GFF2957 0.13 +0.1 40,655 - GFF2957 0.13 -0.4 40,655 - GFF2957 0.13 +1.0 40,655 - GFF2957 0.13 -0.1 40,658 + GFF2957 0.13 +0.4 40,658 + GFF2957 0.13 +0.4 40,658 + GFF2957 0.13 +0.8 40,659 - GFF2957 0.13 +0.5 40,659 - GFF2957 0.13 -0.1 40,659 - GFF2957 0.13 +0.1 41,113 + GFF2957 0.45 -0.1 41,136 + GFF2957 0.47 -0.0 41,173 - GFF2957 0.50 -0.5 41,311 + GFF2957 0.59 -0.1 41,312 + GFF2957 0.59 +0.6 41,312 + GFF2957 0.59 -0.0 41,313 - GFF2957 0.59 +0.3 41,313 - GFF2957 0.59 -0.9 41,313 - GFF2957 0.59 -0.1 41,410 - GFF2957 0.66 -0.8 41,410 - GFF2957 0.66 -0.3
Or see this region's nucleotide sequence