Experiment: Acanthamoeba sp., muxed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2941 and GFF2942 are separated by 20 nucleotides GFF2942 and GFF2943 overlap by 4 nucleotides GFF2943 and GFF2944 overlap by 32 nucleotides GFF2944 and GFF2945 are separated by 6 nucleotides
GFF2941 - Arabinose 5-phosphate isomerase (EC 5.3.1.13), at 22,438 to 23,424
GFF2941
GFF2942 - 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase (EC 3.1.3.45), at 23,445 to 24,011
GFF2942
GFF2943 - Uncharacterized protein YrbK clustered with lipopolysaccharide transporters, at 24,008 to 24,583
GFF2943
GFF2944 - LptA, protein essential for LPS transport across the periplasm, at 24,552 to 25,106
GFF2944
GFF2945 - Lipopolysaccharide ABC transporter, ATP-binding protein LptB, at 25,113 to 25,838
GFF2945
Position (kb) 24
25 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 23.042 kb on + strand, within GFF2941 at 23.391 kb on + strand at 23.391 kb on + strand at 23.391 kb on + strand at 23.571 kb on + strand, within GFF2942 at 23.571 kb on + strand, within GFF2942 at 23.808 kb on + strand, within GFF2942 at 24.436 kb on + strand, within GFF2943 at 24.436 kb on + strand, within GFF2943 at 24.459 kb on + strand, within GFF2943
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2 remove 23,042 + GFF2941 0.61 +0.5 23,391 + -0.5 23,391 + +0.9 23,391 + +1.0 23,571 + GFF2942 0.22 -0.1 23,571 + GFF2942 0.22 -0.2 23,808 + GFF2942 0.64 -0.3 24,436 + GFF2943 0.74 -0.6 24,436 + GFF2943 0.74 +0.1 24,459 + GFF2943 0.78 -2.8
Or see this region's nucleotide sequence