Experiment: Acanthamoeba sp., muxed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2926 and GFF2927 are separated by 15 nucleotides GFF2927 and GFF2928 are separated by 5 nucleotides GFF2928 and GFF2929 are separated by 129 nucleotides GFF2929 and GFF2930 are separated by 19 nucleotides GFF2930 and GFF2931 are separated by 116 nucleotides
GFF2926 - GTP-binding protein Obg, at 11,695 to 12,867
GFF2926
GFF2927 - Permease of the drug/metabolite transporter (DMT) superfamily, at 12,883 to 13,242
GFF2927
GFF2928 - Permease of the drug/metabolite transporter (DMT) superfamily, at 13,248 to 13,847
GFF2928
GFF2929 - LSU ribosomal protein L27p, at 13,977 to 14,234
GFF2929
GFF2930 - LSU ribosomal protein L21p, at 14,254 to 14,565
GFF2930
GFF2931 - Octaprenyl diphosphate synthase (EC 2.5.1.90), at 14,682 to 15,794
GFF2931
Position (kb) 13
14 Strain fitness (log2 ratio) -2
-1
0
1 at 12.943 kb on - strand, within GFF2927 at 12.963 kb on - strand, within GFF2927 at 13.075 kb on - strand, within GFF2927 at 13.157 kb on - strand, within GFF2927 at 13.306 kb on - strand at 13.327 kb on + strand, within GFF2928 at 13.379 kb on - strand, within GFF2928 at 13.717 kb on - strand, within GFF2928 at 13.717 kb on - strand, within GFF2928 at 13.717 kb on - strand, within GFF2928 at 13.717 kb on - strand, within GFF2928 at 13.901 kb on - strand at 14.671 kb on - strand at 14.680 kb on - strand at 14.705 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2 remove 12,943 - GFF2927 0.17 +0.2 12,963 - GFF2927 0.22 +0.2 13,075 - GFF2927 0.53 +0.4 13,157 - GFF2927 0.76 +0.2 13,306 - +0.3 13,327 + GFF2928 0.13 -2.0 13,379 - GFF2928 0.22 -1.2 13,717 - GFF2928 0.78 -0.4 13,717 - GFF2928 0.78 -0.1 13,717 - GFF2928 0.78 -0.4 13,717 - GFF2928 0.78 -0.3 13,901 - +0.2 14,671 - -1.0 14,680 - -0.6 14,705 + +0.1
Or see this region's nucleotide sequence