Experiment: Acanthamoeba sp., muxed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2804 and GFF2805 are separated by 322 nucleotides GFF2805 and GFF2806 are separated by 46 nucleotides GFF2806 and GFF2807 are separated by 219 nucleotides
GFF2804 - D-amino acid dehydrogenase small subunit (EC 1.4.99.1), at 327,958 to 329,256
GFF2804
GFF2805 - FIG004684: SpoVR-like protein, at 329,579 to 331,111
GFF2805
GFF2806 - Transcriptional regulator for fatty acid degradation FadR, GntR family, at 331,158 to 331,877
GFF2806
GFF2807 - Na+/H+ antiporter NhaB, at 332,097 to 333,641
GFF2807
Position (kb) 329
330
331
332 Strain fitness (log2 ratio) -2
-1
0
1
2 at 328.593 kb on + strand, within GFF2804 at 328.818 kb on - strand, within GFF2804 at 328.844 kb on - strand, within GFF2804 at 328.915 kb on - strand, within GFF2804 at 328.915 kb on - strand, within GFF2804 at 328.915 kb on - strand, within GFF2804 at 328.917 kb on - strand, within GFF2804 at 328.954 kb on - strand, within GFF2804 at 329.227 kb on + strand at 329.227 kb on + strand at 329.331 kb on + strand at 329.361 kb on + strand at 329.361 kb on + strand at 329.361 kb on + strand at 329.362 kb on - strand at 329.363 kb on + strand at 329.364 kb on - strand at 329.427 kb on + strand at 329.523 kb on - strand at 329.661 kb on - strand at 329.831 kb on + strand, within GFF2805 at 329.832 kb on - strand, within GFF2805 at 329.832 kb on - strand, within GFF2805 at 329.833 kb on + strand, within GFF2805 at 329.834 kb on - strand, within GFF2805 at 329.834 kb on - strand, within GFF2805 at 330.044 kb on + strand, within GFF2805 at 330.137 kb on + strand, within GFF2805 at 330.201 kb on - strand, within GFF2805 at 330.470 kb on + strand, within GFF2805 at 330.500 kb on - strand, within GFF2805 at 330.500 kb on - strand, within GFF2805 at 330.523 kb on + strand, within GFF2805 at 330.563 kb on + strand, within GFF2805 at 330.564 kb on - strand, within GFF2805 at 330.565 kb on + strand, within GFF2805 at 330.650 kb on + strand, within GFF2805 at 330.802 kb on + strand, within GFF2805 at 330.803 kb on - strand, within GFF2805 at 330.803 kb on - strand, within GFF2805 at 330.806 kb on + strand, within GFF2805 at 330.876 kb on - strand, within GFF2805 at 331.069 kb on - strand at 331.282 kb on - strand, within GFF2806 at 331.291 kb on - strand, within GFF2806 at 331.343 kb on - strand, within GFF2806 at 331.363 kb on + strand, within GFF2806 at 331.547 kb on + strand, within GFF2806 at 331.557 kb on - strand, within GFF2806 at 331.833 kb on - strand at 331.834 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2 remove 328,593 + GFF2804 0.49 +0.1 328,818 - GFF2804 0.66 +0.1 328,844 - GFF2804 0.68 +0.2 328,915 - GFF2804 0.74 +0.1 328,915 - GFF2804 0.74 +0.2 328,915 - GFF2804 0.74 +0.0 328,917 - GFF2804 0.74 -0.0 328,954 - GFF2804 0.77 +0.2 329,227 + -0.4 329,227 + +0.8 329,331 + -0.3 329,361 + -0.3 329,361 + +1.4 329,361 + +1.5 329,362 - +0.1 329,363 + +0.1 329,364 - +0.2 329,427 + -0.2 329,523 - -0.3 329,661 - +0.2 329,831 + GFF2805 0.16 +0.1 329,832 - GFF2805 0.17 -0.7 329,832 - GFF2805 0.17 +0.3 329,833 + GFF2805 0.17 -0.1 329,834 - GFF2805 0.17 +2.5 329,834 - GFF2805 0.17 -0.9 330,044 + GFF2805 0.30 -0.2 330,137 + GFF2805 0.36 +0.4 330,201 - GFF2805 0.41 +1.3 330,470 + GFF2805 0.58 +0.1 330,500 - GFF2805 0.60 -0.1 330,500 - GFF2805 0.60 +0.7 330,523 + GFF2805 0.62 -0.2 330,563 + GFF2805 0.64 +0.1 330,564 - GFF2805 0.64 +0.4 330,565 + GFF2805 0.64 +0.1 330,650 + GFF2805 0.70 +0.4 330,802 + GFF2805 0.80 -0.6 330,803 - GFF2805 0.80 +1.1 330,803 - GFF2805 0.80 -0.1 330,806 + GFF2805 0.80 +0.5 330,876 - GFF2805 0.85 +0.4 331,069 - -0.3 331,282 - GFF2806 0.17 -0.1 331,291 - GFF2806 0.18 +0.2 331,343 - GFF2806 0.26 -1.4 331,363 + GFF2806 0.28 -2.1 331,547 + GFF2806 0.54 -0.1 331,557 - GFF2806 0.55 -0.3 331,833 - +1.0 331,834 + +0.8
Or see this region's nucleotide sequence