Experiment: Acanthamoeba sp., muxed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF256 and GFF257 are separated by 79 nucleotides GFF257 and GFF258 are separated by 315 nucleotides
GFF256 - Putative arylsulfatase regulatory protein, at 31,252 to 32,547
GFF256
GFF257 - Putative carboxymethylenebutenolidase (EC 3.1.1.45), at 32,627 to 33,382
GFF257
GFF258 - Uridine phosphorylase (EC 2.4.2.3), at 33,698 to 34,459
GFF258
Position (kb)
32
33
34 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 31.646 kb on + strand, within GFF256 at 31.646 kb on + strand, within GFF256 at 31.666 kb on - strand, within GFF256 at 31.741 kb on - strand, within GFF256 at 31.744 kb on - strand, within GFF256 at 31.744 kb on - strand, within GFF256 at 31.824 kb on + strand, within GFF256 at 31.845 kb on - strand, within GFF256 at 31.864 kb on - strand, within GFF256 at 31.875 kb on - strand, within GFF256 at 31.878 kb on + strand, within GFF256 at 32.002 kb on + strand, within GFF256 at 32.003 kb on - strand, within GFF256 at 32.064 kb on - strand, within GFF256 at 32.186 kb on + strand, within GFF256 at 32.190 kb on + strand, within GFF256 at 32.191 kb on - strand, within GFF256 at 32.273 kb on + strand, within GFF256 at 32.274 kb on - strand, within GFF256 at 32.334 kb on + strand, within GFF256 at 32.338 kb on + strand, within GFF256 at 32.354 kb on - strand, within GFF256 at 32.356 kb on - strand, within GFF256 at 32.383 kb on + strand, within GFF256 at 32.384 kb on - strand, within GFF256 at 32.384 kb on - strand, within GFF256 at 32.385 kb on + strand, within GFF256 at 32.385 kb on + strand, within GFF256 at 32.385 kb on + strand, within GFF256 at 32.386 kb on - strand, within GFF256 at 32.386 kb on - strand, within GFF256 at 32.386 kb on - strand, within GFF256 at 32.487 kb on + strand at 32.538 kb on + strand at 32.539 kb on - strand at 32.545 kb on + strand at 32.549 kb on + strand at 32.549 kb on + strand at 32.615 kb on - strand at 32.615 kb on - strand at 32.726 kb on - strand, within GFF257 at 32.749 kb on - strand, within GFF257 at 32.951 kb on + strand, within GFF257 at 32.951 kb on + strand, within GFF257 at 32.952 kb on - strand, within GFF257 at 32.973 kb on + strand, within GFF257 at 32.974 kb on - strand, within GFF257 at 32.974 kb on - strand, within GFF257 at 32.984 kb on - strand, within GFF257 at 32.984 kb on - strand, within GFF257 at 33.095 kb on + strand, within GFF257 at 33.128 kb on + strand, within GFF257 at 33.129 kb on - strand, within GFF257 at 33.130 kb on + strand, within GFF257 at 33.316 kb on + strand at 33.443 kb on - strand at 33.475 kb on + strand at 33.479 kb on + strand at 33.479 kb on + strand at 33.481 kb on + strand at 33.608 kb on + strand at 33.647 kb on - strand at 33.695 kb on + strand at 33.696 kb on - strand at 33.696 kb on - strand at 33.697 kb on + strand at 33.895 kb on + strand, within GFF258 at 33.958 kb on + strand, within GFF258 at 33.958 kb on + strand, within GFF258 at 33.958 kb on + strand, within GFF258 at 33.959 kb on - strand, within GFF258 at 34.211 kb on + strand, within GFF258 at 34.247 kb on - strand, within GFF258 at 34.247 kb on - strand, within GFF258 at 34.298 kb on - strand, within GFF258
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2 remove 31,646 + GFF256 0.30 +0.3 31,646 + GFF256 0.30 -0.4 31,666 - GFF256 0.32 +0.3 31,741 - GFF256 0.38 +0.1 31,744 - GFF256 0.38 -1.6 31,744 - GFF256 0.38 -0.1 31,824 + GFF256 0.44 +0.1 31,845 - GFF256 0.46 +1.2 31,864 - GFF256 0.47 +0.0 31,875 - GFF256 0.48 -0.1 31,878 + GFF256 0.48 -0.1 32,002 + GFF256 0.58 +0.3 32,003 - GFF256 0.58 +0.2 32,064 - GFF256 0.63 +0.0 32,186 + GFF256 0.72 +0.1 32,190 + GFF256 0.72 -0.7 32,191 - GFF256 0.72 +0.1 32,273 + GFF256 0.79 +0.5 32,274 - GFF256 0.79 +0.1 32,334 + GFF256 0.83 -0.2 32,338 + GFF256 0.84 +0.1 32,354 - GFF256 0.85 +0.1 32,356 - GFF256 0.85 -1.2 32,383 + GFF256 0.87 -2.0 32,384 - GFF256 0.87 -0.2 32,384 - GFF256 0.87 +1.9 32,385 + GFF256 0.87 -0.6 32,385 + GFF256 0.87 +2.0 32,385 + GFF256 0.87 +0.6 32,386 - GFF256 0.88 +0.1 32,386 - GFF256 0.88 -0.4 32,386 - GFF256 0.88 +0.5 32,487 + +0.1 32,538 + +0.6 32,539 - +0.1 32,545 + -0.0 32,549 + -0.2 32,549 + -1.6 32,615 - +0.6 32,615 - +0.1 32,726 - GFF257 0.13 -1.2 32,749 - GFF257 0.16 -0.6 32,951 + GFF257 0.43 +1.0 32,951 + GFF257 0.43 -0.3 32,952 - GFF257 0.43 +0.4 32,973 + GFF257 0.46 -0.0 32,974 - GFF257 0.46 -0.8 32,974 - GFF257 0.46 -0.6 32,984 - GFF257 0.47 +0.0 32,984 - GFF257 0.47 -0.6 33,095 + GFF257 0.62 +0.2 33,128 + GFF257 0.66 -0.3 33,129 - GFF257 0.66 +0.4 33,130 + GFF257 0.67 -0.3 33,316 + -0.1 33,443 - +0.0 33,475 + +0.2 33,479 + +0.0 33,479 + -0.2 33,481 + -0.3 33,608 + -0.4 33,647 - -0.0 33,695 + +0.3 33,696 - -0.9 33,696 - -0.2 33,697 + +0.7 33,895 + GFF258 0.26 -0.0 33,958 + GFF258 0.34 -0.0 33,958 + GFF258 0.34 +0.2 33,958 + GFF258 0.34 +0.2 33,959 - GFF258 0.34 -0.4 34,211 + GFF258 0.67 +0.7 34,247 - GFF258 0.72 -0.1 34,247 - GFF258 0.72 +0.4 34,298 - GFF258 0.79 -0.3
Or see this region's nucleotide sequence