Experiment: Acanthamoeba sp., muxed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2558 and GFF2559 overlap by 8 nucleotides GFF2559 and GFF2560 are separated by 13 nucleotides GFF2560 and GFF2561 are separated by 22 nucleotides GFF2561 and GFF2562 are separated by 159 nucleotides
GFF2558 - Formate dehydrogenase N gamma subunit (EC 1.2.1.2), at 74,512 to 75,168
GFF2558
GFF2559 - Formate dehydrogenase N beta subunit (EC 1.2.1.2), at 75,161 to 76,045
GFF2559
GFF2560 - Formate dehydrogenase N alpha subunit (EC 1.2.1.2) @ selenocysteine-containing, at 76,059 to 79,106
GFF2560
GFF2561 - hypothetical protein, at 79,129 to 79,281
GFF2561
GFF2562 - Permease of the drug/metabolite transporter (DMT) superfamily, at 79,441 to 80,265
GFF2562
Position (kb) 76
77
78
79
80 Strain fitness (log2 ratio) -2
-1
0
1 at 75.220 kb on - strand at 75.220 kb on - strand at 75.369 kb on + strand, within GFF2559 at 75.409 kb on + strand, within GFF2559 at 75.745 kb on + strand, within GFF2559 at 75.745 kb on + strand, within GFF2559 at 75.746 kb on - strand, within GFF2559 at 75.850 kb on - strand, within GFF2559 at 75.883 kb on + strand, within GFF2559 at 75.969 kb on + strand at 76.045 kb on + strand at 76.046 kb on - strand at 76.297 kb on + strand at 76.298 kb on - strand at 76.298 kb on - strand at 76.314 kb on - strand at 76.377 kb on - strand, within GFF2560 at 76.377 kb on - strand, within GFF2560 at 76.381 kb on - strand, within GFF2560 at 76.400 kb on + strand, within GFF2560 at 76.424 kb on - strand, within GFF2560 at 76.424 kb on - strand, within GFF2560 at 76.483 kb on + strand, within GFF2560 at 76.589 kb on + strand, within GFF2560 at 76.596 kb on - strand, within GFF2560 at 76.667 kb on + strand, within GFF2560 at 76.771 kb on + strand, within GFF2560 at 76.977 kb on - strand, within GFF2560 at 77.009 kb on - strand, within GFF2560 at 77.232 kb on - strand, within GFF2560 at 77.309 kb on + strand, within GFF2560 at 77.309 kb on + strand, within GFF2560 at 77.310 kb on - strand, within GFF2560 at 77.375 kb on - strand, within GFF2560 at 77.504 kb on - strand, within GFF2560 at 77.617 kb on + strand, within GFF2560 at 77.666 kb on - strand, within GFF2560 at 77.837 kb on + strand, within GFF2560 at 77.838 kb on - strand, within GFF2560 at 77.884 kb on + strand, within GFF2560 at 77.885 kb on - strand, within GFF2560 at 77.995 kb on + strand, within GFF2560 at 78.088 kb on + strand, within GFF2560 at 78.089 kb on - strand, within GFF2560 at 78.105 kb on - strand, within GFF2560 at 78.194 kb on - strand, within GFF2560 at 78.194 kb on - strand, within GFF2560 at 78.293 kb on - strand, within GFF2560 at 78.293 kb on - strand, within GFF2560 at 78.446 kb on + strand, within GFF2560 at 78.449 kb on + strand, within GFF2560 at 78.735 kb on - strand, within GFF2560 at 78.783 kb on + strand, within GFF2560 at 78.784 kb on - strand, within GFF2560 at 78.805 kb on + strand at 78.920 kb on + strand at 78.922 kb on + strand at 78.922 kb on + strand at 78.923 kb on - strand at 78.992 kb on + strand at 79.029 kb on - strand at 79.196 kb on + strand, within GFF2561 at 79.254 kb on - strand, within GFF2561 at 79.279 kb on + strand at 79.339 kb on + strand at 79.406 kb on + strand at 79.409 kb on + strand at 79.410 kb on - strand at 79.505 kb on - strand at 79.505 kb on - strand at 79.545 kb on - strand, within GFF2562 at 79.573 kb on + strand, within GFF2562 at 79.686 kb on - strand, within GFF2562 at 79.827 kb on + strand, within GFF2562 at 79.884 kb on + strand, within GFF2562 at 79.919 kb on - strand, within GFF2562 at 79.979 kb on + strand, within GFF2562 at 80.013 kb on - strand, within GFF2562 at 80.093 kb on + strand, within GFF2562
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2 remove 75,220 - +0.5 75,220 - +0.8 75,369 + GFF2559 0.24 -0.3 75,409 + GFF2559 0.28 -0.1 75,745 + GFF2559 0.66 +0.4 75,745 + GFF2559 0.66 -1.5 75,746 - GFF2559 0.66 -0.5 75,850 - GFF2559 0.78 +0.0 75,883 + GFF2559 0.82 -0.5 75,969 + -0.2 76,045 + -0.1 76,046 - -0.4 76,297 + +0.4 76,298 - -0.6 76,298 - +1.7 76,314 - +0.4 76,377 - GFF2560 0.10 -0.8 76,377 - GFF2560 0.10 +0.4 76,381 - GFF2560 0.11 -1.0 76,400 + GFF2560 0.11 +0.5 76,424 - GFF2560 0.12 +0.4 76,424 - GFF2560 0.12 -0.1 76,483 + GFF2560 0.14 -0.9 76,589 + GFF2560 0.17 -0.3 76,596 - GFF2560 0.18 -0.3 76,667 + GFF2560 0.20 -0.0 76,771 + GFF2560 0.23 +0.1 76,977 - GFF2560 0.30 -0.2 77,009 - GFF2560 0.31 -0.5 77,232 - GFF2560 0.38 +1.7 77,309 + GFF2560 0.41 -0.1 77,309 + GFF2560 0.41 +0.1 77,310 - GFF2560 0.41 +0.2 77,375 - GFF2560 0.43 +0.1 77,504 - GFF2560 0.47 -0.2 77,617 + GFF2560 0.51 +0.4 77,666 - GFF2560 0.53 -0.9 77,837 + GFF2560 0.58 -0.2 77,838 - GFF2560 0.58 +0.3 77,884 + GFF2560 0.60 +0.1 77,885 - GFF2560 0.60 +0.2 77,995 + GFF2560 0.64 -0.0 78,088 + GFF2560 0.67 -0.1 78,089 - GFF2560 0.67 +0.5 78,105 - GFF2560 0.67 -0.5 78,194 - GFF2560 0.70 -1.3 78,194 - GFF2560 0.70 -2.7 78,293 - GFF2560 0.73 +1.3 78,293 - GFF2560 0.73 -0.0 78,446 + GFF2560 0.78 -0.7 78,449 + GFF2560 0.78 -0.1 78,735 - GFF2560 0.88 -0.4 78,783 + GFF2560 0.89 +0.1 78,784 - GFF2560 0.89 -0.4 78,805 + +0.2 78,920 + +0.2 78,922 + +0.5 78,922 + +0.4 78,923 - +0.2 78,992 + +0.6 79,029 - +0.4 79,196 + GFF2561 0.44 +1.1 79,254 - GFF2561 0.82 -0.0 79,279 + -0.6 79,339 + -1.1 79,406 + -0.5 79,409 + -0.4 79,410 - +0.1 79,505 - -0.3 79,505 - +0.7 79,545 - GFF2562 0.13 +0.3 79,573 + GFF2562 0.16 +1.3 79,686 - GFF2562 0.30 +0.1 79,827 + GFF2562 0.47 -0.3 79,884 + GFF2562 0.54 -0.7 79,919 - GFF2562 0.58 -0.2 79,979 + GFF2562 0.65 +0.8 80,013 - GFF2562 0.69 +0.2 80,093 + GFF2562 0.79 +1.4
Or see this region's nucleotide sequence