Experiment: Acanthamoeba sp., muxed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2557 and GFF2558 are separated by 90 nucleotides GFF2558 and GFF2559 overlap by 8 nucleotides GFF2559 and GFF2560 are separated by 13 nucleotides
GFF2557 - Alcohol dehydrogenase (EC 1.1.1.1), at 73,411 to 74,421
GFF2557
GFF2558 - Formate dehydrogenase N gamma subunit (EC 1.2.1.2), at 74,512 to 75,168
GFF2558
GFF2559 - Formate dehydrogenase N beta subunit (EC 1.2.1.2), at 75,161 to 76,045
GFF2559
GFF2560 - Formate dehydrogenase N alpha subunit (EC 1.2.1.2) @ selenocysteine-containing, at 76,059 to 79,106
GFF2560
Position (kb)
75
76
77 Strain fitness (log2 ratio)
-1
0
1 at 74.398 kb on - strand at 74.445 kb on + strand at 74.658 kb on - strand, within GFF2558 at 74.658 kb on - strand, within GFF2558 at 74.658 kb on - strand, within GFF2558 at 74.661 kb on + strand, within GFF2558 at 74.662 kb on - strand, within GFF2558 at 74.662 kb on - strand, within GFF2558 at 74.662 kb on - strand, within GFF2558 at 74.718 kb on + strand, within GFF2558 at 74.719 kb on - strand, within GFF2558 at 74.808 kb on + strand, within GFF2558 at 74.809 kb on - strand, within GFF2558 at 74.844 kb on - strand, within GFF2558 at 74.844 kb on - strand, within GFF2558 at 74.865 kb on + strand, within GFF2558 at 74.866 kb on - strand, within GFF2558 at 74.885 kb on - strand, within GFF2558 at 75.050 kb on - strand, within GFF2558 at 75.220 kb on - strand at 75.220 kb on - strand at 75.369 kb on + strand, within GFF2559 at 75.409 kb on + strand, within GFF2559 at 75.745 kb on + strand, within GFF2559 at 75.745 kb on + strand, within GFF2559 at 75.746 kb on - strand, within GFF2559 at 75.850 kb on - strand, within GFF2559 at 75.883 kb on + strand, within GFF2559 at 75.969 kb on + strand at 76.045 kb on + strand at 76.046 kb on - strand at 76.297 kb on + strand at 76.298 kb on - strand at 76.298 kb on - strand at 76.314 kb on - strand at 76.377 kb on - strand, within GFF2560 at 76.377 kb on - strand, within GFF2560 at 76.381 kb on - strand, within GFF2560 at 76.400 kb on + strand, within GFF2560 at 76.424 kb on - strand, within GFF2560 at 76.424 kb on - strand, within GFF2560 at 76.483 kb on + strand, within GFF2560 at 76.589 kb on + strand, within GFF2560 at 76.596 kb on - strand, within GFF2560 at 76.667 kb on + strand, within GFF2560 at 76.771 kb on + strand, within GFF2560 at 76.977 kb on - strand, within GFF2560 at 77.009 kb on - strand, within GFF2560
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2 remove 74,398 - +0.2 74,445 + +0.4 74,658 - GFF2558 0.22 +0.2 74,658 - GFF2558 0.22 +0.3 74,658 - GFF2558 0.22 +0.4 74,661 + GFF2558 0.23 -1.2 74,662 - GFF2558 0.23 +1.1 74,662 - GFF2558 0.23 -0.2 74,662 - GFF2558 0.23 -0.3 74,718 + GFF2558 0.31 +0.1 74,719 - GFF2558 0.32 +0.1 74,808 + GFF2558 0.45 +0.3 74,809 - GFF2558 0.45 -0.4 74,844 - GFF2558 0.51 -0.0 74,844 - GFF2558 0.51 +0.3 74,865 + GFF2558 0.54 +0.3 74,866 - GFF2558 0.54 +0.3 74,885 - GFF2558 0.57 -0.8 75,050 - GFF2558 0.82 -0.1 75,220 - +0.5 75,220 - +0.8 75,369 + GFF2559 0.24 -0.3 75,409 + GFF2559 0.28 -0.1 75,745 + GFF2559 0.66 +0.4 75,745 + GFF2559 0.66 -1.5 75,746 - GFF2559 0.66 -0.5 75,850 - GFF2559 0.78 +0.0 75,883 + GFF2559 0.82 -0.5 75,969 + -0.2 76,045 + -0.1 76,046 - -0.4 76,297 + +0.4 76,298 - -0.6 76,298 - +1.7 76,314 - +0.4 76,377 - GFF2560 0.10 -0.8 76,377 - GFF2560 0.10 +0.4 76,381 - GFF2560 0.11 -1.0 76,400 + GFF2560 0.11 +0.5 76,424 - GFF2560 0.12 +0.4 76,424 - GFF2560 0.12 -0.1 76,483 + GFF2560 0.14 -0.9 76,589 + GFF2560 0.17 -0.3 76,596 - GFF2560 0.18 -0.3 76,667 + GFF2560 0.20 -0.0 76,771 + GFF2560 0.23 +0.1 76,977 - GFF2560 0.30 -0.2 77,009 - GFF2560 0.31 -0.5
Or see this region's nucleotide sequence