Experiment: Acanthamoeba sp., muxed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF245 and GFF246 are separated by 43 nucleotides GFF246 and GFF247 are separated by 164 nucleotides GFF247 and GFF248 are separated by 65 nucleotides
GFF245 - Protein rarD, at 18,897 to 19,781
GFF245
GFF246 - putative protein PaaI, possibly involved in aromatic compounds catabolism, at 19,825 to 20,292
GFF246
GFF247 - Phospholipase A1 precursor (EC 3.1.1.32, EC 3.1.1.4); Outer membrane phospholipase A, at 20,457 to 21,326
GFF247
GFF248 - ATP-dependent DNA helicase RecQ, at 21,392 to 23,239
GFF248
Position (kb)
20
21
22 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 19.466 kb on - strand, within GFF245 at 19.487 kb on + strand, within GFF245 at 19.488 kb on - strand, within GFF245 at 19.488 kb on - strand, within GFF245 at 19.488 kb on - strand, within GFF245 at 19.691 kb on - strand, within GFF245 at 19.805 kb on + strand at 19.806 kb on - strand at 19.836 kb on + strand at 19.836 kb on + strand at 19.838 kb on + strand at 19.838 kb on + strand at 19.838 kb on + strand at 19.839 kb on - strand at 19.862 kb on + strand at 19.862 kb on + strand at 19.883 kb on - strand, within GFF246 at 20.017 kb on + strand, within GFF246 at 20.117 kb on - strand, within GFF246 at 20.382 kb on - strand at 20.397 kb on + strand at 20.433 kb on + strand at 20.511 kb on + strand at 20.511 kb on + strand at 20.525 kb on + strand at 20.557 kb on + strand, within GFF247 at 20.584 kb on + strand, within GFF247 at 20.584 kb on + strand, within GFF247 at 20.585 kb on - strand, within GFF247 at 20.597 kb on + strand, within GFF247 at 20.606 kb on + strand, within GFF247 at 20.614 kb on - strand, within GFF247 at 20.619 kb on + strand, within GFF247 at 20.631 kb on + strand, within GFF247 at 20.632 kb on - strand, within GFF247 at 20.641 kb on - strand, within GFF247 at 20.674 kb on + strand, within GFF247 at 20.675 kb on - strand, within GFF247 at 20.678 kb on + strand, within GFF247 at 20.679 kb on - strand, within GFF247 at 20.705 kb on + strand, within GFF247 at 20.759 kb on - strand, within GFF247 at 20.791 kb on - strand, within GFF247 at 20.791 kb on - strand, within GFF247 at 20.793 kb on - strand, within GFF247 at 20.793 kb on - strand, within GFF247 at 20.826 kb on - strand, within GFF247 at 20.826 kb on - strand, within GFF247 at 20.826 kb on - strand, within GFF247 at 20.857 kb on - strand, within GFF247 at 20.924 kb on - strand, within GFF247 at 20.935 kb on - strand, within GFF247 at 20.991 kb on + strand, within GFF247 at 20.991 kb on + strand, within GFF247 at 20.991 kb on + strand, within GFF247 at 20.992 kb on - strand, within GFF247 at 21.028 kb on + strand, within GFF247 at 21.046 kb on - strand, within GFF247 at 21.087 kb on + strand, within GFF247 at 21.090 kb on - strand, within GFF247 at 21.157 kb on - strand, within GFF247 at 21.196 kb on + strand, within GFF247 at 21.197 kb on - strand, within GFF247 at 21.228 kb on + strand, within GFF247 at 21.229 kb on - strand, within GFF247 at 21.229 kb on - strand, within GFF247 at 21.318 kb on - strand at 21.318 kb on - strand at 21.318 kb on - strand at 21.406 kb on + strand at 21.509 kb on - strand at 21.579 kb on - strand, within GFF248 at 21.768 kb on - strand, within GFF248 at 21.770 kb on - strand, within GFF248 at 21.983 kb on - strand, within GFF248 at 21.986 kb on - strand, within GFF248 at 22.024 kb on + strand, within GFF248 at 22.052 kb on + strand, within GFF248 at 22.097 kb on + strand, within GFF248 at 22.133 kb on + strand, within GFF248 at 22.134 kb on - strand, within GFF248 at 22.309 kb on - strand, within GFF248 at 22.318 kb on + strand, within GFF248 at 22.319 kb on - strand, within GFF248
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2 remove 19,466 - GFF245 0.64 +0.3 19,487 + GFF245 0.67 +0.6 19,488 - GFF245 0.67 -0.3 19,488 - GFF245 0.67 -0.1 19,488 - GFF245 0.67 -0.4 19,691 - GFF245 0.90 -0.4 19,805 + +0.0 19,806 - -0.4 19,836 + +0.2 19,836 + -0.2 19,838 + +2.1 19,838 + -0.6 19,838 + +0.7 19,839 - -0.8 19,862 + -1.9 19,862 + +0.0 19,883 - GFF246 0.12 +0.8 20,017 + GFF246 0.41 -0.0 20,117 - GFF246 0.62 +0.3 20,382 - +0.4 20,397 + -0.1 20,433 + -0.5 20,511 + +0.1 20,511 + -0.6 20,525 + +0.3 20,557 + GFF247 0.11 +0.2 20,584 + GFF247 0.15 -0.0 20,584 + GFF247 0.15 +0.7 20,585 - GFF247 0.15 -0.3 20,597 + GFF247 0.16 -0.4 20,606 + GFF247 0.17 +2.3 20,614 - GFF247 0.18 +0.0 20,619 + GFF247 0.19 +0.7 20,631 + GFF247 0.20 -0.1 20,632 - GFF247 0.20 +0.1 20,641 - GFF247 0.21 +0.7 20,674 + GFF247 0.25 +0.4 20,675 - GFF247 0.25 +0.7 20,678 + GFF247 0.25 +0.2 20,679 - GFF247 0.26 +0.1 20,705 + GFF247 0.29 +0.4 20,759 - GFF247 0.35 +0.0 20,791 - GFF247 0.38 -0.3 20,791 - GFF247 0.38 -0.4 20,793 - GFF247 0.39 +1.0 20,793 - GFF247 0.39 +0.4 20,826 - GFF247 0.42 -0.4 20,826 - GFF247 0.42 -0.7 20,826 - GFF247 0.42 +0.1 20,857 - GFF247 0.46 +0.3 20,924 - GFF247 0.54 -0.4 20,935 - GFF247 0.55 +0.2 20,991 + GFF247 0.61 -0.1 20,991 + GFF247 0.61 -0.2 20,991 + GFF247 0.61 -0.1 20,992 - GFF247 0.61 +0.1 21,028 + GFF247 0.66 +0.1 21,046 - GFF247 0.68 -0.4 21,087 + GFF247 0.72 -0.1 21,090 - GFF247 0.73 +0.3 21,157 - GFF247 0.80 -0.5 21,196 + GFF247 0.85 -0.0 21,197 - GFF247 0.85 +0.6 21,228 + GFF247 0.89 -0.1 21,229 - GFF247 0.89 -0.4 21,229 - GFF247 0.89 +0.4 21,318 - +0.3 21,318 - -0.1 21,318 - -0.1 21,406 + -0.1 21,509 - -0.3 21,579 - GFF248 0.10 +0.4 21,768 - GFF248 0.20 -0.1 21,770 - GFF248 0.20 +0.2 21,983 - GFF248 0.32 +0.1 21,986 - GFF248 0.32 -1.4 22,024 + GFF248 0.34 -0.2 22,052 + GFF248 0.36 -0.7 22,097 + GFF248 0.38 +0.4 22,133 + GFF248 0.40 -0.4 22,134 - GFF248 0.40 -0.0 22,309 - GFF248 0.50 +0.0 22,318 + GFF248 0.50 +0.2 22,319 - GFF248 0.50 -0.3
Or see this region's nucleotide sequence