Experiment: Acanthamoeba sp., muxed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2417 and GFF2418 overlap by 8 nucleotides GFF2418 and GFF2419 overlap by 4 nucleotides GFF2419 and GFF2420 are separated by 155 nucleotides
GFF2417 - Anaerobic dimethyl sulfoxide reductase chain C (EC 1.8.5.3), at 29,969 to 30,778
GFF2417
GFF2418 - Anaerobic dimethyl sulfoxide reductase chain B (EC 1.8.5.3), at 30,771 to 31,400
GFF2418
GFF2419 - Anaerobic dimethyl sulfoxide reductase chain A (EC 1.8.99.-), at 31,397 to 33,775
GFF2419
GFF2420 - Penicillin-insensitive transglycosylase (EC 2.4.2.-) & transpeptidase PBP-1C, at 33,931 to 36,246
GFF2420
Position (kb)
31
32
33
34 Strain fitness (log2 ratio)
-1
0
1 at 30.410 kb on + strand, within GFF2417 at 30.510 kb on + strand, within GFF2417 at 30.563 kb on + strand, within GFF2417 at 30.563 kb on + strand, within GFF2417 at 30.563 kb on + strand, within GFF2417 at 30.693 kb on + strand, within GFF2417 at 30.768 kb on - strand at 30.992 kb on + strand, within GFF2418 at 31.055 kb on - strand, within GFF2418 at 31.405 kb on + strand at 31.406 kb on - strand at 31.406 kb on - strand at 31.607 kb on - strand at 31.633 kb on + strand at 31.918 kb on + strand, within GFF2419 at 31.937 kb on - strand, within GFF2419 at 32.091 kb on - strand, within GFF2419 at 32.121 kb on + strand, within GFF2419 at 32.176 kb on - strand, within GFF2419 at 32.219 kb on - strand, within GFF2419 at 32.240 kb on + strand, within GFF2419 at 32.241 kb on - strand, within GFF2419 at 32.241 kb on - strand, within GFF2419 at 32.250 kb on + strand, within GFF2419 at 32.251 kb on - strand, within GFF2419 at 32.318 kb on + strand, within GFF2419 at 32.362 kb on - strand, within GFF2419 at 32.395 kb on + strand, within GFF2419 at 32.395 kb on + strand, within GFF2419 at 32.606 kb on + strand, within GFF2419 at 32.860 kb on - strand, within GFF2419 at 33.048 kb on + strand, within GFF2419 at 33.056 kb on - strand, within GFF2419 at 33.157 kb on + strand, within GFF2419 at 33.158 kb on - strand, within GFF2419 at 33.158 kb on - strand, within GFF2419 at 33.158 kb on - strand, within GFF2419 at 33.298 kb on - strand, within GFF2419 at 33.427 kb on + strand, within GFF2419 at 33.427 kb on + strand, within GFF2419 at 34.365 kb on + strand, within GFF2420 at 34.563 kb on + strand, within GFF2420 at 34.656 kb on + strand, within GFF2420 at 34.659 kb on - strand, within GFF2420 at 34.700 kb on + strand, within GFF2420
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2 remove 30,410 + GFF2417 0.54 +0.2 30,510 + GFF2417 0.67 +0.1 30,563 + GFF2417 0.73 +0.6 30,563 + GFF2417 0.73 +0.2 30,563 + GFF2417 0.73 +0.1 30,693 + GFF2417 0.89 +1.0 30,768 - +0.4 30,992 + GFF2418 0.35 +0.3 31,055 - GFF2418 0.45 +0.4 31,405 + +0.1 31,406 - -1.0 31,406 - +0.3 31,607 - -0.3 31,633 + -0.2 31,918 + GFF2419 0.22 -0.6 31,937 - GFF2419 0.23 -0.2 32,091 - GFF2419 0.29 +0.6 32,121 + GFF2419 0.30 -0.9 32,176 - GFF2419 0.33 +0.2 32,219 - GFF2419 0.35 -0.5 32,240 + GFF2419 0.35 -1.1 32,241 - GFF2419 0.35 -0.2 32,241 - GFF2419 0.35 +0.1 32,250 + GFF2419 0.36 +0.2 32,251 - GFF2419 0.36 -0.3 32,318 + GFF2419 0.39 +0.6 32,362 - GFF2419 0.41 +0.5 32,395 + GFF2419 0.42 +1.0 32,395 + GFF2419 0.42 +0.5 32,606 + GFF2419 0.51 +0.1 32,860 - GFF2419 0.61 +0.3 33,048 + GFF2419 0.69 +0.4 33,056 - GFF2419 0.70 +0.1 33,157 + GFF2419 0.74 +0.5 33,158 - GFF2419 0.74 +0.5 33,158 - GFF2419 0.74 -0.3 33,158 - GFF2419 0.74 +0.2 33,298 - GFF2419 0.80 -0.1 33,427 + GFF2419 0.85 +0.0 33,427 + GFF2419 0.85 +0.2 34,365 + GFF2420 0.19 +0.3 34,563 + GFF2420 0.27 +0.3 34,656 + GFF2420 0.31 +1.7 34,659 - GFF2420 0.31 -0.7 34,700 + GFF2420 0.33 -0.2
Or see this region's nucleotide sequence