Experiment: Acanthamoeba sp., muxed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2220 and GFF2221 are separated by 8 nucleotides GFF2221 and GFF2222 are separated by 274 nucleotides
GFF2220 - Nitrogen regulation protein NR(I), at 11,781 to 13,190
GFF2220
GFF2221 - Nitrogen regulation protein NtrB (EC 2.7.13.3), at 13,199 to 14,248
GFF2221
GFF2222 - Glutamine synthetase type I (EC 6.3.1.2), at 14,523 to 15,932
GFF2222
Position (kb) 13
14
15 Strain fitness (log2 ratio) -2
-1
0
1 at 12.406 kb on + strand, within GFF2220 at 12.406 kb on + strand, within GFF2220 at 12.407 kb on - strand, within GFF2220 at 12.639 kb on - strand, within GFF2220 at 12.704 kb on + strand, within GFF2220 at 12.731 kb on + strand, within GFF2220 at 12.731 kb on + strand, within GFF2220 at 12.769 kb on - strand, within GFF2220 at 12.804 kb on - strand, within GFF2220 at 12.804 kb on - strand, within GFF2220 at 12.826 kb on + strand, within GFF2220 at 12.869 kb on + strand, within GFF2220 at 12.869 kb on + strand, within GFF2220 at 12.870 kb on - strand, within GFF2220 at 12.930 kb on + strand, within GFF2220 at 12.950 kb on + strand, within GFF2220 at 12.950 kb on + strand, within GFF2220 at 12.983 kb on + strand, within GFF2220 at 12.984 kb on - strand, within GFF2220 at 13.101 kb on - strand at 13.154 kb on + strand at 13.154 kb on + strand at 13.154 kb on + strand at 13.154 kb on + strand at 13.155 kb on - strand at 13.155 kb on - strand at 13.155 kb on - strand at 13.191 kb on - strand at 13.191 kb on - strand at 13.191 kb on - strand at 13.298 kb on - strand at 13.298 kb on - strand at 13.301 kb on + strand at 13.403 kb on - strand, within GFF2221 at 13.516 kb on + strand, within GFF2221 at 13.516 kb on + strand, within GFF2221 at 13.516 kb on + strand, within GFF2221 at 13.516 kb on + strand, within GFF2221 at 13.517 kb on - strand, within GFF2221 at 13.526 kb on + strand, within GFF2221 at 13.579 kb on - strand, within GFF2221 at 13.649 kb on - strand, within GFF2221 at 13.652 kb on + strand, within GFF2221 at 13.652 kb on + strand, within GFF2221 at 13.652 kb on + strand, within GFF2221 at 13.653 kb on - strand, within GFF2221 at 13.715 kb on + strand, within GFF2221 at 13.716 kb on - strand, within GFF2221 at 13.750 kb on - strand, within GFF2221 at 13.787 kb on + strand, within GFF2221 at 13.937 kb on + strand, within GFF2221 at 13.938 kb on - strand, within GFF2221 at 13.939 kb on + strand, within GFF2221 at 13.939 kb on + strand, within GFF2221 at 14.300 kb on - strand at 14.453 kb on + strand at 14.453 kb on + strand at 14.454 kb on - strand at 14.454 kb on - strand at 14.454 kb on - strand at 14.454 kb on - strand at 14.454 kb on - strand at 14.533 kb on + strand at 14.567 kb on + strand at 14.568 kb on - strand at 14.605 kb on + strand at 14.605 kb on + strand at 15.041 kb on - strand, within GFF2222 at 15.044 kb on - strand, within GFF2222 at 15.044 kb on - strand, within GFF2222 at 15.212 kb on - strand, within GFF2222 at 15.212 kb on - strand, within GFF2222
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2 remove 12,406 + GFF2220 0.44 -2.7 12,406 + GFF2220 0.44 -0.6 12,407 - GFF2220 0.44 +1.3 12,639 - GFF2220 0.61 -0.3 12,704 + GFF2220 0.65 +1.3 12,731 + GFF2220 0.67 -0.3 12,731 + GFF2220 0.67 +1.4 12,769 - GFF2220 0.70 +0.2 12,804 - GFF2220 0.73 -0.1 12,804 - GFF2220 0.73 -0.1 12,826 + GFF2220 0.74 +0.0 12,869 + GFF2220 0.77 -0.1 12,869 + GFF2220 0.77 +0.3 12,870 - GFF2220 0.77 -1.0 12,930 + GFF2220 0.81 +0.3 12,950 + GFF2220 0.83 +0.1 12,950 + GFF2220 0.83 -0.8 12,983 + GFF2220 0.85 +0.3 12,984 - GFF2220 0.85 +1.0 13,101 - +0.1 13,154 + +0.7 13,154 + -0.8 13,154 + +0.2 13,154 + -0.6 13,155 - +0.2 13,155 - +0.4 13,155 - -0.3 13,191 - -0.3 13,191 - +0.3 13,191 - +0.4 13,298 - +1.1 13,298 - +0.8 13,301 + +0.5 13,403 - GFF2221 0.19 -0.3 13,516 + GFF2221 0.30 +0.2 13,516 + GFF2221 0.30 -0.5 13,516 + GFF2221 0.30 +0.3 13,516 + GFF2221 0.30 +0.3 13,517 - GFF2221 0.30 +0.3 13,526 + GFF2221 0.31 +0.3 13,579 - GFF2221 0.36 +1.2 13,649 - GFF2221 0.43 +1.0 13,652 + GFF2221 0.43 +0.7 13,652 + GFF2221 0.43 -1.0 13,652 + GFF2221 0.43 +0.9 13,653 - GFF2221 0.43 +0.4 13,715 + GFF2221 0.49 -0.9 13,716 - GFF2221 0.49 +0.4 13,750 - GFF2221 0.52 +1.1 13,787 + GFF2221 0.56 -1.5 13,937 + GFF2221 0.70 -0.1 13,938 - GFF2221 0.70 +0.8 13,939 + GFF2221 0.70 +0.2 13,939 + GFF2221 0.70 -0.6 14,300 - +0.0 14,453 + +0.1 14,453 + -0.7 14,454 - -1.3 14,454 - +0.5 14,454 - +0.7 14,454 - +0.3 14,454 - -1.1 14,533 + +0.2 14,567 + +0.7 14,568 - +0.7 14,605 + +0.3 14,605 + +0.7 15,041 - GFF2222 0.37 +0.9 15,044 - GFF2222 0.37 -1.1 15,044 - GFF2222 0.37 +0.2 15,212 - GFF2222 0.49 +1.0 15,212 - GFF2222 0.49 -1.3
Or see this region's nucleotide sequence