Experiment: Acanthamoeba sp., muxed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2118 and GFF2119 are separated by 72 nucleotides GFF2119 and GFF2120 are separated by 2 nucleotides GFF2120 and GFF2121 are separated by 150 nucleotides GFF2121 and GFF2122 are separated by 82 nucleotides
GFF2118 - FIG01046338: hypothetical protein, at 5,998 to 6,156
GFF2118
GFF2119 - Transcription repressor of multidrug efflux pump acrAB operon, TetR (AcrR) family, at 6,229 to 6,891
GFF2119
GFF2120 - FIG00554530: hypothetical protein, at 6,894 to 8,993
GFF2120
GFF2121 - FIG00638667: hypothetical protein, at 9,144 to 9,308
GFF2121
GFF2122 - Adenine-specific methyltransferase (EC 2.1.1.72), at 9,391 to 10,275
GFF2122
Position (kb)
6
7
8
9 Strain fitness (log2 ratio)
-2
-1
0
1 at 6.405 kb on + strand, within GFF2119 at 6.896 kb on - strand at 6.909 kb on - strand at 7.054 kb on - strand at 7.080 kb on + strand at 7.198 kb on + strand, within GFF2120 at 7.213 kb on + strand, within GFF2120 at 7.434 kb on + strand, within GFF2120 at 7.435 kb on - strand, within GFF2120 at 7.603 kb on - strand, within GFF2120 at 7.607 kb on + strand, within GFF2120 at 7.608 kb on - strand, within GFF2120 at 7.771 kb on + strand, within GFF2120 at 7.771 kb on + strand, within GFF2120 at 7.881 kb on - strand, within GFF2120 at 7.881 kb on - strand, within GFF2120 at 7.894 kb on - strand, within GFF2120 at 8.066 kb on - strand, within GFF2120 at 8.202 kb on - strand, within GFF2120 at 8.267 kb on + strand, within GFF2120 at 8.293 kb on - strand, within GFF2120 at 8.312 kb on + strand, within GFF2120 at 8.322 kb on - strand, within GFF2120 at 8.442 kb on - strand, within GFF2120 at 8.445 kb on + strand, within GFF2120 at 8.552 kb on + strand, within GFF2120 at 8.626 kb on + strand, within GFF2120 at 8.626 kb on + strand, within GFF2120 at 8.626 kb on + strand, within GFF2120 at 8.868 kb on + strand at 8.869 kb on - strand at 8.916 kb on + strand at 8.916 kb on + strand at 8.927 kb on - strand at 9.057 kb on - strand at 9.117 kb on + strand at 9.299 kb on + strand at 9.303 kb on + strand at 9.303 kb on + strand at 9.303 kb on + strand at 9.304 kb on - strand at 9.348 kb on + strand at 9.349 kb on - strand at 9.350 kb on + strand at 9.653 kb on - strand, within GFF2122 at 9.702 kb on - strand, within GFF2122 at 9.716 kb on + strand, within GFF2122 at 9.717 kb on - strand, within GFF2122 at 9.767 kb on + strand, within GFF2122 at 9.897 kb on - strand, within GFF2122 at 9.900 kb on + strand, within GFF2122 at 9.900 kb on + strand, within GFF2122 at 9.936 kb on + strand, within GFF2122 at 9.937 kb on - strand, within GFF2122 at 9.937 kb on - strand, within GFF2122 at 9.941 kb on + strand, within GFF2122 at 9.941 kb on + strand, within GFF2122
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2 remove 6,405 + GFF2119 0.27 +0.1 6,896 - -0.5 6,909 - -0.4 7,054 - +0.2 7,080 + -0.0 7,198 + GFF2120 0.14 -0.3 7,213 + GFF2120 0.15 -1.5 7,434 + GFF2120 0.26 -0.2 7,435 - GFF2120 0.26 -0.1 7,603 - GFF2120 0.34 -0.8 7,607 + GFF2120 0.34 +0.3 7,608 - GFF2120 0.34 +0.4 7,771 + GFF2120 0.42 -0.0 7,771 + GFF2120 0.42 -0.0 7,881 - GFF2120 0.47 +0.1 7,881 - GFF2120 0.47 +0.6 7,894 - GFF2120 0.48 +1.7 8,066 - GFF2120 0.56 -0.2 8,202 - GFF2120 0.62 -0.7 8,267 + GFF2120 0.65 +0.0 8,293 - GFF2120 0.67 -1.0 8,312 + GFF2120 0.68 +0.1 8,322 - GFF2120 0.68 -0.3 8,442 - GFF2120 0.74 -0.7 8,445 + GFF2120 0.74 -2.0 8,552 + GFF2120 0.79 +0.0 8,626 + GFF2120 0.82 -0.3 8,626 + GFF2120 0.82 +0.4 8,626 + GFF2120 0.82 +1.4 8,868 + -0.6 8,869 - +1.1 8,916 + -0.9 8,916 + -0.4 8,927 - +0.0 9,057 - +0.3 9,117 + -1.7 9,299 + +0.3 9,303 + -0.3 9,303 + -0.1 9,303 + +0.5 9,304 - -0.9 9,348 + +0.3 9,349 - +0.0 9,350 + -0.5 9,653 - GFF2122 0.30 -1.1 9,702 - GFF2122 0.35 +0.4 9,716 + GFF2122 0.37 +0.3 9,717 - GFF2122 0.37 -0.8 9,767 + GFF2122 0.42 -0.2 9,897 - GFF2122 0.57 +0.3 9,900 + GFF2122 0.58 -0.3 9,900 + GFF2122 0.58 -0.0 9,936 + GFF2122 0.62 +0.6 9,937 - GFF2122 0.62 -0.7 9,937 - GFF2122 0.62 +0.2 9,941 + GFF2122 0.62 +0.6 9,941 + GFF2122 0.62 -0.1
Or see this region's nucleotide sequence