Experiment: Acanthamoeba sp., muxed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1954 and GFF1955 overlap by 1 nucleotides GFF1955 and GFF1956 are separated by 177 nucleotides GFF1956 and GFF1957 are separated by 370 nucleotides
GFF1954 - Enoyl-CoA hydratase (EC 4.2.1.17) / Delta(3)-cis-delta(2)-trans-enoyl-CoA isomerase (EC 5.3.3.8) / 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35) / 3-hydroxybutyryl-CoA epimerase (EC 5.1.2.3), at 144,495 to 146,642
GFF1954
GFF1955 - 3-ketoacyl-CoA thiolase (EC 2.3.1.16) @ Acetyl-CoA acetyltransferase (EC 2.3.1.9), at 146,642 to 147,952
GFF1955
GFF1956 - FIG00637865: hypothetical protein, at 148,130 to 148,414
GFF1956
GFF1957 - Long-chain fatty acid transport protein, at 148,785 to 150,092
GFF1957
Position (kb)
146
147
148 Strain fitness (log2 ratio)
-1
0
1 at 145.917 kb on - strand, within GFF1954 at 145.959 kb on + strand, within GFF1954 at 145.991 kb on + strand, within GFF1954 at 146.246 kb on + strand, within GFF1954 at 146.247 kb on - strand, within GFF1954 at 146.421 kb on + strand, within GFF1954 at 146.433 kb on - strand at 146.454 kb on - strand at 146.785 kb on - strand, within GFF1955 at 146.891 kb on + strand, within GFF1955 at 147.006 kb on - strand, within GFF1955 at 147.006 kb on - strand, within GFF1955 at 147.055 kb on - strand, within GFF1955 at 147.265 kb on - strand, within GFF1955 at 147.265 kb on - strand, within GFF1955 at 147.365 kb on - strand, within GFF1955 at 147.365 kb on - strand, within GFF1955 at 147.369 kb on + strand, within GFF1955 at 147.370 kb on - strand, within GFF1955 at 147.669 kb on - strand, within GFF1955 at 147.728 kb on - strand, within GFF1955 at 147.750 kb on - strand, within GFF1955 at 147.752 kb on + strand, within GFF1955 at 147.904 kb on + strand at 147.905 kb on - strand at 147.905 kb on - strand at 147.956 kb on - strand at 148.053 kb on - strand at 148.417 kb on + strand at 148.639 kb on + strand at 148.765 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2 remove 145,917 - GFF1954 0.66 -0.8 145,959 + GFF1954 0.68 -0.6 145,991 + GFF1954 0.70 -1.1 146,246 + GFF1954 0.82 -0.2 146,247 - GFF1954 0.82 -0.0 146,421 + GFF1954 0.90 +0.2 146,433 - -0.9 146,454 - +0.2 146,785 - GFF1955 0.11 +0.3 146,891 + GFF1955 0.19 +0.5 147,006 - GFF1955 0.28 +0.1 147,006 - GFF1955 0.28 +0.9 147,055 - GFF1955 0.32 -0.1 147,265 - GFF1955 0.48 -1.2 147,265 - GFF1955 0.48 -0.0 147,365 - GFF1955 0.55 +1.2 147,365 - GFF1955 0.55 +1.2 147,369 + GFF1955 0.55 +0.3 147,370 - GFF1955 0.56 +0.1 147,669 - GFF1955 0.78 +0.6 147,728 - GFF1955 0.83 +0.7 147,750 - GFF1955 0.85 +1.3 147,752 + GFF1955 0.85 -0.3 147,904 + +0.2 147,905 - +0.3 147,905 - +0.1 147,956 - +0.2 148,053 - -0.1 148,417 + +0.1 148,639 + -0.3 148,765 + +0.3
Or see this region's nucleotide sequence