Experiment: Acanthamoeba sp., muxed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1880 and GFF1881 are separated by 313 nucleotides GFF1881 and GFF1882 are separated by 2 nucleotides GFF1882 and GFF1883 overlap by 4 nucleotides GFF1883 and GFF1884 are separated by 10 nucleotides
GFF1880 - NADH-ubiquinone oxidoreductase chain M (EC 1.6.5.3), at 72,211 to 73,740
GFF1880
GFF1881 - NADH-ubiquinone oxidoreductase chain L (EC 1.6.5.3), at 74,054 to 75,889
GFF1881
GFF1882 - NADH-ubiquinone oxidoreductase chain K (EC 1.6.5.3), at 75,892 to 76,194
GFF1882
GFF1883 - NADH-ubiquinone oxidoreductase chain J (EC 1.6.5.3), at 76,191 to 76,745
GFF1883
GFF1884 - NADH-ubiquinone oxidoreductase chain I (EC 1.6.5.3), at 76,756 to 77,298
GFF1884
Position (kb) 74
75
76 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 73.212 kb on - strand, within GFF1880 at 73.474 kb on + strand, within GFF1880 at 73.506 kb on + strand, within GFF1880 at 73.506 kb on + strand, within GFF1880 at 73.736 kb on - strand at 73.745 kb on - strand at 73.887 kb on + strand at 73.887 kb on + strand at 73.888 kb on - strand at 73.888 kb on - strand at 73.896 kb on - strand at 74.011 kb on + strand at 74.115 kb on + strand at 74.116 kb on - strand at 74.116 kb on - strand at 74.116 kb on - strand at 74.128 kb on - strand at 74.252 kb on + strand, within GFF1881 at 74.253 kb on - strand, within GFF1881 at 74.263 kb on - strand, within GFF1881 at 74.289 kb on + strand, within GFF1881 at 74.290 kb on - strand, within GFF1881 at 74.968 kb on - strand, within GFF1881 at 75.021 kb on - strand, within GFF1881 at 75.028 kb on + strand, within GFF1881 at 75.028 kb on + strand, within GFF1881 at 75.331 kb on - strand, within GFF1881 at 75.331 kb on - strand, within GFF1881 at 75.359 kb on + strand, within GFF1881 at 75.427 kb on - strand, within GFF1881 at 75.445 kb on - strand, within GFF1881 at 75.478 kb on - strand, within GFF1881 at 75.478 kb on - strand, within GFF1881 at 75.538 kb on + strand, within GFF1881 at 75.539 kb on - strand, within GFF1881 at 75.541 kb on - strand, within GFF1881 at 75.579 kb on + strand, within GFF1881 at 75.872 kb on - strand at 75.911 kb on - strand at 76.010 kb on + strand, within GFF1882 at 76.010 kb on + strand, within GFF1882 at 76.010 kb on + strand, within GFF1882 at 76.011 kb on - strand, within GFF1882 at 76.011 kb on - strand, within GFF1882 at 76.105 kb on - strand, within GFF1882 at 76.153 kb on + strand, within GFF1882 at 76.310 kb on + strand, within GFF1883 at 76.311 kb on - strand, within GFF1883 at 76.311 kb on - strand, within GFF1883 at 76.336 kb on + strand, within GFF1883 at 76.421 kb on - strand, within GFF1883 at 76.600 kb on + strand, within GFF1883 at 76.600 kb on + strand, within GFF1883 at 76.601 kb on - strand, within GFF1883 at 76.655 kb on - strand, within GFF1883 at 76.679 kb on + strand, within GFF1883 at 76.728 kb on - strand at 76.757 kb on + strand at 76.857 kb on - strand, within GFF1884
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2 remove 73,212 - GFF1880 0.65 -1.9 73,474 + GFF1880 0.83 +0.7 73,506 + GFF1880 0.85 -2.6 73,506 + GFF1880 0.85 -0.8 73,736 - -0.5 73,745 - -1.4 73,887 + -1.9 73,887 + -1.3 73,888 - -0.9 73,888 - -1.2 73,896 - -0.8 74,011 + -1.3 74,115 + -0.8 74,116 - -1.0 74,116 - -3.0 74,116 - -2.6 74,128 - -1.0 74,252 + GFF1881 0.11 -1.5 74,253 - GFF1881 0.11 -1.1 74,263 - GFF1881 0.11 -0.1 74,289 + GFF1881 0.13 -0.3 74,290 - GFF1881 0.13 -1.9 74,968 - GFF1881 0.50 +1.1 75,021 - GFF1881 0.53 -0.1 75,028 + GFF1881 0.53 -0.3 75,028 + GFF1881 0.53 -1.5 75,331 - GFF1881 0.70 -0.8 75,331 - GFF1881 0.70 +0.3 75,359 + GFF1881 0.71 -0.9 75,427 - GFF1881 0.75 -1.1 75,445 - GFF1881 0.76 -1.3 75,478 - GFF1881 0.78 -2.3 75,478 - GFF1881 0.78 +0.9 75,538 + GFF1881 0.81 -0.7 75,539 - GFF1881 0.81 -2.2 75,541 - GFF1881 0.81 -1.8 75,579 + GFF1881 0.83 -0.6 75,872 - -1.2 75,911 - +0.5 76,010 + GFF1882 0.39 -1.3 76,010 + GFF1882 0.39 -1.6 76,010 + GFF1882 0.39 -1.0 76,011 - GFF1882 0.39 -1.4 76,011 - GFF1882 0.39 -2.0 76,105 - GFF1882 0.70 -1.5 76,153 + GFF1882 0.86 -0.9 76,310 + GFF1883 0.21 -1.2 76,311 - GFF1883 0.22 -0.6 76,311 - GFF1883 0.22 -1.5 76,336 + GFF1883 0.26 -0.9 76,421 - GFF1883 0.41 -0.9 76,600 + GFF1883 0.74 -1.0 76,600 + GFF1883 0.74 -2.1 76,601 - GFF1883 0.74 -1.3 76,655 - GFF1883 0.84 -1.3 76,679 + GFF1883 0.88 -1.2 76,728 - -1.4 76,757 + -0.7 76,857 - GFF1884 0.19 -1.5
Or see this region's nucleotide sequence