Experiment: Acanthamoeba sp., muxed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1648 and GFF1649 overlap by 4 nucleotides GFF1649 and GFF1650 are separated by 52 nucleotides GFF1650 and GFF1651 are separated by 40 nucleotides GFF1651 and GFF1652 overlap by 4 nucleotides
GFF1648 - Nicotinamidase/isochorismatase family protein, at 33,629 to 34,195
GFF1648
GFF1649 - FIG003003: hypothetical protein, at 34,192 to 35,010
GFF1649
GFF1650 - FIG01046209: inner membrane protein, at 35,063 to 35,458
GFF1650
GFF1651 - tRNA (uridine-5-oxyacetic acid methyl ester) 34 synthase, at 35,499 to 36,242
GFF1651
GFF1652 - tRNA (5-methoxyuridine) 34 synthase, at 36,239 to 37,210
GFF1652
Position (kb) 35
36 Strain fitness (log2 ratio) -1
0
1 at 34.138 kb on + strand, within GFF1648 at 34.199 kb on - strand at 34.566 kb on + strand, within GFF1649 at 34.567 kb on - strand, within GFF1649 at 34.578 kb on - strand, within GFF1649 at 34.641 kb on - strand, within GFF1649 at 34.685 kb on + strand, within GFF1649 at 34.686 kb on - strand, within GFF1649 at 35.026 kb on + strand at 35.027 kb on - strand at 35.078 kb on + strand at 35.144 kb on + strand, within GFF1650 at 35.288 kb on - strand, within GFF1650 at 35.424 kb on - strand at 35.424 kb on - strand at 35.424 kb on - strand at 35.426 kb on - strand at 35.498 kb on + strand at 35.498 kb on + strand at 35.499 kb on - strand at 35.614 kb on - strand, within GFF1651 at 35.644 kb on + strand, within GFF1651 at 35.679 kb on + strand, within GFF1651 at 35.727 kb on - strand, within GFF1651 at 35.765 kb on + strand, within GFF1651 at 35.811 kb on + strand, within GFF1651 at 35.822 kb on + strand, within GFF1651 at 36.066 kb on + strand, within GFF1651
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2 remove 34,138 + GFF1648 0.90 -0.7 34,199 - -0.4 34,566 + GFF1649 0.46 +0.5 34,567 - GFF1649 0.46 -1.7 34,578 - GFF1649 0.47 +0.4 34,641 - GFF1649 0.55 -0.1 34,685 + GFF1649 0.60 +0.0 34,686 - GFF1649 0.60 +0.5 35,026 + -0.4 35,027 - -0.2 35,078 + +0.1 35,144 + GFF1650 0.20 -0.5 35,288 - GFF1650 0.57 -0.4 35,424 - +0.3 35,424 - -0.4 35,424 - +0.2 35,426 - +0.6 35,498 + +0.5 35,498 + -0.4 35,499 - +0.2 35,614 - GFF1651 0.15 +0.1 35,644 + GFF1651 0.19 -0.3 35,679 + GFF1651 0.24 -1.6 35,727 - GFF1651 0.31 -0.6 35,765 + GFF1651 0.36 +0.5 35,811 + GFF1651 0.42 +0.1 35,822 + GFF1651 0.43 +0.4 36,066 + GFF1651 0.76 -0.4
Or see this region's nucleotide sequence