Experiment: Acanthamoeba sp., muxed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1643 and GFF1644 are separated by 1 nucleotides GFF1644 and GFF1645 are separated by 36 nucleotides GFF1645 and GFF1646 are separated by 29 nucleotides GFF1646 and GFF1647 are separated by 102 nucleotides
GFF1643 - hypothetical protein, at 29,048 to 29,647
GFF1643
GFF1644 - Crossover junction endodeoxyribonuclease RuvC (EC 3.1.22.4), at 29,649 to 30,170
GFF1644
GFF1645 - FIG000859: hypothetical protein YebC, at 30,207 to 30,947
GFF1645
GFF1646 - Dihydroneopterin triphosphate pyrophosphohydrolase type 2 (nudB), at 30,977 to 31,429
GFF1646
GFF1647 - Aspartyl-tRNA synthetase (EC 6.1.1.12), at 31,532 to 33,304
GFF1647
Position (kb)
30
31 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 29.249 kb on - strand, within GFF1643 at 29.378 kb on + strand, within GFF1643 at 29.420 kb on - strand, within GFF1643 at 29.423 kb on + strand, within GFF1643 at 29.526 kb on + strand, within GFF1643 at 29.549 kb on - strand, within GFF1643 at 30.455 kb on - strand, within GFF1645 at 30.455 kb on - strand, within GFF1645 at 30.657 kb on - strand, within GFF1645 at 30.689 kb on - strand, within GFF1645 at 30.689 kb on - strand, within GFF1645 at 30.933 kb on - strand at 30.933 kb on - strand at 30.936 kb on - strand at 31.089 kb on - strand, within GFF1646 at 31.520 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2 remove 29,249 - GFF1643 0.34 +0.2 29,378 + GFF1643 0.55 +0.2 29,420 - GFF1643 0.62 -0.3 29,423 + GFF1643 0.62 -0.1 29,526 + GFF1643 0.80 +0.9 29,549 - GFF1643 0.83 +0.3 30,455 - GFF1645 0.33 -0.4 30,455 - GFF1645 0.33 +0.1 30,657 - GFF1645 0.61 -0.4 30,689 - GFF1645 0.65 -3.4 30,689 - GFF1645 0.65 -0.3 30,933 - -0.8 30,933 - -0.6 30,936 - -0.3 31,089 - GFF1646 0.25 +0.2 31,520 - -0.1
Or see this region's nucleotide sequence