Experiment: Acanthamoeba sp., muxed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1581 and GFF1582 are separated by 14 nucleotides GFF1582 and GFF1583 are separated by 98 nucleotides GFF1583 and GFF1584 overlap by 4 nucleotides GFF1584 and GFF1585 overlap by 28 nucleotides GFF1585 and GFF1586 are separated by 10 nucleotides
GFF1581 - 4-carboxymuconolactone decarboxylase domain/alkylhydroperoxidase AhpD family core domain protein, at 39,333 to 39,767
GFF1581
GFF1582 - FIG01046421: hypothetical protein, at 39,782 to 39,940
GFF1582
GFF1583 - Glutaredoxin-like protein NrdH, required for reduction of Ribonucleotide reductase class Ib, at 40,039 to 40,284
GFF1583
GFF1584 - Ribonucleotide reduction protein NrdI, at 40,281 to 40,691
GFF1584
GFF1585 - Ribonucleotide reductase of class Ib (aerobic), alpha subunit (EC 1.17.4.1), at 40,664 to 42,808
GFF1585
GFF1586 - Ribonucleotide reductase of class Ib (aerobic), beta subunit (EC 1.17.4.1), at 42,819 to 43,778
GFF1586
Position (kb) 40
41
42
43 Strain fitness (log2 ratio) -2
-1
0
1 at 40.296 kb on + strand at 40.319 kb on + strand at 40.362 kb on - strand, within GFF1584 at 40.404 kb on + strand, within GFF1584 at 40.654 kb on + strand at 40.756 kb on + strand at 40.756 kb on + strand at 40.875 kb on - strand at 40.997 kb on + strand, within GFF1585 at 40.998 kb on - strand, within GFF1585 at 41.158 kb on - strand, within GFF1585 at 41.435 kb on + strand, within GFF1585 at 41.605 kb on - strand, within GFF1585 at 41.605 kb on - strand, within GFF1585 at 41.665 kb on - strand, within GFF1585 at 41.696 kb on - strand, within GFF1585 at 41.756 kb on + strand, within GFF1585 at 41.757 kb on - strand, within GFF1585 at 41.757 kb on - strand, within GFF1585 at 41.812 kb on + strand, within GFF1585 at 41.880 kb on - strand, within GFF1585 at 41.967 kb on + strand, within GFF1585 at 42.013 kb on + strand, within GFF1585 at 42.014 kb on - strand, within GFF1585 at 42.016 kb on - strand, within GFF1585 at 42.110 kb on - strand, within GFF1585 at 42.166 kb on + strand, within GFF1585 at 42.166 kb on + strand, within GFF1585 at 42.184 kb on + strand, within GFF1585 at 42.185 kb on - strand, within GFF1585 at 42.319 kb on - strand, within GFF1585 at 42.398 kb on + strand, within GFF1585 at 42.398 kb on + strand, within GFF1585 at 42.398 kb on + strand, within GFF1585 at 42.399 kb on - strand, within GFF1585 at 42.448 kb on + strand, within GFF1585 at 42.541 kb on + strand, within GFF1585 at 42.567 kb on - strand, within GFF1585 at 42.607 kb on + strand at 42.608 kb on - strand at 42.707 kb on + strand at 42.725 kb on - strand at 42.728 kb on - strand at 42.728 kb on - strand at 42.740 kb on + strand at 42.806 kb on + strand at 42.819 kb on - strand at 42.932 kb on - strand, within GFF1586 at 43.008 kb on - strand, within GFF1586 at 43.132 kb on - strand, within GFF1586 at 43.139 kb on + strand, within GFF1586 at 43.289 kb on - strand, within GFF1586 at 43.507 kb on - strand, within GFF1586 at 43.537 kb on + strand, within GFF1586 at 43.584 kb on + strand, within GFF1586 at 43.595 kb on + strand, within GFF1586 at 43.729 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2 remove 40,296 + +0.2 40,319 + -0.1 40,362 - GFF1584 0.20 +0.6 40,404 + GFF1584 0.30 -0.6 40,654 + +0.7 40,756 + -0.2 40,756 + -0.2 40,875 - -1.2 40,997 + GFF1585 0.16 +0.2 40,998 - GFF1585 0.16 -0.7 41,158 - GFF1585 0.23 +0.4 41,435 + GFF1585 0.36 -0.2 41,605 - GFF1585 0.44 -0.0 41,605 - GFF1585 0.44 +0.5 41,665 - GFF1585 0.47 +1.0 41,696 - GFF1585 0.48 -0.6 41,756 + GFF1585 0.51 -0.3 41,757 - GFF1585 0.51 +0.5 41,757 - GFF1585 0.51 +0.1 41,812 + GFF1585 0.54 +1.2 41,880 - GFF1585 0.57 -0.1 41,967 + GFF1585 0.61 -0.0 42,013 + GFF1585 0.63 -0.0 42,014 - GFF1585 0.63 -0.2 42,016 - GFF1585 0.63 -0.1 42,110 - GFF1585 0.67 -0.4 42,166 + GFF1585 0.70 -2.7 42,166 + GFF1585 0.70 +0.8 42,184 + GFF1585 0.71 +0.4 42,185 - GFF1585 0.71 +0.1 42,319 - GFF1585 0.77 +0.5 42,398 + GFF1585 0.81 -0.1 42,398 + GFF1585 0.81 +0.0 42,398 + GFF1585 0.81 +0.1 42,399 - GFF1585 0.81 +0.7 42,448 + GFF1585 0.83 +0.2 42,541 + GFF1585 0.88 -0.3 42,567 - GFF1585 0.89 +0.4 42,607 + -0.0 42,608 - -0.2 42,707 + -0.1 42,725 - -0.7 42,728 - +0.1 42,728 - -0.1 42,740 + -1.2 42,806 + -0.1 42,819 - -0.6 42,932 - GFF1586 0.12 -1.0 43,008 - GFF1586 0.20 +0.0 43,132 - GFF1586 0.33 +0.5 43,139 + GFF1586 0.33 +0.3 43,289 - GFF1586 0.49 +0.0 43,507 - GFF1586 0.72 +0.7 43,537 + GFF1586 0.75 +0.6 43,584 + GFF1586 0.80 -0.1 43,595 + GFF1586 0.81 -0.1 43,729 - +0.0
Or see this region's nucleotide sequence