Experiment: Acanthamoeba sp., muxed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1450 and GFF1451 overlap by 4 nucleotides GFF1451 and GFF1452 are separated by 245 nucleotides
GFF1450 - Acetate permease ActP (cation/acetate symporter), at 111,639 to 113,288
GFF1450
GFF1451 - Putative membrane protein, clustering with ActP, at 113,285 to 113,599
GFF1451
GFF1452 - Acetyl-coenzyme A synthetase (EC 6.2.1.1), at 113,845 to 115,803
GFF1452
Position (kb) 113
114 Strain fitness (log2 ratio) -1
0
1 at 112.350 kb on - strand, within GFF1450 at 112.364 kb on - strand, within GFF1450 at 112.705 kb on + strand, within GFF1450 at 112.705 kb on + strand, within GFF1450 at 112.706 kb on - strand, within GFF1450 at 112.706 kb on - strand, within GFF1450 at 112.706 kb on - strand, within GFF1450 at 112.706 kb on - strand, within GFF1450 at 112.747 kb on + strand, within GFF1450 at 112.748 kb on - strand, within GFF1450 at 112.807 kb on + strand, within GFF1450 at 112.822 kb on - strand, within GFF1450 at 112.822 kb on - strand, within GFF1450 at 112.873 kb on + strand, within GFF1450 at 112.874 kb on - strand, within GFF1450 at 112.895 kb on + strand, within GFF1450 at 112.897 kb on + strand, within GFF1450 at 112.972 kb on + strand, within GFF1450 at 112.998 kb on - strand, within GFF1450 at 113.039 kb on + strand, within GFF1450 at 113.040 kb on - strand, within GFF1450 at 113.041 kb on + strand, within GFF1450 at 113.041 kb on + strand, within GFF1450 at 113.042 kb on - strand, within GFF1450 at 113.082 kb on - strand, within GFF1450 at 113.099 kb on - strand, within GFF1450 at 113.099 kb on - strand, within GFF1450 at 113.355 kb on + strand, within GFF1451 at 113.355 kb on + strand, within GFF1451 at 113.491 kb on - strand, within GFF1451 at 113.574 kb on - strand at 113.677 kb on - strand at 113.690 kb on + strand at 113.840 kb on + strand at 113.840 kb on + strand at 113.840 kb on + strand at 113.841 kb on - strand at 113.973 kb on + strand at 114.051 kb on + strand, within GFF1452 at 114.051 kb on + strand, within GFF1452 at 114.052 kb on - strand, within GFF1452 at 114.056 kb on - strand, within GFF1452 at 114.119 kb on + strand, within GFF1452 at 114.119 kb on + strand, within GFF1452 at 114.157 kb on - strand, within GFF1452 at 114.245 kb on - strand, within GFF1452 at 114.317 kb on + strand, within GFF1452 at 114.322 kb on - strand, within GFF1452 at 114.328 kb on - strand, within GFF1452 at 114.334 kb on - strand, within GFF1452 at 114.541 kb on - strand, within GFF1452
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2 remove 112,350 - GFF1450 0.43 +0.2 112,364 - GFF1450 0.44 -0.0 112,705 + GFF1450 0.65 -0.4 112,705 + GFF1450 0.65 -0.1 112,706 - GFF1450 0.65 +0.6 112,706 - GFF1450 0.65 -0.5 112,706 - GFF1450 0.65 +0.1 112,706 - GFF1450 0.65 -0.2 112,747 + GFF1450 0.67 +0.4 112,748 - GFF1450 0.67 -0.2 112,807 + GFF1450 0.71 +0.0 112,822 - GFF1450 0.72 -0.0 112,822 - GFF1450 0.72 -0.0 112,873 + GFF1450 0.75 -0.1 112,874 - GFF1450 0.75 -0.9 112,895 + GFF1450 0.76 +0.3 112,897 + GFF1450 0.76 -0.3 112,972 + GFF1450 0.81 +0.3 112,998 - GFF1450 0.82 -0.1 113,039 + GFF1450 0.85 +0.3 113,040 - GFF1450 0.85 +0.1 113,041 + GFF1450 0.85 +0.1 113,041 + GFF1450 0.85 -0.3 113,042 - GFF1450 0.85 +0.1 113,082 - GFF1450 0.87 +0.0 113,099 - GFF1450 0.88 -0.2 113,099 - GFF1450 0.88 -0.2 113,355 + GFF1451 0.22 -0.9 113,355 + GFF1451 0.22 -0.1 113,491 - GFF1451 0.65 -0.7 113,574 - +0.3 113,677 - +0.1 113,690 + +0.6 113,840 + -0.0 113,840 + +0.1 113,840 + +0.1 113,841 - +0.0 113,973 + -0.9 114,051 + GFF1452 0.11 +0.3 114,051 + GFF1452 0.11 +0.5 114,052 - GFF1452 0.11 +0.4 114,056 - GFF1452 0.11 +0.1 114,119 + GFF1452 0.14 +0.2 114,119 + GFF1452 0.14 -0.2 114,157 - GFF1452 0.16 -0.4 114,245 - GFF1452 0.20 +0.2 114,317 + GFF1452 0.24 -0.0 114,322 - GFF1452 0.24 -0.2 114,328 - GFF1452 0.25 +0.2 114,334 - GFF1452 0.25 -0.9 114,541 - GFF1452 0.36 +0.6
Or see this region's nucleotide sequence