Experiment: Acanthamoeba sp., muxed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1447 and GFF1448 are separated by 103 nucleotides GFF1448 and GFF1449 overlap by 8 nucleotides GFF1449 and GFF1450 are separated by 38 nucleotides
GFF1447 - LysR family regulatory protein CidR, at 109,517 to 110,404
GFF1447
GFF1448 - Holin-like protein CidA, at 110,508 to 110,918
GFF1448
GFF1449 - CidA-associated membrane protein CidB, at 110,911 to 111,600
GFF1449
GFF1450 - Acetate permease ActP (cation/acetate symporter), at 111,639 to 113,288
GFF1450
Position (kb)
110
111 Strain fitness (log2 ratio)
-1
0
1 at 109.582 kb on - strand at 109.766 kb on + strand, within GFF1447 at 109.767 kb on - strand, within GFF1447 at 109.767 kb on - strand, within GFF1447 at 109.784 kb on + strand, within GFF1447 at 109.785 kb on - strand, within GFF1447 at 109.785 kb on - strand, within GFF1447 at 109.825 kb on - strand, within GFF1447 at 109.828 kb on + strand, within GFF1447 at 109.829 kb on - strand, within GFF1447 at 109.829 kb on - strand, within GFF1447 at 109.829 kb on - strand, within GFF1447 at 109.829 kb on - strand, within GFF1447 at 110.013 kb on - strand, within GFF1447 at 110.152 kb on + strand, within GFF1447 at 110.240 kb on + strand, within GFF1447 at 110.241 kb on - strand, within GFF1447 at 110.264 kb on + strand, within GFF1447 at 110.270 kb on - strand, within GFF1447 at 110.275 kb on + strand, within GFF1447 at 110.276 kb on - strand, within GFF1447 at 110.276 kb on - strand, within GFF1447 at 110.330 kb on + strand at 110.382 kb on + strand at 110.438 kb on + strand at 110.470 kb on - strand at 110.470 kb on - strand at 110.531 kb on + strand at 110.591 kb on - strand, within GFF1448 at 110.663 kb on + strand, within GFF1448 at 110.663 kb on + strand, within GFF1448 at 110.664 kb on - strand, within GFF1448 at 110.699 kb on + strand, within GFF1448 at 110.782 kb on - strand, within GFF1448 at 110.842 kb on + strand, within GFF1448 at 110.842 kb on + strand, within GFF1448 at 110.849 kb on - strand, within GFF1448 at 110.890 kb on - strand at 110.890 kb on - strand at 110.934 kb on - strand at 110.984 kb on - strand, within GFF1449 at 111.017 kb on + strand, within GFF1449 at 111.064 kb on - strand, within GFF1449 at 111.064 kb on - strand, within GFF1449 at 111.064 kb on - strand, within GFF1449 at 111.079 kb on + strand, within GFF1449 at 111.291 kb on - strand, within GFF1449 at 111.537 kb on - strand at 111.640 kb on + strand at 111.641 kb on - strand at 111.779 kb on + strand at 111.905 kb on + strand, within GFF1450
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2 remove 109,582 - +0.5 109,766 + GFF1447 0.28 -0.1 109,767 - GFF1447 0.28 +0.2 109,767 - GFF1447 0.28 -0.0 109,784 + GFF1447 0.30 +0.0 109,785 - GFF1447 0.30 +0.2 109,785 - GFF1447 0.30 -0.6 109,825 - GFF1447 0.35 -0.8 109,828 + GFF1447 0.35 +0.3 109,829 - GFF1447 0.35 +0.5 109,829 - GFF1447 0.35 -0.0 109,829 - GFF1447 0.35 +0.1 109,829 - GFF1447 0.35 -0.3 110,013 - GFF1447 0.56 +0.3 110,152 + GFF1447 0.72 +0.3 110,240 + GFF1447 0.81 -0.1 110,241 - GFF1447 0.82 -0.3 110,264 + GFF1447 0.84 +0.5 110,270 - GFF1447 0.85 +0.2 110,275 + GFF1447 0.85 -0.7 110,276 - GFF1447 0.85 +0.3 110,276 - GFF1447 0.85 +0.1 110,330 + +0.4 110,382 + -0.1 110,438 + -0.2 110,470 - -0.2 110,470 - -0.2 110,531 + +0.3 110,591 - GFF1448 0.20 -0.0 110,663 + GFF1448 0.38 -0.3 110,663 + GFF1448 0.38 +0.3 110,664 - GFF1448 0.38 +0.1 110,699 + GFF1448 0.46 -0.5 110,782 - GFF1448 0.67 -1.4 110,842 + GFF1448 0.81 -0.6 110,842 + GFF1448 0.81 +0.1 110,849 - GFF1448 0.83 -0.9 110,890 - -0.5 110,890 - -0.0 110,934 - -0.8 110,984 - GFF1449 0.11 -0.2 111,017 + GFF1449 0.15 +0.2 111,064 - GFF1449 0.22 -0.3 111,064 - GFF1449 0.22 +0.3 111,064 - GFF1449 0.22 +0.5 111,079 + GFF1449 0.24 +0.2 111,291 - GFF1449 0.55 +0.5 111,537 - +0.6 111,640 + -0.3 111,641 - -0.1 111,779 + -0.3 111,905 + GFF1450 0.16 +0.3
Or see this region's nucleotide sequence