Experiment: Acanthamoeba sp., muxed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1410 and GFF1411 are separated by 170 nucleotides GFF1411 and GFF1412 are separated by 108 nucleotides GFF1412 and GFF1413 are separated by 178 nucleotides
GFF1410 - Glycerol-3-phosphate acyltransferase (EC 2.3.1.15), at 56,311 to 58,731
GFF1410
GFF1411 - Diacylglycerol kinase (EC 2.7.1.107), at 58,902 to 59,270
GFF1411
GFF1412 - SOS-response repressor and protease LexA (EC 3.4.21.88), at 59,379 to 59,987
GFF1412
GFF1413 - DNA-damage-inducible protein F, at 60,166 to 61,491
GFF1413
Position (kb)
59
60 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 58.862 kb on - strand at 58.862 kb on - strand at 58.862 kb on - strand at 58.862 kb on - strand at 58.864 kb on - strand at 58.911 kb on - strand at 58.951 kb on - strand, within GFF1411 at 59.080 kb on + strand, within GFF1411 at 59.092 kb on + strand, within GFF1411 at 59.097 kb on - strand, within GFF1411 at 59.097 kb on - strand, within GFF1411 at 59.127 kb on - strand, within GFF1411 at 59.209 kb on + strand, within GFF1411 at 59.209 kb on + strand, within GFF1411 at 59.986 kb on - strand at 60.109 kb on + strand at 60.228 kb on + strand at 60.359 kb on + strand, within GFF1413 at 60.364 kb on - strand, within GFF1413 at 60.509 kb on + strand, within GFF1413 at 60.513 kb on + strand, within GFF1413 at 60.514 kb on - strand, within GFF1413 at 60.514 kb on - strand, within GFF1413 at 60.770 kb on + strand, within GFF1413 at 60.771 kb on - strand, within GFF1413 at 60.792 kb on - strand, within GFF1413 at 60.796 kb on - strand, within GFF1413 at 60.828 kb on + strand, within GFF1413 at 60.832 kb on + strand, within GFF1413 at 60.834 kb on - strand, within GFF1413 at 60.971 kb on + strand, within GFF1413
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2 remove 58,862 - -0.1 58,862 - -0.9 58,862 - +0.3 58,862 - -0.1 58,864 - -2.4 58,911 - -4.8 58,951 - GFF1411 0.13 -3.6 59,080 + GFF1411 0.48 -2.4 59,092 + GFF1411 0.51 -2.2 59,097 - GFF1411 0.53 -2.0 59,097 - GFF1411 0.53 -2.6 59,127 - GFF1411 0.61 -3.3 59,209 + GFF1411 0.83 -1.3 59,209 + GFF1411 0.83 -4.0 59,986 - +1.7 60,109 + -0.5 60,228 + -0.6 60,359 + GFF1413 0.15 -0.1 60,364 - GFF1413 0.15 +0.1 60,509 + GFF1413 0.26 +0.1 60,513 + GFF1413 0.26 -0.1 60,514 - GFF1413 0.26 +0.2 60,514 - GFF1413 0.26 +0.4 60,770 + GFF1413 0.46 -0.3 60,771 - GFF1413 0.46 -1.2 60,792 - GFF1413 0.47 +0.4 60,796 - GFF1413 0.48 -1.2 60,828 + GFF1413 0.50 +0.1 60,832 + GFF1413 0.50 +0.2 60,834 - GFF1413 0.50 -0.8 60,971 + GFF1413 0.61 +0.1
Or see this region's nucleotide sequence