Experiment: Acanthamoeba sp., muxed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1352 and GFF1353 are separated by 3 nucleotides GFF1353 and GFF1354 overlap by 25 nucleotides GFF1354 and GFF1355 overlap by 37 nucleotides GFF1355 and GFF1356 overlap by 17 nucleotides
GFF1352 - Isocitrate lyase (EC 4.1.3.1), at 3,800 to 5,104
GFF1352
GFF1353 - hypothetical protein, at 5,108 to 5,230
GFF1353
GFF1354 - Isocitrate dehydrogenase phosphatase (EC 2.7.11.5)/kinase (EC 3.1.3.-), at 5,206 to 6,957
GFF1354
GFF1355 - hypothetical protein, at 6,921 to 7,355
GFF1355
GFF1356 - Acetate operon repressor, at 7,339 to 8,163
GFF1356
Position (kb)
5
6
7 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4.247 kb on + strand, within GFF1352 at 4.555 kb on + strand, within GFF1352 at 4.586 kb on + strand, within GFF1352 at 4.673 kb on - strand, within GFF1352 at 4.673 kb on - strand, within GFF1352 at 4.673 kb on - strand, within GFF1352 at 4.747 kb on + strand, within GFF1352 at 4.839 kb on - strand, within GFF1352 at 4.846 kb on + strand, within GFF1352 at 4.904 kb on + strand, within GFF1352 at 4.932 kb on - strand, within GFF1352 at 5.019 kb on - strand at 5.019 kb on - strand at 5.148 kb on + strand, within GFF1353 at 5.406 kb on + strand, within GFF1354 at 5.407 kb on - strand, within GFF1354 at 5.407 kb on - strand, within GFF1354 at 5.710 kb on - strand, within GFF1354 at 5.730 kb on + strand, within GFF1354 at 5.731 kb on - strand, within GFF1354 at 5.809 kb on + strand, within GFF1354 at 5.844 kb on - strand, within GFF1354 at 5.917 kb on - strand, within GFF1354 at 5.977 kb on - strand, within GFF1354 at 5.977 kb on - strand, within GFF1354 at 5.984 kb on - strand, within GFF1354 at 6.056 kb on - strand, within GFF1354 at 6.058 kb on - strand, within GFF1354 at 6.110 kb on + strand, within GFF1354 at 6.111 kb on - strand, within GFF1354 at 6.426 kb on + strand, within GFF1354 at 6.427 kb on - strand, within GFF1354 at 6.447 kb on + strand, within GFF1354 at 6.448 kb on - strand, within GFF1354 at 6.526 kb on + strand, within GFF1354 at 6.576 kb on + strand, within GFF1354 at 6.625 kb on + strand, within GFF1354 at 6.632 kb on - strand, within GFF1354 at 6.647 kb on - strand, within GFF1354 at 6.723 kb on + strand, within GFF1354 at 6.724 kb on - strand, within GFF1354 at 6.875 kb on + strand at 6.876 kb on - strand at 6.876 kb on - strand at 6.886 kb on + strand at 6.886 kb on + strand at 6.886 kb on + strand at 6.886 kb on + strand at 6.887 kb on - strand at 6.887 kb on - strand at 6.887 kb on - strand at 6.913 kb on - strand at 6.939 kb on + strand at 6.940 kb on - strand at 7.082 kb on + strand, within GFF1355 at 7.156 kb on + strand, within GFF1355 at 7.196 kb on + strand, within GFF1355 at 7.208 kb on + strand, within GFF1355 at 7.355 kb on + strand at 7.355 kb on + strand at 7.485 kb on + strand, within GFF1356 at 7.605 kb on + strand, within GFF1356
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2 remove 4,247 + GFF1352 0.34 +0.8 4,555 + GFF1352 0.58 +0.1 4,586 + GFF1352 0.60 +0.1 4,673 - GFF1352 0.67 +0.6 4,673 - GFF1352 0.67 +0.5 4,673 - GFF1352 0.67 +0.2 4,747 + GFF1352 0.73 +0.3 4,839 - GFF1352 0.80 -0.0 4,846 + GFF1352 0.80 -0.2 4,904 + GFF1352 0.85 +0.5 4,932 - GFF1352 0.87 -0.2 5,019 - -0.4 5,019 - +0.2 5,148 + GFF1353 0.33 +0.7 5,406 + GFF1354 0.11 +0.2 5,407 - GFF1354 0.11 +0.9 5,407 - GFF1354 0.11 +0.1 5,710 - GFF1354 0.29 +0.2 5,730 + GFF1354 0.30 -0.4 5,731 - GFF1354 0.30 +0.2 5,809 + GFF1354 0.34 -0.1 5,844 - GFF1354 0.36 +2.0 5,917 - GFF1354 0.41 +1.2 5,977 - GFF1354 0.44 -0.0 5,977 - GFF1354 0.44 +0.1 5,984 - GFF1354 0.44 -0.3 6,056 - GFF1354 0.49 -0.7 6,058 - GFF1354 0.49 +0.1 6,110 + GFF1354 0.52 +0.5 6,111 - GFF1354 0.52 -0.6 6,426 + GFF1354 0.70 +0.6 6,427 - GFF1354 0.70 +0.4 6,447 + GFF1354 0.71 -0.3 6,448 - GFF1354 0.71 -1.7 6,526 + GFF1354 0.75 -0.2 6,576 + GFF1354 0.78 +0.0 6,625 + GFF1354 0.81 -0.3 6,632 - GFF1354 0.81 +0.0 6,647 - GFF1354 0.82 +0.4 6,723 + GFF1354 0.87 +0.2 6,724 - GFF1354 0.87 +0.0 6,875 + -2.7 6,876 - +0.5 6,876 - -0.2 6,886 + +0.4 6,886 + -0.9 6,886 + -0.2 6,886 + -0.2 6,887 - +0.5 6,887 - -0.3 6,887 - -0.7 6,913 - -0.2 6,939 + -0.1 6,940 - -0.0 7,082 + GFF1355 0.37 +1.5 7,156 + GFF1355 0.54 +0.5 7,196 + GFF1355 0.63 +0.1 7,208 + GFF1355 0.66 -0.3 7,355 + +0.4 7,355 + -0.9 7,485 + GFF1356 0.18 -1.0 7,605 + GFF1356 0.32 -1.1
Or see this region's nucleotide sequence