Experiment: Acanthamoeba sp., muxed culture-1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF724 and GFF725 overlap by 1 nucleotides GFF725 and GFF726 are separated by 202 nucleotides
GFF724 - Molybdopterin biosynthesis protein MoeB, at 82,026 to 82,775
GFF724
GFF725 - Molybdopterin biosynthesis protein MoeA, at 82,775 to 84,010
GFF725
GFF726 - Isoaspartyl aminopeptidase (EC 3.4.19.5) @ Asp-X dipeptidase, at 84,213 to 85,154
GFF726
Position (kb) 82
83
84
85 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 81.857 kb on - strand at 81.892 kb on - strand at 81.969 kb on - strand at 81.979 kb on - strand at 82.006 kb on + strand at 82.099 kb on + strand at 82.100 kb on - strand at 82.100 kb on - strand at 82.100 kb on - strand at 82.149 kb on - strand, within GFF724 at 82.283 kb on + strand, within GFF724 at 82.283 kb on + strand, within GFF724 at 82.330 kb on - strand, within GFF724 at 82.346 kb on + strand, within GFF724 at 82.349 kb on - strand, within GFF724 at 82.374 kb on + strand, within GFF724 at 82.374 kb on + strand, within GFF724 at 82.375 kb on - strand, within GFF724 at 82.434 kb on - strand, within GFF724 at 82.467 kb on - strand, within GFF724 at 82.475 kb on + strand, within GFF724 at 82.475 kb on + strand, within GFF724 at 82.515 kb on + strand, within GFF724 at 82.727 kb on + strand at 82.945 kb on + strand, within GFF725 at 83.137 kb on + strand, within GFF725 at 83.137 kb on + strand, within GFF725 at 83.138 kb on - strand, within GFF725 at 83.138 kb on - strand, within GFF725 at 83.138 kb on - strand, within GFF725 at 83.232 kb on + strand, within GFF725 at 83.232 kb on + strand, within GFF725 at 83.267 kb on + strand, within GFF725 at 83.267 kb on + strand, within GFF725 at 83.268 kb on - strand, within GFF725 at 83.336 kb on - strand, within GFF725 at 83.386 kb on + strand, within GFF725 at 83.387 kb on - strand, within GFF725 at 83.387 kb on - strand, within GFF725 at 83.482 kb on + strand, within GFF725 at 83.540 kb on - strand, within GFF725 at 83.950 kb on + strand at 84.017 kb on - strand at 84.017 kb on - strand at 84.045 kb on + strand at 84.048 kb on + strand at 84.049 kb on - strand at 84.049 kb on - strand at 84.049 kb on - strand at 84.073 kb on + strand at 84.110 kb on - strand at 84.114 kb on - strand at 84.119 kb on - strand at 84.131 kb on - strand at 84.133 kb on - strand at 84.174 kb on + strand at 84.174 kb on + strand at 84.289 kb on + strand at 84.290 kb on - strand at 84.295 kb on - strand at 84.367 kb on + strand, within GFF726 at 84.367 kb on + strand, within GFF726 at 84.432 kb on + strand, within GFF726 at 84.433 kb on - strand, within GFF726 at 84.571 kb on + strand, within GFF726 at 84.572 kb on - strand, within GFF726 at 84.819 kb on - strand, within GFF726 at 84.819 kb on - strand, within GFF726
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-1 remove 81,857 - +0.8 81,892 - -1.0 81,969 - +0.0 81,979 - -0.3 82,006 + +0.5 82,099 + -0.4 82,100 - -0.0 82,100 - -1.4 82,100 - -0.7 82,149 - GFF724 0.16 -0.4 82,283 + GFF724 0.34 -0.3 82,283 + GFF724 0.34 +1.1 82,330 - GFF724 0.41 -0.5 82,346 + GFF724 0.43 -2.9 82,349 - GFF724 0.43 -0.2 82,374 + GFF724 0.46 -0.2 82,374 + GFF724 0.46 -0.2 82,375 - GFF724 0.47 -0.4 82,434 - GFF724 0.54 +0.0 82,467 - GFF724 0.59 -0.9 82,475 + GFF724 0.60 -0.1 82,475 + GFF724 0.60 -1.4 82,515 + GFF724 0.65 +0.1 82,727 + -0.2 82,945 + GFF725 0.14 +0.1 83,137 + GFF725 0.29 -1.0 83,137 + GFF725 0.29 -0.1 83,138 - GFF725 0.29 -0.3 83,138 - GFF725 0.29 -0.2 83,138 - GFF725 0.29 -0.2 83,232 + GFF725 0.37 -0.3 83,232 + GFF725 0.37 +0.2 83,267 + GFF725 0.40 +0.1 83,267 + GFF725 0.40 -0.6 83,268 - GFF725 0.40 -0.6 83,336 - GFF725 0.45 +1.5 83,386 + GFF725 0.49 +0.0 83,387 - GFF725 0.50 -0.7 83,387 - GFF725 0.50 -0.8 83,482 + GFF725 0.57 -0.0 83,540 - GFF725 0.62 -0.3 83,950 + -0.2 84,017 - -0.5 84,017 - -0.5 84,045 + +0.9 84,048 + -1.3 84,049 - +0.6 84,049 - +0.8 84,049 - +0.3 84,073 + -1.0 84,110 - -0.2 84,114 - -0.7 84,119 - +0.2 84,131 - +0.1 84,133 - -0.5 84,174 + -0.1 84,174 + -0.2 84,289 + -0.1 84,290 - -0.9 84,295 - +0.3 84,367 + GFF726 0.16 +0.3 84,367 + GFF726 0.16 +0.1 84,432 + GFF726 0.23 -0.1 84,433 - GFF726 0.23 +0.3 84,571 + GFF726 0.38 +0.2 84,572 - GFF726 0.38 +0.3 84,819 - GFF726 0.64 +0.8 84,819 - GFF726 0.64 +1.2
Or see this region's nucleotide sequence