Experiment: Acanthamoeba sp., muxed culture-1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF585 and GFF586 are separated by 9 nucleotides GFF586 and GFF587 are separated by 46 nucleotides GFF587 and GFF588 are separated by 198 nucleotides GFF588 and GFF589 are separated by 75 nucleotides
GFF585 - Phosphate ABC transporter, periplasmic phosphate-binding protein PstS (TC 3.A.1.7.1), at 320,778 to 322,238
GFF585
GFF586 - Putative inner membrane protein, at 322,248 to 322,793
GFF586
GFF587 - Aerobic glycerol-3-phosphate dehydrogenase (EC 1.1.5.3), at 322,840 to 324,348
GFF587
GFF588 - Thiosulfate sulfurtransferase GlpE (EC 2.8.1.1), at 324,547 to 324,873
GFF588
GFF589 - GlpG protein (membrane protein of glp regulon), at 324,949 to 325,779
GFF589
Position (kb) 322
323
324
325 Strain fitness (log2 ratio) -2
-1
0
1 at 321.877 kb on + strand, within GFF585 at 322.002 kb on + strand, within GFF585 at 322.002 kb on + strand, within GFF585 at 322.034 kb on + strand, within GFF585 at 322.034 kb on + strand, within GFF585 at 322.092 kb on + strand, within GFF585 at 322.099 kb on - strand at 322.114 kb on - strand at 322.115 kb on + strand at 322.116 kb on - strand at 322.209 kb on + strand at 322.210 kb on - strand at 322.210 kb on - strand at 322.343 kb on + strand, within GFF586 at 322.349 kb on + strand, within GFF586 at 322.349 kb on + strand, within GFF586 at 322.438 kb on + strand, within GFF586 at 322.642 kb on - strand, within GFF586 at 322.670 kb on + strand, within GFF586 at 322.733 kb on - strand, within GFF586 at 322.751 kb on + strand at 322.752 kb on - strand at 322.763 kb on + strand at 322.763 kb on + strand at 322.800 kb on + strand at 322.801 kb on - strand at 322.801 kb on - strand at 322.822 kb on - strand at 322.842 kb on - strand at 322.950 kb on + strand at 323.100 kb on - strand, within GFF587 at 323.463 kb on - strand, within GFF587 at 323.524 kb on - strand, within GFF587 at 323.525 kb on + strand, within GFF587 at 323.784 kb on + strand, within GFF587 at 323.865 kb on + strand, within GFF587 at 324.027 kb on - strand, within GFF587 at 324.191 kb on - strand, within GFF587 at 324.451 kb on + strand at 324.452 kb on - strand at 324.455 kb on + strand at 324.455 kb on + strand at 324.456 kb on - strand at 324.456 kb on - strand at 324.457 kb on + strand at 324.476 kb on + strand at 324.489 kb on + strand at 324.490 kb on - strand at 324.512 kb on - strand at 324.564 kb on + strand at 324.564 kb on + strand at 324.564 kb on + strand at 324.565 kb on - strand at 324.584 kb on - strand, within GFF588 at 324.735 kb on - strand, within GFF588 at 324.743 kb on - strand, within GFF588 at 324.756 kb on + strand, within GFF588 at 324.776 kb on - strand, within GFF588 at 324.793 kb on + strand, within GFF588 at 324.909 kb on - strand at 324.910 kb on + strand at 324.910 kb on + strand at 324.910 kb on + strand at 324.910 kb on + strand at 324.910 kb on + strand at 324.910 kb on + strand at 324.910 kb on + strand at 324.910 kb on + strand at 324.910 kb on + strand at 324.911 kb on - strand at 324.911 kb on - strand at 324.911 kb on - strand at 324.911 kb on - strand at 324.911 kb on - strand at 324.911 kb on - strand at 324.911 kb on - strand at 324.911 kb on - strand at 324.911 kb on - strand at 324.912 kb on + strand at 324.912 kb on + strand at 324.913 kb on - strand at 324.913 kb on - strand at 324.913 kb on - strand at 324.913 kb on - strand at 324.927 kb on + strand at 325.004 kb on - strand at 325.029 kb on + strand at 325.056 kb on + strand, within GFF589 at 325.270 kb on + strand, within GFF589 at 325.270 kb on + strand, within GFF589 at 325.271 kb on - strand, within GFF589
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-1 remove 321,877 + GFF585 0.75 -0.1 322,002 + GFF585 0.84 +0.1 322,002 + GFF585 0.84 +0.2 322,034 + GFF585 0.86 +0.7 322,034 + GFF585 0.86 -0.9 322,092 + GFF585 0.90 +0.1 322,099 - -0.6 322,114 - +0.0 322,115 + -0.1 322,116 - +0.2 322,209 + -0.2 322,210 - +0.1 322,210 - -0.3 322,343 + GFF586 0.17 -0.2 322,349 + GFF586 0.18 +0.1 322,349 + GFF586 0.18 -1.9 322,438 + GFF586 0.35 +0.5 322,642 - GFF586 0.72 +0.2 322,670 + GFF586 0.77 -1.0 322,733 - GFF586 0.89 -0.4 322,751 + +0.0 322,752 - +0.1 322,763 + +0.3 322,763 + +0.1 322,800 + +0.5 322,801 - +0.1 322,801 - -0.1 322,822 - +0.3 322,842 - +0.2 322,950 + +0.0 323,100 - GFF587 0.17 +0.2 323,463 - GFF587 0.41 -1.1 323,524 - GFF587 0.45 -2.5 323,525 + GFF587 0.45 -0.5 323,784 + GFF587 0.63 -1.3 323,865 + GFF587 0.68 -1.2 324,027 - GFF587 0.79 -1.7 324,191 - GFF587 0.90 -0.7 324,451 + -0.0 324,452 - +0.7 324,455 + -0.1 324,455 + +0.5 324,456 - +0.1 324,456 - -0.9 324,457 + +0.4 324,476 + +0.1 324,489 + -0.6 324,490 - -0.3 324,512 - -0.4 324,564 + -0.3 324,564 + +0.7 324,564 + -0.2 324,565 - +0.4 324,584 - GFF588 0.11 +0.2 324,735 - GFF588 0.57 -0.4 324,743 - GFF588 0.60 -0.8 324,756 + GFF588 0.64 +0.2 324,776 - GFF588 0.70 -0.0 324,793 + GFF588 0.75 +0.1 324,909 - -0.4 324,910 + -0.1 324,910 + +0.1 324,910 + -0.2 324,910 + -0.4 324,910 + -0.1 324,910 + +0.0 324,910 + -0.3 324,910 + -0.3 324,910 + +0.4 324,911 - +1.0 324,911 - -0.6 324,911 - -0.9 324,911 - -2.4 324,911 - -0.2 324,911 - -0.3 324,911 - -0.4 324,911 - -0.2 324,911 - +0.4 324,912 + -0.3 324,912 + -0.2 324,913 - -0.7 324,913 - -0.4 324,913 - -0.1 324,913 - -0.9 324,927 + +0.5 325,004 - +0.4 325,029 + -0.0 325,056 + GFF589 0.13 -0.1 325,270 + GFF589 0.39 -0.1 325,270 + GFF589 0.39 +0.9 325,271 - GFF589 0.39 -1.2
Or see this region's nucleotide sequence