Experiment: Acanthamoeba sp., muxed culture-1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4763 and GFF4764 overlap by 4 nucleotides GFF4764 and GFF4765 are separated by 140 nucleotides
GFF4763 - Type III secretion outermembrane contact sensing protein (YopN,Yop4b,LcrE), at 70,265 to 71,383
GFF4763
GFF4764 - Type III secretion outermembrane pore forming protein (YscC,MxiD,HrcC, InvG), at 71,380 to 72,924
GFF4764
GFF4765 - Type III secretion thermoregulatory protein (LcrF,VirF,transcription regulation of virulence plasmid), at 73,065 to 73,814
GFF4765
Position (kb)
71
72
73 Strain fitness (log2 ratio)
-1
0
1 at 70.530 kb on - strand, within GFF4763 at 70.601 kb on + strand, within GFF4763 at 70.753 kb on - strand, within GFF4763 at 70.753 kb on - strand, within GFF4763 at 70.753 kb on - strand, within GFF4763 at 70.829 kb on + strand, within GFF4763 at 71.009 kb on + strand, within GFF4763 at 71.010 kb on - strand, within GFF4763 at 71.010 kb on - strand, within GFF4763 at 71.076 kb on - strand, within GFF4763 at 71.084 kb on + strand, within GFF4763 at 71.302 kb on + strand at 71.302 kb on + strand at 71.302 kb on + strand at 71.302 kb on + strand at 71.431 kb on + strand at 71.566 kb on - strand, within GFF4764 at 71.592 kb on + strand, within GFF4764 at 71.704 kb on - strand, within GFF4764 at 71.738 kb on - strand, within GFF4764 at 71.748 kb on + strand, within GFF4764 at 71.748 kb on + strand, within GFF4764 at 71.748 kb on + strand, within GFF4764 at 71.803 kb on + strand, within GFF4764 at 71.804 kb on - strand, within GFF4764 at 71.852 kb on + strand, within GFF4764 at 71.852 kb on + strand, within GFF4764 at 71.852 kb on + strand, within GFF4764 at 71.857 kb on + strand, within GFF4764 at 71.857 kb on + strand, within GFF4764 at 71.857 kb on + strand, within GFF4764 at 71.858 kb on - strand, within GFF4764 at 71.877 kb on + strand, within GFF4764 at 71.877 kb on + strand, within GFF4764 at 71.877 kb on + strand, within GFF4764 at 71.877 kb on + strand, within GFF4764 at 71.878 kb on - strand, within GFF4764 at 71.878 kb on - strand, within GFF4764 at 71.878 kb on - strand, within GFF4764 at 72.037 kb on + strand, within GFF4764 at 72.078 kb on + strand, within GFF4764 at 72.083 kb on - strand, within GFF4764 at 72.083 kb on - strand, within GFF4764 at 72.270 kb on + strand, within GFF4764 at 72.271 kb on - strand, within GFF4764 at 72.399 kb on + strand, within GFF4764 at 72.400 kb on - strand, within GFF4764 at 72.459 kb on + strand, within GFF4764 at 72.507 kb on + strand, within GFF4764 at 72.598 kb on - strand, within GFF4764 at 72.627 kb on - strand, within GFF4764 at 72.676 kb on + strand, within GFF4764 at 72.765 kb on - strand, within GFF4764 at 72.985 kb on + strand at 73.011 kb on + strand at 73.301 kb on - strand, within GFF4765 at 73.362 kb on + strand, within GFF4765 at 73.410 kb on + strand, within GFF4765 at 73.416 kb on - strand, within GFF4765 at 73.534 kb on + strand, within GFF4765 at 73.600 kb on - strand, within GFF4765 at 73.715 kb on + strand, within GFF4765 at 73.716 kb on - strand, within GFF4765
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-1 remove 70,530 - GFF4763 0.24 -0.2 70,601 + GFF4763 0.30 -0.3 70,753 - GFF4763 0.44 +0.5 70,753 - GFF4763 0.44 +1.0 70,753 - GFF4763 0.44 +1.3 70,829 + GFF4763 0.50 -0.3 71,009 + GFF4763 0.66 -0.3 71,010 - GFF4763 0.67 +0.8 71,010 - GFF4763 0.67 +1.5 71,076 - GFF4763 0.72 +0.4 71,084 + GFF4763 0.73 -0.1 71,302 + +0.6 71,302 + +0.1 71,302 + +1.6 71,302 + +0.5 71,431 + -0.6 71,566 - GFF4764 0.12 +0.3 71,592 + GFF4764 0.14 +0.3 71,704 - GFF4764 0.21 +1.7 71,738 - GFF4764 0.23 -0.8 71,748 + GFF4764 0.24 -0.8 71,748 + GFF4764 0.24 -0.3 71,748 + GFF4764 0.24 -0.7 71,803 + GFF4764 0.27 -0.3 71,804 - GFF4764 0.27 -0.8 71,852 + GFF4764 0.31 +0.2 71,852 + GFF4764 0.31 -0.1 71,852 + GFF4764 0.31 -0.3 71,857 + GFF4764 0.31 -0.6 71,857 + GFF4764 0.31 +0.3 71,857 + GFF4764 0.31 -0.2 71,858 - GFF4764 0.31 -0.1 71,877 + GFF4764 0.32 +0.0 71,877 + GFF4764 0.32 -1.1 71,877 + GFF4764 0.32 -0.2 71,877 + GFF4764 0.32 +0.5 71,878 - GFF4764 0.32 +0.2 71,878 - GFF4764 0.32 +0.7 71,878 - GFF4764 0.32 +0.8 72,037 + GFF4764 0.43 +0.4 72,078 + GFF4764 0.45 -0.6 72,083 - GFF4764 0.46 +0.0 72,083 - GFF4764 0.46 -0.8 72,270 + GFF4764 0.58 -0.2 72,271 - GFF4764 0.58 +0.7 72,399 + GFF4764 0.66 -0.5 72,400 - GFF4764 0.66 +0.0 72,459 + GFF4764 0.70 +0.3 72,507 + GFF4764 0.73 +0.2 72,598 - GFF4764 0.79 +0.1 72,627 - GFF4764 0.81 -0.4 72,676 + GFF4764 0.84 -0.3 72,765 - GFF4764 0.90 -0.0 72,985 + +0.2 73,011 + -1.6 73,301 - GFF4765 0.31 -0.1 73,362 + GFF4765 0.40 -0.0 73,410 + GFF4765 0.46 -0.3 73,416 - GFF4765 0.47 -0.6 73,534 + GFF4765 0.63 +0.5 73,600 - GFF4765 0.71 -0.3 73,715 + GFF4765 0.87 +0.2 73,716 - GFF4765 0.87 +0.9
Or see this region's nucleotide sequence