Experiment: Acanthamoeba sp., muxed culture-1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4587 and GFF4588 are separated by 210 nucleotides GFF4588 and GFF4589 are separated by 35 nucleotides GFF4589 and GFF4590 are separated by 296 nucleotides
GFF4587 - PTS system, N-acetylglucosamine-specific IIA component (EC 2.7.1.69) / PTS system, N-acetylglucosamine-specific IIB component (EC 2.7.1.69) / PTS system, N-acetylglucosamine-specific IIC component (EC 2.7.1.69), at 375,802 to 377,754
GFF4587
GFF4588 - Glutaminyl-tRNA synthetase (EC 6.1.1.18), at 377,965 to 379,632
GFF4588
GFF4589 - hypothetical protein, at 379,668 to 379,781
GFF4589
GFF4590 - N-acetylglucosamine-regulated outer membrane porin, at 380,078 to 381,484
GFF4590
Position (kb) 377
378
379
380 Strain fitness (log2 ratio) -1
0
1 at 376.987 kb on + strand, within GFF4587 at 376.988 kb on - strand, within GFF4587 at 376.988 kb on - strand, within GFF4587 at 376.988 kb on - strand, within GFF4587 at 377.037 kb on + strand, within GFF4587 at 377.038 kb on - strand, within GFF4587 at 377.040 kb on + strand, within GFF4587 at 377.041 kb on - strand, within GFF4587 at 377.439 kb on + strand, within GFF4587 at 377.511 kb on + strand, within GFF4587 at 377.645 kb on + strand at 377.646 kb on - strand at 377.722 kb on - strand at 377.735 kb on - strand at 377.917 kb on - strand at 377.918 kb on + strand at 377.918 kb on + strand at 377.926 kb on + strand at 377.936 kb on + strand at 379.609 kb on - strand at 379.930 kb on + strand at 380.072 kb on + strand at 380.072 kb on + strand at 380.073 kb on - strand at 380.101 kb on + strand at 380.104 kb on + strand at 380.213 kb on + strand at 380.272 kb on + strand, within GFF4590 at 380.501 kb on + strand, within GFF4590 at 380.588 kb on + strand, within GFF4590 at 380.588 kb on + strand, within GFF4590
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-1 remove 376,987 + GFF4587 0.61 -0.3 376,988 - GFF4587 0.61 -0.9 376,988 - GFF4587 0.61 +0.1 376,988 - GFF4587 0.61 +0.2 377,037 + GFF4587 0.63 -0.2 377,038 - GFF4587 0.63 -0.2 377,040 + GFF4587 0.63 +0.6 377,041 - GFF4587 0.63 +0.5 377,439 + GFF4587 0.84 +0.6 377,511 + GFF4587 0.88 -0.2 377,645 + +0.2 377,646 - +0.5 377,722 - -0.1 377,735 - -0.3 377,917 - -0.3 377,918 + +0.5 377,918 + -1.2 377,926 + +0.6 377,936 + -0.4 379,609 - +0.1 379,930 + -0.0 380,072 + -0.1 380,072 + +0.2 380,073 - +0.6 380,101 + +0.9 380,104 + -0.2 380,213 + -0.3 380,272 + GFF4590 0.14 -1.1 380,501 + GFF4590 0.30 +0.8 380,588 + GFF4590 0.36 -0.2 380,588 + GFF4590 0.36 -0.3
Or see this region's nucleotide sequence